Identification of EMT Indicators in Healthy and Periodontally Diseased Gingival Samples

Chronic Periodontitis Clinical Trial

Official title:

Localization of Indicators for Epithelial Mesenchymal Transformation in Gingiva From Chronic Periodontitis Patients as Compared to Healthy Individuals (Case Control Study)

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT03462953
• Other study ID #: CEBC-CU-2018-02-28
• Status: Not yet recruiting
• Phase: N/A
• First received: March 4, 2018
• Last updated: March 9, 2018
• Start date: April 15, 2018
• Est. completion date: March 15, 2019

Study information

• Verified date: March 2018
• Source: Cairo University
• Contact: Karim ghobrial, bachelor
• Phone: 01203346668
• Email: karimwasfi3[@]gmail.com
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The current study focuses on the localization and quantitative assessment of growth factors and cytokines related to the EMT process found in the human gingival tissue samples taken from periodontally diseased individuals compared to other samples taken from healthy individuals. Through this investigation the correlation between the severity of the disease and the amount of these factors will be studied aiming to alleviation of the high prevalence of periodontal diseases among the Egyptian population.

Description:

In a case control human study The existence and the amount of the indicators and cytokines for EMT in the gingival tissue samples taken from the periodontitis patients will be investigated and compared to those in healthy individuals using histopathology and real time PCR to reveal the role of these factors in periodontal disease incidence in humans.

Total of 36 participants will be recruited in this study The participants will be divided according to their periodontal status into four groups (healthy, mild, moderate and severe periodontitis) 9 patients in each group.

The periodontal screening of patients will be done using mouth mirror and graduated Williams's periodontal probe for the following:

• Gingival status will be assessed by using gingival index

• Periodontal pocket depth.

• Clinical attachment loss.

• Radiographic examination using panoramic technique. Screening criteria for chronic periodontitis was the presence of at least 5 sites with 4 mm horizontal alveolar bone loss on radiographs.

Gingival tissue samples will be harvested during periodontal surgery and extraction of the periodontally hopeless tooth for the periodontitis patients and during the premolar extraction (Orthodontic ttt) or third molar extraction for the healthy controls.

The dissected gingival samples will be washed in saline solution and fixed in 4% buffered formalin for 48 hours, dehydrated in ascending grades of ethyl alcohol, cleared in xylene and embedded in paraffin and will be subjected to the following:.

1. Sections of 4-5 µm in thickness will be obtained, deparaffinized, rehydrated and subjected to histological staining and histomorphometric analysis.

2. Real time- PCR for TGF-B1 ,Vimenten , E-Cadherin .

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 36
• Est. completion date: March 15, 2019
• Est. primary completion date: August 15, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 21 Years and older

Eligibility

Inclusion Criteria:

• Inclusion criteria for healthy group:

• Absence of any clinical sign of gingival inflammation

• Probing depth =3 mm

• No clinical attachment loss.

Inclusion criteria for periodontitis group:

• Generalized presence of clinical signs of gingival inflammation

• Generalized probing depth =4 mm

• Generalized clinical attachment loss of =2 mm.

Exclusion Criteria:

• • Patients with any systemic disease,

• Smokers

• Pregnant or lactating women,

• Cervical/proximal/subgingival caries or restorations,

• Periodontal or antimicrobial therapy within 3 months before sampling.

Related Conditions & MeSH terms

• Chronic Periodontitis
• Periodontitis

Intervention

Procedure:
• Gingival tissue samples will be harvested
Gingival tissue samples will be harvested during the premolar extraction (Orthodontic ttt) or third molar extraction for the healthy controls and during periodontal surgery or extraction of hopeless teeth with periodontal disease .. The dissected gingival samples will be washed in saline solution and fixed in 4% buffered formalin for 48 hours, dehydrated in ascending grades of ethyl alcohol, cleared in xylene and embedded in paraffin and will be subjected to the following:. Sections of 4-5 µm in thickness will be obtained, deparaffinized, rehydrated and subjected to histological staining and histomorphometric analysis. Real time- PCR for TGF-B1 ,Vimenten , E-Cadherin .

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Cairo University

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Localization of EMT indicators TGF- ß1, Vimenten, E-Cadherin	using real time PCR the EMT indicators will be identified and quantified in all the gingival samples taken from patients of both arms of the study	one year