Impact of COPD on Muscle Regeneration

COPD Clinical Trial

Official title:

Impact of Chronic Obstructive Pulmonary Disease on Peripheral and Respiratory Muscle Regeneration

Clinical Trial Details — Status: Active, not recruiting

Administrative data

• NCT number: NCT02557165
• Other study ID #: CRT114804
• Status: Active, not recruiting
• Phase: N/A
• First received: September 2, 2015
• Last updated: September 21, 2015
• Start date: January 2011
• Est. completion date: December 2015

Study information

• Verified date: September 2015
• Source: Laval University
• Contact: n/a
• Is FDA regulated: No
• Health authority: Canada: Ministère santé et services sociaux du Québec
• Study type: Observational

Clinical Trial Summary

Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD) and can affect both respiratory and peripheral muscles. Impaired muscle regeneration is thought to be implicated in the process of muscle atrophy. In this study the investigators will evaluate muscle regenerative potential of the Vastus Lateralis (VL) and diaphragm of patients with COPD, compared to patients with normal lung function.

Description:

Preliminary results suggest that the activation and differentiation processes of satellite cells isolated from the VL of patients with COPD could be altered. The investigators propose to compare the regenerative potential of the VL and the diaphragm in 15 patients with COPD and 15 individuals with normal lung function undergoing a thoracic surgery. From muscle biopsies, satellite cells will be isolated and cultured to assess their proliferation and differentiation rates, and the expression level of myogenic effectors (MyoD, Myf5, myogenin, and MRF4). Alterations in these measurements will confirm the hypotheses that the regenerative potential is lower in both muscle groups in patients with COPD compared to controls and that in COPD, the regenerative potential is lower in VL compared to the diaphragm.

Recruitment information / eligibility

• Status: Active, not recruiting
• Enrollment: 27
• Est. completion date: December 2015
• Est. primary completion date: June 2015
• Accepts healthy volunteers: No
• Gender: Both
• Age group: 40 Years to 80 Years

Eligibility

Inclusion Criteria:

• Patient with a scheduled thoracic surgery

• = 40 years

• Smoking cessation = 1 month

• Smoking history = 10 pack-year

Exclusion Criteria:

• Subjects who received either chemiotherapy or radiotherapy

• Hypoxemia

• Body weight loss = 10%

• Use of systemic corticosteroid in the last month

Study Design

Observational Model: Case-Crossover, Time Perspective: Prospective

Related Conditions & MeSH terms

• COPD

Intervention

Procedure:
• Muscle biopsies
Muscle biopsies will allow acces to samples for observational studies. Vastus latetalis: needle biopsy (Bergstrom technique). Diaphragm: open biopsy performed by thoracic surgeon during lung surgery.

Locations

Country	Name	City	State
n/a

Sponsors (2)

Lead Sponsor	Collaborator
Laval University	GlaxoSmithKline

References & Publications (3)

Caron MA, Debigaré R, Dekhuijzen PN, Maltais F. Comparative assessment of the quadriceps and the diaphragm in patients with COPD. J Appl Physiol (1985). 2009 Sep;107(3):952-61. doi: 10.1152/japplphysiol.00194.2009. Epub 2009 Apr 9. Review. — View Citation

Thériault ME, Paré MÈ, Lemire BB, Maltais F, Debigaré R. Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease. Respir Res. 2014 Mar 25;15:35. doi: 10.1186/1465-9921-15-35. — View Citation

Thériault ME, Paré MÈ, Maltais F, Debigaré R. Satellite cells senescence in limb muscle of severe patients with COPD. PLoS One. 2012;7(6):e39124. doi: 10.1371/journal.pone.0039124. Epub 2012 Jun 13. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Muscle satellite cell proliferation index	Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the proliferation capacity	Sequential analyses performed over a period of 2 weeks after tissue collection.	No
Primary	Muscle satellite cell differentiation index	Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the differentiation capacity	Sequential analyses performed over a period of 2 months after tissue collection.	No