Anaplastic Glioma Clinical Trial
Official title:
Hypoxia Diagnosis and Evaluation Using F-MISO PET and Biomarkers in Brain Tumors
Background :
In malignant gliomas, hypoxia is associated with tumour angiogenesis and tumour progression.
This multidisciplinary preclinical and clinical project aims to validate the use of
18F-FMISO as a hypoxic marker for diagnosis, treatment and follow-up of malignant gliomas.
Indeed, non-invasive methods of imagery such as Positron Emission Tomography (PET) and
biological methods (after surgical resection) to detect the endogenous expression of factors
induced by the hypoxia would allow to identify hypoxic areas. Identifying, with accuracy,
the hypoxic areas could allow the clinicians to evaluate the response to the anti-angiogenic
agents (preliminary validation in the preclinical project) and to optimize the combination
of the anti-VEGF treatments with other conventional therapeutic approaches (radiotherapy,
chemotherapy or other molecules).
Research project :
This research project includes 3 steps : first the investigators will establish the
18F-FMISO production technique for clinical application at CYCERON PET center. The second
step consists in the preclinical validation of 18F-FMISO as a hypoxic marker and as a
powerful tool for evaluating the therapeutical efficiency of anti-angiogenic treatment
(sutent) in experimental rat gliomas. The third step is the clinical trial HypOnco. This
research proposal aims to develop and use non invasive imaging methods (18F-FMISO with PET)
and biological methods (after surgical resection) to detect hypoxic (HIF1 and HIF2) and
angiogenic (VEGF and EPO) regions in different malignant gliomas with different degrees of
vascularization (15 patients with grade III gliomas and 15 patients with grade IV
glioblastomas). Within the same patient 18F-FMISO as a hypoxic index will be performed. A
magnetic resonance imaging examination (MRI) including perfusion sequences and MR
spectroscopy will also be assessed for each patient. Following this imaging protocol,
surgical resection will be performed allowing us to study expression of endogenous factors
induced by hypoxia and angiogenic factors by real time RT-PCR and in immunohistochemistry.
The data obtained will enable us to establish :
- a hypoxic index (18F-FMISO with PET).
- an index of hypoxic factor expression (HIFs)
- an angiogenic index (VEGF, EPO, vascular markers)
The investigators will characterize the links between these data and also with the following
parameters:
- clinical (age, Karnofsky performance status, survival)
- MR parameters included perfusion and spectroscopy
- histological (necrosis, cellular proliferation, atypical cell abnormalities,
vascularization).
Expected results and clinical advantages
- To establish the 18F-FMISO production technique
- To propose the 18F-FMISO as a non-invasive marker for efficacy of antiangiogenic
treatment in a preclinical study.
- To define the relationship between the 18F-FMISO uptake and tumour grade, patient
survival, tumour recurrence, expression of hypoxic and angiogenic factors and tumour
vascularisation.
- To provide a hypoxic index in cerebral tumours from 18F-FMISO PET, allowing diagnosis
and prognosis improvement for optimal treatment orientation and strategy.
In the field of the clinical applications, this tool will allow to :
- Optimize radiotherapy treatment by identifying with accuracy, using 18F-FMISO PET, the
most hypoxic areas which are also the most radio resistant.
- Evaluate antiangiogenic therapy efficacy
n/a
Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Diagnostic
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