Regeneration of Bronchial Epithelium During Chronic Obstructive Pulmonary Disease

COPD Clinical Trial

— EPITHREGENCOPD  

Official title:

Regeneration of Bronchial Epithelium During Chronic Obstructive Pulmonary Disease

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02873988
• Other study ID #: PA11017
• Status: Completed
• Phase: N/A
• First received: August 10, 2016
• Last updated: August 16, 2016
• Start date: March 2012
• Est. completion date: May 2014

Study information

• Verified date: August 2016
• Source: CHU de Reims
• Contact: n/a
• Is FDA regulated: No
• Health authority: France: Commission nationale de l'informatique et des libertés
• Study type: Observational

Clinical Trial Summary

Airway epithelium integrity is essential to maintain its role of mechanical and functional barrier. Recurrent epithelial injuries require a complex mechanism of repair to restore its integrity. In chronic obstructive pulmonary disease (COPD), an abnormal airway epithelial repair may participate in airway remodelling. The objective was to determine if airway epithelial wound repair of airway epithelium is abnormal in COPD.

Description:

Patients scheduled for lung resection were prospectively recruited. Demographic, clinical data and pulmonary function tests results were recorded. Emphysema was visually scored and histological remodelling features were noted. Primary bronchial epithelial cells (BEC) were extracted and cultured for wound closure assay. The investigators determined the mean speed of wound closure (MSWC) and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of BEC 18 hours after cell wounding. In a subset of patients, bronchiolar epithelial cells were also cultured for wound closure assay for MSWC analyze.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 25
• Est. completion date: May 2014
• Est. primary completion date: December 2013
• Accepts healthy volunteers: No
• Gender: Both
• Age group: 40 Years and older

Eligibility

Inclusion Criteria:

6 Patients scheduled for lung resection for cancer

Exclusion Criteria:

• Patients with asthma, cystic fibrosis, bronchiectasis or pulmonary fibrosis were excluded

Study Design

Observational Model: Case Control, Time Perspective: Cross-Sectional

Related Conditions & MeSH terms

• COPD
• Lung Diseases
• Lung Diseases, Obstructive
• Pulmonary Disease, Chronic Obstructive

Intervention

Other:
• bronchial epithelial cell culture

Locations

Country	Name	City	State
France	Chu de Reims	Reims

Sponsors (1)

Lead Sponsor	Collaborator
CHU de Reims

Country where clinical trial is conducted

France, 

References & Publications (1)

Perotin JM, Adam D, Vella-Boucaud J, Delepine G, Sandu S, Jonvel AC, Prevost A, Berthiot G, Pison C, Lebargy F, Birembaut P, Coraux C, Deslee G. Delay of airway epithelial wound repair in COPD is associated with airflow obstruction severity. Respir Res. 2014 Nov 27;15:151. doi: 10.1186/s12931-014-0151-9. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Mean speed of wound closure (MSWC) of wounded bronchial epithelial cell culture obtained from COPD and control patients		hour 18	No
Secondary	Cell proliferation index in supernatants of bronchial epithelial cells 18 hours after cell wounding.		hour 18	No
Secondary	Matrix metalloprotease (MMP)-2 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.		hour 18	No
Secondary	Matrix metalloprotease (MMP)-9 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.		hour 18	No
Secondary	Cytokines levels in supernatants of bronchial epithelial cells 18 hours after cell wounding.		hour 18	No