G-CSF Treatment for Amyotrophic Lateral Sclerosis: A RCT Study Assessing Clinical Response

Amyotrophic Lateral Sclerosis Clinical Trial

Official title:

Granulocyte-Colony Stimulating Factor Treatment for Amyotrophic Lateral Sclerosis: A Randomized Control Trial Study Assessing Clinical Response

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT00397423
• Other study ID #: R01-123456-1
• Status: Completed
• Phase: Phase 2
• First received: November 8, 2006
• Last updated: November 27, 2007
• Start date: December 2006
• Est. completion date: August 2007

Study information

• Verified date: November 2007
• Source: Peking University
• Contact: n/a
• Is FDA regulated: No
• Health authority: China: Food and Drug Administration
• Study type: Interventional

Clinical Trial Summary

This study will examine the effectiveness of G-CSF in treating patients with amyotrophic lateral sclerosis (ALS) - a fatal neurological degenerative disease that causes adult-onset, progressive motor neurons loss in the spinal cord, brain stem and motor cortex. Patients develop progressive wasting and weakness of both upper and lower limbs, bulbar and respiratory muscles. Usually death from respiratory failure typically is within 3-5 years of diagnosis. Although there are various treatments for ALS, riluzole is the only approved treatment to delay the disease progression. G-CSF is an approved drug that is used to increase white blood cell counts. Moreover, G-CSF and its receptor are expressed by neurons. It acts anti-apoptosis by activating several protective pathways, stimulates neuronal differentiation of adult neural stem cells in the brain, and improves long-term recovery. G-CSF is a novel neurotrophic factor, and a highly attractive candidate for the treatment of neurodegenerative conditions such as ALS.

Patients 18 to 65 years of age who have had mild to moderately severe ALS for 0.5 to 2 years of duration may be eligible for this study. Candidates will be screened with a medical history and possible review of medical records, physical examination, blood test, urine and stool analyses, electrocardiogram, electrophysiological examination, neurological imaging and, for women, a pregnancy test.

Participants will have drug therapy according to randomized number. One group receives G-CSF while other group receives placebo. All of the participants receive riluzole treatment. For the procedure, patients are given a medication to lessen anxiety and any discomfort. Patients receive drug injections every 3 months for 5 days. The G-CSF dosage is 5μg/kg/day. Physical examination and interview, Appel ALS scale and ALS-Functional Rating Scale will be done in 14, 28 days and 3, 6, 9, 12 months. Electrophysiological examination will be tested per 3 months. Blood samples will be collected on treat 5, 14, 28 days and 3, 6, 9, 12 months.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 40
• Est. completion date: August 2007
• Accepts healthy volunteers: No
• Gender: Both
• Age group: 18 Years to 65 Years

Eligibility

Inclusion Criteria:

• All subjects must have a verifiable diagnosis of ALS of 0.5 to 2 years' duration. The diagnosis must be supported by the Revised Criteria of the World Federation of Neurology. The grades of diagnosis must be clinically definite ALS or clinically probable ALS.

• All subjects must be over age 18 and below 65.

• The ALS is mildly to moderate based on ALS Health State Scale.

• Electrophysiological features show CMAP amplitude of motor nerve normal or mild declining.

• Serum creatine kinase is normal or mild upper, less than 500U/L.

Exclusion Criteria:

• If anyone of the above eligibility requirements is not met

• Use of any other investigational agent within 30 days beginning the treatment phase of this study

• Severe cardiac, pulmonary, hepatic or/and hematic disease

• HIV positivity or signs and symptoms consistent with HIV infection

• Pregnant or nursing women

• History of cancer with less than 5 years documentation of a disease-free state

• History of anaphylactic reaction or hypersensitivity to G-CSF or proteins derived from E.coli

• Alcohol or drug abuse in recent 1 year

• Can't understand or obey the rules of treatment

• Blood donor in recent 30 days

Study Design

Allocation: Randomized, Endpoint Classification: Safety/Efficacy Study, Intervention Model: Parallel Assignment, Masking: Double Blind (Subject, Caregiver, Outcomes Assessor), Primary Purpose: Treatment

Related Conditions & MeSH terms

• Amyotrophic Lateral Sclerosis
• Motor Neuron Disease
• Sclerosis

Intervention

Drug:
• Granulocyte Colony Stimulating Factor
Granulocyte Colony Stimulating Factor,5ug/kg/day iH Qd*5 days,3 months interval for 4 times
• NS
NS 1ml iH Qd*5 days, 3 months interval for 4 times

Locations

Country	Name	City	State
China	Dongsheng Fan	Beijing	Beijing

Sponsors (1)

Lead Sponsor	Collaborator
Peking University

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	the mean rate of decline of ALS-FRS score		12 months
Secondary	the mean rate decline of the AARS score		12 months