Genetic Evolution of Arboviruses in New Caledonia Between 1995 and 2024 and Impact of Wolbachia — DENWOLUTION  

Dengue Clinical Trial

Official title: Genetic Evolution of Arboviruses in New Caledonia Between 1995 and 2024 and Impact of Wolbachia

Status Recruiting

Clinical Trial Summary

Arboviruses, diseases transmitted to humans by the bite of an insect vector, are a major public health problem, especially in tropical and sub-tropical countries. A promising strategy aimed at blocking the circulation of arboviruses is to release Aedes aegypti mosquitoes carrying the endosymbiotic bacterium Wolbachia. In 2019, the Wolbachia strategy was implemented in Nouméa as part of the World Mosquito Program. This intervention will modify the epidemiological profile of arboviruses in New Caledonia.

Epidemiological surveillance of arboviruses requires molecular characterization of the virus contained in the serum obtained from the blood collected from patients. This molecular characterization by RNA isolation techniques, RT-qPCR monitoring and sequencing allows the construction of phylogenetic trees.

In the context of the implementation of the World Mosquito Program in Nouméa, the investigators plan to follow the molecular evolution of arboviruses, over the period preceding the releases of mosquitoes carrying Wolbachia (from 1995 to 2019) then over a period of 5 years. following the releases.

At the same time, the virus can be isolated by cell culture techniques and in vitro infections, allowing its study in vitro in cells or in vivo in mosquitoes. This study allows us to measure the impact of the Wolbachia strategy on the evolution of the virus's ability to replicate in cells in the presence of Wolbachia and to be transmitted by the mosquito.

Clinical Trial Description

Collection of blood samples from patients with arbovirus infection

This study is a non-interventional study with retrospective part.

This study will improve :

• the assessment of the molecular evolution of arboviruses in the period preceding the implementation of the Wolbachia strategy

• the knowledge of the impact of Wolbachia on the diversity and molecular evolution of arboviruses

• the understanding of arbovirus transmission and replication mechanisms in the presence of Wolbachia ;

Related Conditions & MeSH terms

• Arbovirus Infections
• Chikungunya
• Dengue
• Zika

• NCT number: NCT04615364
• Study type: Observational
• Source: Institut Pasteur
• Contact: Myrielle Dupont-Rouzeyrol, PhD
• Phone: +687 27 75 30
• Email: mdupont@pasteur.nc
• Status: Recruiting
• Start date: July 17, 2020
• Completion date: July 17, 2025