Chronic Periodontitis Clinical Trial
Official title:
Localization of Indicators for Epithelial Mesenchymal Transformation in Gingiva From Chronic Periodontitis Patients as Compared to Healthy Individuals (Case Control Study)
The current study focuses on the localization and quantitative assessment of growth factors and cytokines related to the EMT process found in the human gingival tissue samples taken from periodontally diseased individuals compared to other samples taken from healthy individuals. Through this investigation the correlation between the severity of the disease and the amount of these factors will be studied aiming to alleviation of the high prevalence of periodontal diseases among the Egyptian population.
In a case control human study The existence and the amount of the indicators and cytokines
for EMT in the gingival tissue samples taken from the periodontitis patients will be
investigated and compared to those in healthy individuals using histopathology and real time
PCR to reveal the role of these factors in periodontal disease incidence in humans.
Total of 36 participants will be recruited in this study The participants will be divided
according to their periodontal status into four groups (healthy, mild, moderate and severe
periodontitis) 9 patients in each group.
The periodontal screening of patients will be done using mouth mirror and graduated
Williams's periodontal probe for the following:
- Gingival status will be assessed by using gingival index
- Periodontal pocket depth.
- Clinical attachment loss.
- Radiographic examination using panoramic technique. Screening criteria for chronic
periodontitis was the presence of at least 5 sites with 4 mm horizontal alveolar bone
loss on radiographs.
Gingival tissue samples will be harvested during periodontal surgery and extraction of the
periodontally hopeless tooth for the periodontitis patients and during the premolar
extraction (Orthodontic ttt) or third molar extraction for the healthy controls.
The dissected gingival samples will be washed in saline solution and fixed in 4% buffered
formalin for 48 hours, dehydrated in ascending grades of ethyl alcohol, cleared in xylene and
embedded in paraffin and will be subjected to the following:.
1. Sections of 4-5 µm in thickness will be obtained, deparaffinized, rehydrated and
subjected to histological staining and histomorphometric analysis.
2. Real time- PCR for TGF-B1 ,Vimenten , E-Cadherin .
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