Bacteria Infection Mechanism Clinical Trial
Official title:
The Thyroidectomy Wound Inflammation Can be Caused by Microbes Present in the Thyroid Parenchyma - Observational Research
The presence of cryptic microbes has been widely documented in animal healthy deep tissues.
The thyroid gland is an organ specifically exposed to the microbial environment due to its
close location to the mouth microbiome. A number of bacterial phenotypes has been detected in
the inflamed thyroid gland. A question raises as to whether bacteria have not already been
present in the thyroid gland before the clinical symptoms of goiter became evident.
A problem in thyroid surgery, relatively uncommon but difficult for control, is prolonged
thyroidectomy wound healing with skin flap, gland bed inflammation and fibrosis. The
causative bacteria may belong to the strains persistently present in the thyroid gland
parenchyma. Our objective is to answer questions: a) do the goiter tissue structures contain
bacteria, b) if so, which bacterial phenotypes can be identified, c) what are the genetic
similarities of the thyroid and periodontal bacterial strains.
Studies are carried out in patients with non-toxic multinodular goiter, toxic multinodular
goiter, Graves' disease, single adenoma, Hashimoto's disease, thyroid cancer and recurrent
thyroid disease. Tissue harvested during surgery is dissected immediately after thyroidectomy
into fragments of parenchyma, arteries, veins and lymph nodes and cultured on Columbia blood
agar base for up to 3 weeks. In this method bacteria present in the tissue grow in their
natural environment, slowly proliferate and then form the on-plate colonies. It enables
detection of even single bacteria usually difficult to be identified in planktonic media.
Identification of the isolated bacteria is performed. Their DNA patterns are also compared.
n/a
| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Completed |
NCT05234515 -
Bacteriology of sUrGical Site INfection Following Surgery for Intestinal Failure
|