Predicting the Efficacy of Resistance Exercise Training for Improving Endurance Performance

Elite Cyclists Clinical Trial

— IDperform  

Official title:

Identifying Molecular Determinants for the Efficacy of Resistance Training for Improving Endurance Performance in Elite Cyclists

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04637178
• Other study ID #: Trainome 2020#025-2
• Status: Completed
• Phase: N/A
• Start date: November 16, 2020
• Est. completion date: February 26, 2021

Study information

• Verified date: November 2020
• Source: Inland Norway University of Applied Sciences
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The overall objective of the study is to identify molecular determinants of the efficacy of resistance training for improving endruance performance in elite cyclists

Description:

For elite endurance athletes, the addition of resistance training to habitual endurance training routines generally leads to improved endurance performance. However, this is not always the case, with several individuals failing to respond in the expected manner. This inter-individual variation is likely associated with inherent cellular and molecular charateristics of skeletal muscle. Indeed, responses to resistance training seem to be associated with muscle biological characteristics such as muscle fiber composition and ribosomal content (during the early phase of resistance training).

The study will investigate the association between muscle fibre composition (type I vs type II muscle fiber proportions) / ribosomal content in m. vastus lateralis and the effects of 10 weeks of resistance training on endurance performance in 52 elite cyclists. Half the participants will perform resistance training in addition to their habitual endurance training routines, the other half will perform endurance training-only. Muscle biopsies will be sampled from m. vastus lateralis before and after the first two weeks of the intervention. For muscle fiber composition, the hypothesis is that larger proportions of type II fibers will be associated with more pronounced benefits of resistance training. For ribosomal content, the hypothesis is that higher rRNA content at baseline and larger increases in rRNA content during the initial part of the intervention will be associated with more pronounced benefits of resistance training. No changes are expected in the non-resistance training control group.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 25
• Est. completion date: February 26, 2021
• Est. primary completion date: February 26, 2021
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 45 Years

Eligibility

Inclusion criteria:

• VO2max > 65 ml/kg/min (male participants)

• VO2max > 50 ml/kg/min (female participants)

• >7 hours of endurance training per week for the 6 months leading up to the study

Exclusion criteria:

• having conducted regular resistance training for the last 6 months leading up to the study

Related Conditions & MeSH terms

• Elite Cyclists

Intervention

Behavioral:
• Resistance training
Resistance training sessions twice a week for ten weeks
• Endurance training (habitual)
Endurance training for ten weeks (habitual routines)

Locations

Country	Name	City	State
Norway	Inland Norway University of Applied Sciences	Lillehammer	Inland Norway

Sponsors (1)

Lead Sponsor	Collaborator
Inland Norway University of Applied Sciences

Country where clinical trial is conducted

Norway, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Endurance training volume	Self-reported endurance training volume measured as time-spent in different exercise intensity zones	Throughout study completion (daily), an average of ten weeks
Other	Resistance training volume	Self-reported resistance training volume measured as load x repetitions	Throughout study completion (daily), an average of ten weeks
Primary	Performance during a 40-minute all-out cycling test	Mean power output measured during a 40-minute all-out cycling test	Changes from before the intervention to immediately after the intervention
Primary	Performance during a 15-minute all-out cycling test	Mean power output measured during a 15-minute all-out cycling test	Changes from before the intervention to immediately after the intervention
Secondary	Total RNA in muscle tissue (before intervention)	Total RNA content in m. vastus lateralis (ug per mg tissue)	Before the intervention
Secondary	Total RNA in muscle tissue (changes after two weeks)	Total RNA content in m. vastus lateralis (ug per mg tissue)	Changes from before the intervention to immediately after the initial two weeks of resistance training
Secondary	Muscle fiber proportions (before intervention)	Muscle fiber proportions in m. vastus lateralis measured using immunohistochemistry	Before the intervention
Secondary	Muscle fiber proportions (changes after two weeks)	Muscle fiber proportions in m. vastus lateralis measured using immunohistochemistry	Changes from before the intervention to immediately after the initial two weeks of resistance training
Secondary	Muscle fiber cross-sectional area (before intervention)	Cross-sectional area of muscle fibres in m. vastus lateralis measured using immunohistochemistry	Before the intervention
Secondary	Muscle fiber cross-sectional area (changes after two weeks)	Cross-sectional area of muscle fibres in m. vastus lateralis measured using immunohistochemistry	Changes from before the intervention to immediately after the initial two weeks of resistance training
Secondary	Myonuclear number (before intervention)	Myonuclei per muscle fiber in m. vastus lateralis measured using immunohistochemistry	Before the intervention
Secondary	Myonuclear number (changes after two weeks)	Myonuclei per muscle fiber in m. vastus lateralis measured using immunohistochemistry	Changes from before the intervention to immediately after the initial two weeks of resistance training
Secondary	rRNA expression (before intervention)	rRNA abundances in m. vastus lateralis measured using qPCR	Before the intervention
Secondary	rRNA expression (changes after two weeks)	rRNA abundances in m. vastus lateralis measured using qPCR	Changes from before the intervention to immediately after the initial two weeks of resistance training
Secondary	mRNA expression (before intervention)	mRNA abundances in m. vastus lateralis measured using qPCR	Before the intervention
Secondary	mRNA expression (changes after two weeks)	mRNA abundances in m. vastus lateralis measured using qPCR	Changes from before the intervention to immediately after the initial two weeks of resistance training
Secondary	Maximal concentric force production	Maximal concentric force production measured using a seated leg press test	Changes from before the intervention to immediately after the intervention
Secondary	Sprint performance	Mean power output measured during a 10-second all-out cycling sprint	Changes from before the intervention to immediately after the intervention
Secondary	Maximal oxygen consumption	Maximal oxygen consumption measured during an incremental cycling exercise test to exhaustion	Changes from before the intervention to immediately after the intervention
Secondary	Maximal aerobic power output	Maximal aerobic power output measured as mean power output during the last minute of an incremental cycling exercise test to exhaustion	Changes from before the intervention to immediately after the intervention
Secondary	Power output at lactate threshold	Power output at 4 mmol blood lactate concentration measured during an incremental cycling exercise test (with 5 minute steps)	Changes from before the intervention to immediately after the intervention
Secondary	Gross efficiency	Contribution of total energy turnover to power output in the fresh and fatigued state	Changes from before the intervention to immediately after the intervention
Secondary	Fractional utilization of VO2max (incremental test)	Fractional utilization of VO2max measured at 4 mmol blood lactate concentrations measured during an incremental cycling exercise test (with 5 minute steps)	Changes from before the intervention to immediately after the intervention
Secondary	Fractional utilization of VO2max (15-minute performance test)	Fractional utilization of VO2max measured during a 15-minute performance test	Changes from before the intervention to immediately after the intervention
Secondary	Hemoglobin mass	Hemoglobin mass measured using CO rebreathing (g)	Changes from before the intervention to immediately after the intervention
Secondary	Blood volume	Blood volume measured using CO rebreathing	Changes from before the intervention to immediately after the intervention
Secondary	Plasma volume	Plasma volume measured using CO rebreathing	Changes from before the intervention to immediately after the intervention
Secondary	Red blood cell volume	Red blood cell volume measured using CO rebreathing	Changes from before the intervention to immediately after the intervention
Secondary	Hematocrit	Hematocrit measured using centrifugation	Changes from before the intervention to immediately after the intervention