SEGATROM: Sensory and Gastrointestinal Impact of Taste Receptor Variants on Human Metabolism and Nutrition

Disorder of Glucose Regulation Clinical Trial

— SEGATROM  

Official title:

Sensory and Gastrointestinal Impact of Taste Receptor Variants on Human Metabolism and Nutrition

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02219295
• Other study ID #: BMBF 11005D
• Status: Completed
• Phase: N/A
• Start date: December 2011
• Est. completion date: September 2014

Study information

• Verified date: June 2020
• Source: German Institute of Human Nutrition
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

We investigate the impact of taste receptors (sweet, bitter and umami) on human metabolism. There ist growing evidence, that components of the taste signalling system, including the taste receptor proteins , have important functions in the regulation of metabolic parameters in non-gustatory tissues.

Description:

We apply different sweet, bitter and umami taste compounds in a single dose in the human intervention. We use substances as for instance glucose, sucrose,saccharin and lactisol in the sweet part, and substances from cabbage, beer or red endive in the bitter part. For umami we take glutamate.

Blood samples are collected at baseline and 6 times thereafter up to 3hours, to analyse the incretins GLP-1(glucagon-like peptide 1) and GIP(gastrointestinal polypeptide) and other peptide hormones as insulin and PYY(peptide tyrosin trosin) in human beings.

Gastrointestinal mucosa is taken by gastroscopy from the lower duodenum from study subjects for analysis for the presence or absence of functional taste receptors in gastrointestinal tissues.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 97
• Est. completion date: September 2014
• Est. primary completion date: September 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 75 Years

Eligibility

Inclusion Criteria:

• Healthy persons

Exclusion Criteria:

• Tumor in history

• Pregnancy

• Severe illness in history

• Bodymass index below 18

Related Conditions & MeSH terms

• Disorder of Glucose Regulation

Intervention

Dietary Supplement:
• sweet block
oral application of the above mentioned substances in 300 ml tap water in 5 min. , collection of blood at 0,15,30, 60 ,120,180 minutes for measurement of incretins GIP(gastrointestinal polypeptide) and GLP-1 (glucagon like peptide 1), Protein hormones PYY(Peptide YY),CCK (cholecystokinin), Leptin and Insulin
• bitter block
oral application of the above mentioned substances in 300 ml tap water, collection of blood samples at -15,0,15,30,60,120,180 min for Insulin, GIP,GLP-1, PYY and glucose
• umami-block
oral application of umami substances as glutamate with and without IMP, collection of blood samples as above

Other:
• gastroscopy
gastroscopy with and without oral application of saccharin, tissue samples from th upper duodenum by endoscopy for the detection of taste receptor cells in the upper bowel

Locations

Country	Name	City	State
Germany	Department of Clinical Nutrition	Bergholz-Rehbrücke	Brandenburg

Sponsors (2)

Lead Sponsor	Collaborator
German Institute of Human Nutrition	Technische Universität München

Country where clinical trial is conducted

Germany, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Changes in the Area under the Curve (AUC) for PYY in different bitter tasting substances	oral application of sinigrin, lactucopicrin and other extracts of vegetables and umami, getting insight into the different bitter receptors	3 years
Primary	Area under the curve (AUC): changes of expression levels of the sweet , bitter und umami taste receptors in humans in the upper bowel and responses of incretins to receptor agonists and antagonists	measurement of incretins (GIP, GLP-1) and peptide hormones in human plasma after consumption of different sugars , sweeteners , bitter tasting ingredients of vegetables und umami. Measurement of expression levels in human intestinal mucosa before and after receptor stimulation	three years
Secondary	changes in the signalling cascade of the sweet receptor SGLT1 (sodium tranporter 1), changes of receptor expression	oral application of sweet tasting substances and antagonists, endoscopic collection of tissue samples, determination of molecular resonses by qRT-PCR and immunohistochemistry	3 years