A Comparison Between Home-made Vitrification & Thawing Media and a Commercially Available Brand on Blastocyst

Blastocyst Disintegration Clinical Trial

Official title: A Comparison Between Home-made Vitrification & Thawing Media and a Commercially Available Brand on Blastocyst Survival, Expansion and Implantation

Status Completed

Clinical Trial Summary

This study was conducted to evaluate and compare cryo-survival, re-expansion, and implantation rates of Day 5/6 human blastocysts that were vitrified with home-made media (HM) according to (Shady grove protocol) and warmed with gradual Thawing HM according to (Kuwayama protocol)with Global Fast Freeze/Thaw Kits (LifeGlobal, Canada).

Clinical Trial Description

Group 1 blastocysts were vitrified with the Vit HM according to shady grove protocol using open system on cryotopvit. device and thawed with gradual thawing technique using HM according to Kuwayama protocol. Group 2 blastocysts were cryopreserved with the Global Fast Freeze Kitusing open system on cryotopvit. device and thawed using Gobal fast thaw kit. Blastocysts were subsequently cultured for 6-8 h, assessed for survival and expansion, then transferred and the percentages of Positive hCG, clinical pregnancy, implantation, multiple pregnancy and abortion were recorded. ;

Related Conditions & MeSH terms

• Blastocyst Disintegration
• Embryo Loss

• NCT number: NCT03660904
• Study type: Interventional
• Source: Benha University
• Status: Completed
• Phase: Phase 2/Phase 3
• Start date: October 1, 2015
• Completion date: June 15, 2018