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Clinical Trial Details — Status: Completed

Administrative data

NCT number NCT01574404
Other study ID # 0911010734
Secondary ID
Status Completed
Phase N/A
First received March 16, 2012
Last updated June 10, 2015
Start date September 2011
Est. completion date December 2014

Study information

Verified date June 2015
Source Weill Medical College of Cornell University
Contact n/a
Is FDA regulated No
Health authority United States: Institutional Review Board
Study type Interventional

Clinical Trial Summary

People with infertility undergoing in vitro fertilization (IVF) can test the embryos using a method called preimplantation genetic screening (PGS) before they are implanted in the uterus to possibly increase their chances of having a successful pregnancy. One or more cells are removed from the embryo. The chromosomes inside the cells are then tested to identify normal or aneuploid embryo(s).

The investigators propose to evaluate a test called micro array analysis on the chromosomes of the first polar body. This method tests part of the egg that would normally be lost and may help us choose the embryo most likely to become a healthy baby.


Description:

The polar body (PB) technique makes use of the 23 chromosomes contained in the first polar body, a part of the egg that is extruded at ovulation. Normally the first polar body disintegrates. However, it can be salvaged by polar body biopsy prior to this so that the chromosomes within can be analyzed. PB has three distinct advantages over conventional Fluorescent in-situ Hybridization (FISH) analysis. First, the polar body biopsy is performed on the day that the oocyte is retrieved (day 0) rather than day 3 so results are available earlier. Secondly, this methodology does not remove cells from the growing embryo. The polar body biopsy removes genetic material that would otherwise degrade. Finally, microarray analysis evaluates all 23 chromosomes while only 9-10 chromosomes can be studied with FISH analysis.

The investigators propose to perform microarray analysis in 50 IVF/ICSI (intracytoplasmic sperm injection) patients undergoing PGS.

Day 0 ICSI; PB* Day 1 Day 2 Day 3 Embryo biopsy with FISH or Microarray analysis Day 4 Day 5 Embryo transfer or embryo biopsy with Microarray analysis followed by Embryo Cryopreservation

Polar body biopsies will be frozen and later analyzed, The PB results will remain undisclosed until the end of the study period. PB results will not be used to guide treatment or clinical management. Embryos will be chosen or deselected for transfer solely based on the standard of care treatment aneuploidy screening analysis.

The three specific aims of the project are as follows:

- To determine the efficacy of PB biopsy and analysis by comparing the PB results to the Fluorescent in-situ Hybridization (FISH)/microarray results

- To evaluate the safety of PB by comparing the implantation , miscarriage, pregnancy, and delivery rates

- To determine the error rate of FISH analysis by analyzing the remaining cells in embryos determined to be abnormal by initial FISH/microarray analysis


Recruitment information / eligibility

Status Completed
Enrollment 33
Est. completion date December 2014
Est. primary completion date July 2012
Accepts healthy volunteers Accepts Healthy Volunteers
Gender Both
Age group 18 Years to 42 Years
Eligibility Inclusion Criteria:

1. Are undergoing IVF/Intracytoplasmic sperm injection(ICSI)

2. Are considering preimplantation genetic screening for aneuploidy

3. Are between ages 18 and 42 inclusive (female partner)

Exclusion Criteria:

1. Using sperm from a testicular source

2. Fewer than 10 oocytes retrieved

3. Fewer than 6 oocytes fertilized normally

4. Complete fertilization failure (no oocytes fertilize)

Study Design

Endpoint Classification: Safety/Efficacy Study, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Diagnostic


Related Conditions & MeSH terms


Intervention

Procedure:
Polar body biopsy with PGS
PB biopsy and analysis by comparing the PB results to the FISH/microarray results

Locations

Country Name City State
United States Ronald O. Perelmand and Claudia Cohen Center for Reproductive Medicine New York New York

Sponsors (1)

Lead Sponsor Collaborator
Weill Medical College of Cornell University

Country where clinical trial is conducted

United States, 

Outcome

Type Measure Description Time frame Safety issue
Primary Efficacy of polar biopsy and preimplantation genetic analysis To determine the efficacy of PB biopsy and analysis by comparing the PB results to the FISH/microarray results 1 year No
Secondary Safety of polar biopsy To evaluate the safety of polar biopsy by comparing the implantation, miscarriage, pregnancy, and delivery rates 1 year Yes
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