Clinical Trial Details
— Status: Completed
Administrative data
| NCT number |
NCT04763187 |
| Other study ID # |
LSMU123 |
| Secondary ID |
|
| Status |
Completed |
| Phase |
N/A
|
| First received |
|
| Last updated |
|
| Start date |
September 1, 2019 |
| Est. completion date |
January 29, 2020 |
Study information
| Verified date |
February 2021 |
| Source |
Lithuanian University of Health Sciences |
| Contact |
n/a |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Interventional
|
Clinical Trial Summary
Healing of post-extraction alveolus is a complex process that involves soft and hard tissues
regeneration. Pain, swelling, difficulty of opening the mouth, delayed healing of bone
tissue, alveolitis and horizontal or vertical resorption of bone tissue are the main problems
that have impact on consequent treatment. Blood concentrates PRGF (plasma rich in growth
factors) and PRF (platelet-rich fibrin), which are rich in growth factors, create better
conditions for post-extraction alveolus healing and enhance quality of soft tissues and bone
regeneration. Our study objective was to compare physiological healing of post-extraction
zone, PRF and PRGF induced changes of healing process. Methods that we used: 43 patients were
randomly divided into 3 groups: I control group - lower molar extraction and filling of
post-extraction alveolus with hemostatic sponge containing gentamicin, II group -
post-extraction alveolus is filled PRGF and III group - post-extraction alveolus is filled
with PRF. Bone regeneration was evaluated in CBCT scans after 1 month. Pain was evaluated
using visual analogical scale (VAS).
Description:
This was a prospective, single-centre, with unequal randomisation ratio [1,9:1:1], parallel,
multiple-group (control and two experimental groups) study. Randomized clinical trial was
conducted between September 2019 and December 2019 at department of Maxillofacial Surgery
Clinic of Kaunas Clinics. Permission of Bioethics Center was acquired for this study.
Participants of this study were patients who needed extraction of lower molars due to K04.5
(chronic apical periodontitis) and K01.0 (embedded teeth) diagnoses, based on ICD-10-CM
classification. In this study participated 43 patients: 33 (76,7%) women and 10 (23,3%) men,
average age - 28,6 years, the youngest was 18 years old and the oldest was 48 years old. All
patients were introduced to the study protocol and gave informed consent. Confidentiality of
subjects is assured because only summarized results will be published.
Patients were randomised into 3 groups: I control group - lower molar extraction and filling
of post-extraction alveolus with hemostatic sponge containing gentamicin, II group -
post-extraction alveolus is filled PRGF and III group - post-extraction alveolus is filled
with PRF. Randomization sequence was created using Excel 2019 (Microsoft, Redmond, WA, USA)
with a 1,9:1:1 allocation using random block sizes of 2 and 4. Surgery was performed by the
same surgeon. After procedures of tooth extraction and PRGF or PRF application, zone of
surgery was closed up using resorbable thread (Ethicon Coated Vicryl Plus 4-0) and applying
the method of cross mattress suture. During procedure control group was administred with
local antibiotics (gentamicin) to avoid infection possibility. Non-steroidal
anti-inflammatory medication was administered one hour after procedure and before sleep.
Threads were removed after 7 days. Pain felt on 1st and on 7th day after procedure was
evaluated using visual analogical scale (hereinafter - VAS) during the same appointment.
Pacients selected number from 0 to 10, there accordingly 0 - no pain, 5 - moderate pain and
10 - worst pain. [22] Cone beam computed tomography (hereinafter - CBCT) (E-WOO, Picasso
Trio, Republic of Korea) was carried out immediately after procedure and 1 month after
procedure for evaluation of bone tissue regeneration. It was decided to evaluate bone
regeneration in CBCT scans (Ez3D Plus Professional Ver.1.2.6.1) at following dimensions:
horizontal AA (X) (mm) and vertical AA (Y) (mm) dimensions of alveolar ridge were measured.
Also, horizontal X (mm), vertical Y (mm) and diagonal Z (mm) dimensions of primary bone
tissue formed in post-extraction alveolus in 1 month (Figure 2) All measurements carried out
by the same examinator.
Sample size of this study was calculated by using Paniotto formula. During the research
period (September to December 2019) was estimated that on average within 4 months 48 pacients
applies to department of Maxillofacial Surgery Clinic of Kaunas Clinics due to lower molars
extraction. After calculations with 95% probability and 0,05 error defined required sample
size - 43 pacients.
Statistical analysis carried out using SPSS (Statistical Package for the Social Science for
Windows; Chihago, USA) package 22.0. The diagnostic and demographic charasteristics were
compared using Wilcoxon test, Kruskal-Wallis test, Mann-Whitney test and Spearman correlation
analysis. Level of significance p was set at 0,05 for verification of statistical hypotheses.
Blood of 11 patients (3 men and 8 women, average age 27 years) taken from peripheral vein,
using 21G blood collection needle and PRGF-Endoret® test tubes with volume of 9 ml, for
production of PRGF. As per instruction of manufacturer, tubes contain 3,8 % sodium citrate,
which serves as anticoagulant. In order to produce the PRGF, test tubes were centrifuged for
8 minutes in 580 g Endoret® (PRGF®) System V centrifuge. 3 different layers are formed -
erythrocytes, leukocytes and blood plasma. Layer of blood plasma is divided into two
fractions. 1st fraction is called plasma poor in growth factors, which forms a top layer in a
tube down to 2nd fraction. 2nd fraction (2 ml of blood plasma, which is above the layer of
leukocytes), forms the layer of plasma rich in growth factors. Activation of this fraction is
carried out using sterile 10 % calcium chloride solution (50 µL of activator for 1 ml of
blood plasma), which carried out degranulation of platelets and therefore released growth
factors. After activation, test tubes were incubated at 37°C for 30 minutes, in order give
the consistency of gelatin to PRGF. Formed fold or fluid was inserted into alveolus of
extracted tooth, then the edges were closed up using resorbable cross mattress suture.
Blood of 11 patients (2 men and 9 women, average age 26 years) were collected from peripheral
vein using 21G blood collection needle and PRF test tubes with volume of 9 ml, for production
of PRF. Test tube was inserted into A-PRF centrifuge "A-PRF 12". The lid was closed and
program at 2800 rpm for 12 minutes was initiated. A-PRF fold was separated from red blood
cells (blood clots) in the test tube. After pulling the fold out of test tube, it was formed
on sterile surgical tray. Formed fold was inserted into alveolus of extracted tooth and edges
of wound were closed up using resorbable cross mattress suture.
