In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines

Apoptosis Clinical Trial

— invitro  

Official title:

Inhibition and Cytotoxic Effects of Mangosteen on Cell Lines

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03728192
• Other study ID #: MAHER-MU-004-IEC/2016
• Status: Completed
• Phase: N/A
• Start date: March 7, 2016
• Est. completion date: July 13, 2018

Study information

• Verified date: October 2018
• Source: Meenakshi Ammal Dental College and Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The present study is an effort to investigate the hypothesis that in-vitro vitality and antiapoptotic effect of alcoholic crude extract of mangosteen on Oral cancer( H357) cell lines and Cevical cancer (HeLa) cell lines.

Description:

The oral and cervical cells were investigated by MTT (3-4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, DNA fragmentation detection by TUNEL (Terminal deoxynucleotidyl transferase-mediated d-UTP Nick End Labeling) assay and Apototic assay using Annexin V/FITC Kit.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 2
• Est. completion date: July 13, 2018
• Est. primary completion date: May 11, 2016
• Accepts healthy volunteers: No
• Gender: All
• Age group: N/A and older

Eligibility

Inclusion Criteria:

• Patients with oral squamous cell carcinoma

• Patients with cervical carcinoma

Exclusion Criteria:

• Patients with leukaemia

Related Conditions & MeSH terms

• Apoptosis

Intervention

Other:
• mangosteen extract
For Mangosteen group :DNA fragmentation assay is used to identify apoptosis.1×106 cells were harvested and treated with mangosteen pericarp extract for 48 h For Camptothecin group : Cells were also treated with standard anticancer drug Camptothecin to act as a positive control.

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Meenakshi Ammal Dental College and Hospital

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	apoptotic potential of ethanolic extract of mangosteen pericarp on oral and cervical cancer cell lines via apoptotic assay	Apoptosis assay was performed using Annexin V/FITC Kit (BD Biosciences, Catalog no. 556547), and the fluorescence intensities of FITC-conjugated annexin-V and Propidium iodide (PI) in cells were analyzed using flow cytometry. HeLa and H357 cells (1×106 cells/well) were seeded in a 6-well plate. The cells were allowed to adhere for 12 hrs, cultured in medium containing different concentrations of mangosteen extract for 48hr. The cells were then collected and washed twice with Phosphate buffer Saline, gently resuspended in 100µL annexinV-FITC binding buffer (1x) and incubated with 5µL annexinV-FITC in the dark for 10 min at 25°C. This was followed by centrifugation of cells at 2000 rpm for 5 min, and gently resuspended in 500µL annexinV-FITC binding buffer (1x) and 5µL PI was added in an ice bath, followed by immediate analysis by flow cytometry Cell Quest software (BD Biosciences).	48 hrs at base line