Clinical Trial Details
— Status: Completed
Administrative data
| NCT number |
NCT04878237 |
| Other study ID # |
MUViennaAstra2021 |
| Secondary ID |
|
| Status |
Completed |
| Phase |
N/A
|
| First received |
|
| Last updated |
|
| Start date |
March 25, 2021 |
| Est. completion date |
May 31, 2021 |
Study information
| Verified date |
August 2021 |
| Source |
Medical University of Vienna |
| Contact |
n/a |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Interventional
|
Clinical Trial Summary
IgE-associated allergy is a hypersensitivity disease affecting more than 40% of the
population in industrialised countries. Recently the kinetics of change of clinical and
immunological parameters (e.g. nasal blockage and cytokine profiles) in response to allergen
exposure have been described. Additionally through recent placebo controlled studies it has
become clear that the response of certain cytokines can not only be triggered by allergen
exposure but also mechanically e.g through the insertion of nasal swabs for collection of
cytokines. However it is not clear to what extent the mechanically triggered cytokine
responses may differ between healthy, allergic and asthmatic patients who have been shown to
have different cytokine profiles in their nasal secretions and varying impairment of their
respiratory epithelium. As collection devices for nasal secretions are frequently used in
clinical studies, the investigators aim to assess the impact of mechanical stimulation by
frequent cytokine sampling on the cytokine profile.
Description:
Allergic rhinitis (AR) and allergic asthma are a common manifestations of Type I allergy, an
IgE-mediated hypersensitivity disease estimated to affect as much as 40% of the global
population . IgE is produced by B cells and binds to the surface of mast cells and basophils
via its high affinity Fce receptor I (FceRI). Crosslinking of surface IgE by its specific
allergen induces degranulation of the cells as well as release of inflammatory mediators.
This process which develops over minutes leads to the typical ocular, nasal and pulmonary
symptoms of AR and asthma such as itching, reddening of the eyes, runny and/or congested nose
and wheezing due to air trapping as a result of lower airway obstruction. Some patients may
also develop late phase reactions (LPR). These responses typically develop 2-6 hours after
allergen exposure, peaking in intensity between 6-9 hours and are mostly characterised by
tissue oedema which manifests as airflow obstruction in the airways.
Since the introduction of easy to use and highly absorptive mucosal fluid sampling devices
the mediators involved in the immediate and late phase allergic responses and the kinetics of
their release have become well characterised over the past few years. Efforts by Imperial
College London and unpublished data from the investigators group in Vienna have shown that
key mediators involved in the immediate phase include mast cell derived mediators, (Tryptase,
PGD2), epithelial alarmins (IL-33, sST2), and complement (C3a, C4a, C5a, D-Dimer). Mediators
involved in the late phase reactions also include alarmins (sST2, TSLP), Th2 cytokines (IL-4,
IL-5, IL-13), neutrophil mediators (MMP-9) and eosinophil mediators (EDN).
However what has also recently become clear through placebo controlled studies is that some
mediators are mechanically mediated (unpublished observations), being released into the
extracellular environment as a result of the sampling process itself. This is of vital
interest as understanding which cytokines are mechanical and which are allergen specific will
help guide future studies attempting to target specific biomarkers for treatment or disease
monitoring. In order to understand if the mechanical release plays any role in the
pathological response to allergens different patient cohorts will need to be investigated to
see if their responses differ in any way.
Rational of the Study
In light of this the investigators propose a study to investigate the effects of mucosal
sampling on cytokine release from nasal epithelium. The investigators plan to sample
different patient cohorts (allergic rhinitis, allergic asthma, non-allergic) to see how
mechanical effects are influenced by sampling and allergic or non-allergic status. By
including 10 patients in each group the investigators hope to establish a range of "normal"
for mechanical release of allergen relevant cytokines in each study group which will help
inform future studies investigating allergen specific responses. In order to map both the
symptomatic and objective responses to airway sampling, symptom scores and airway flow meters
will be employed. To map the kinetics of responses the participants will undergo a mucosal
sampling time course over the course of an 8 hour period on a single day. As no
interventional substances are being given, this study will carry very low risk to the
individuals involved while shedding important light on the epithelial responses to mechanical
manipulation.
Study design In this single-center, pilot study a total of 36 patients (n=12 per group) will
be recruited on a voluntary basis and will all receive nasal sampling. Study participants
will undergo the nasal sampling in a single visit.
Screening Visit
Procedure:
- Signing of the Informed Consent Form
- Allocation of screening number
- Demographic Data
- History of allergy
- Assessment of In- and Exclusion Criteria
- Pregnancy test for females if requiring SPT
- Medical history
- Patients reporting a history of asthma will have to provide objective evidence of airway
obstruction and reversibility (Spirometry reversibility or PC20 methacholine/histamine
<8mg)
- Concomitant medication
- Anterior rhinoscopy
- Standard SPT with commercial extract from: hazel, ash, grass mix, ragweed, mugwort, D.
pteronyssinus, D. farina, cat and dog dander, alternaria, cladosporium, histamine and
negative control
- Blood sampling (max 60ml of heparinized blood and serum) - A part of the blood sample
will be directly sent to the laboratory for analysis of total IgE, and specific IgE to
relevant allergens. From the rest of the blood, RNA will be prepared and stored at -80°C
and serum will be separated and stored at -20°C.
- nasal sampling (mucosal curette sampling and nasal secretion sampling)
Sampling Visit
Procedure:
- Reassessment of In and Exclusion Criteria (allergen-specific IgE results)
- Recording of concomitant medication
- Baseline Blood sampling
- Baseline nasal specimen collection for both nostrils (nasal secretion sampling) at 0min
- Baseline TNSS score
- Baseline PNIF
- Adverse event (AE) recording
- Blood, PNIF, nasal sampling (both nostrils: mucosal curette sampling and nasal secretion
sampling), and TNSS score, 15min, 30min, 45min, 60min, 75min, 90min, 120min, 180min,
240min, 300min, 360min, 420min, 480min after onset of sampling timecourse
4 Study procedures
4.1 Medical history of the participants, demographic data, concomitant medication The
participants will be asked for their medical history including demographic data and
concomitant medication, their allergic symptoms including intensity and duration and their
allergic medication history.
4.2 History of allergy and TNSS score The participants will be asked for occurrence of
sneezing, rhinorrhoea, nasal pruritus, nasal congestion and to grade each symptom. They will
use the following symptom score method: 0 = absent; 1 = mild - present but easily tolerated;
2 = moderate - present, symptom is bothersome but not interfering with daily life activities;
3 = severe - symptom is difficult to tolerate and is interfering with daily life activities.
4.3 Anterior rhinoscopy Anterior rhinoscopy will be performed once during the screening visit
to exclude anatomical variances (e.g. septal deviation) or pathological changes (e.g. nasal
polyps). It will be performed by using a speculum with the patient seated with the head
slightly back.
4.4 Peak Inspiratory Nasal Flow Meter (PNIF) The portability and ease of the PNIF provides a
unique opportunity to obtain objective measurements. It has also recently been validated as a
study tool during nasal allergen challenge.
4.4.1 Procedure: The PNIF uses a variable diameter tube calibrated directly in litres per
minute along with a low inertia indicator ring. The position of the ring after an inspiratory
manoeuvre indicates the maximum flow achieved. The device works when a patient inhales
through the mouth or nose, this causes air to be drawn through the meter and a cursor moves
along the scale to indicate the speed of inhalation. The flow rate achieved can be noted by
checking the position of the cursor against the calibrated scale.
4.4.2 PNIF in study conduct: The participants will be trained how to use the PNIF on the day
of the study and then it will be used at all study timepoints to gain information on nasal
flow.
4.5 Skin prick test 4.5.1 Procedure: Allergens are inserted into the dermis of the forearm by
gently pricking the skin through a drop (20ul) of an allergen-containing solution with a
sterile lancet with at least 2 cm between each individual application point. 0.9% sodium
chloride solution will be used as negative and histamine as positive control. In previously
sensitized individuals, itchy swelling and a reddening of the skin (wheal and flare reaction)
will occur within 15 minutes upon challenge with the respective allergen. After 20 minutes,
allergen solutions will be wiped off and margins of the wheals will be traced with a ball
point pen. Transparent tape will be bonded on the skin in order to transfer the outline of
the wheal to the tape for the records. The surface of the wheals will be calculated by
digital planimetry. For inclusion, wheals of at least 3 mm in diameter will be regarded as
positive reactions.
4.5.2 Test solutions: A commercially available panel of tree and grass pollen, weed and
perennial allergens (Allergopharma, Vienna, Austria) will be used at the screening visit and
will be stored according to the manufacturer's instructions. As controls, sterile 0.9% NaCl
solution (negative control) and histamine (positive control, Allergopharma, Vienna, Austria)
will be used. They will be stored according to the manufacturer's instructions.
4.5.3 SPT in study conduct:
Screening Visit:
A standard skin prick test with commercial birch pollen extract and a panel of tree and grass
pollen, weed and perennial allergens (all Allergopharma, Vienna, Austria) as used in routine
diagnostic in the allergy clinic of the ENT department will be performed to assess the
participants sensitization profile at the screening visit.
4.5.4 Safety precautions As a safety precaution, the participants will be monitored for 30
minutes after testing. All investigations will be done in the Outpatient Clinic of the
ENT-Department, where full emergency equipment is available.
4.6 Blood sampling and measuring of immunological parameters Blood samples will be taken at
the screening visit and at all study visits by puncture of the antecubital vein before skin
testing. A maximum of 60ml per visit will be taken either heparinized (e.g. for basophil
sensitivity assays) or for the preparation of serum (e.g. for assessment of total and
allergen specific immunoglobulin levels or RBL assay). Serum vials will be processed and
serum will be stored at -20°C. Allergen-specific IgE of blood samples of the screening visit
will be measured by CAP-FEIA to assess the allergen-specific RAST-class of the patients.
Measurement of total, free, and allergen-specific serum IgE, IgG, IgA and IgM will be
performed either by CAP-FEIA, by microarray measurement or ELISA. Immune mediators will be
measured using the MSD platform either by us or by our collaborators at AstraZeneca,
Cambridge, UK. The measurement will be performed at the end of the study in all collected
serum samples to minimize interassay variation.
4.7 Pregnancy testing In female participants, pregnancy will be excluded with a standard
urine pregnancy test. The test will be performed before the first skin prick test at the
screening visit and afterwards once a month.
4.8 Nasal specimen collection Nasal specimen collection will be performed at all visits. For
every visit specimens will be collected using nasosorption FXi/PU (containing a synthetic
absorptive matrix (SAM)), or a 10 cm-long plastic curette (nasosorption devices from Hunt
Developments, UK) (Curette devices from Arlington Scientific, USA)
4.8.1 Nasosorption using FXi/PU (Hunt Developments, UK). Under visualization, the device will
be inserted into the nasal cavity and be placed along the lateral wall against the inferior
turbinate. The index finger of the patient will be used to press onto the external aspects of
the alar and lateral nasal cartilages to hold the device in place. After 1 minute, the
devices will be removed and then frozen at -80°C.
4.8.2 Mucosal mRNA sampling A 10cm nasal curette (either Rhino-Probe, Arlington Scientific,
USA or Cellskim, Hunt Developments, UK) will be used. Under direct visualisation the curette
will be brought to lie against the mid-inferior portion of the inferior turbinate. The
curette will be pressed against the mucosal surface moved outwards 2-3 times. This motion
will be repeated 2-3 times to ensure good sample collection. This curette and technique have
been shown to cause no significant discomfort to participants and thus it has the advantage
of no requirement for local anaesthetic. After collection Cells will be lysed in Qiagen RLT
buffer and stored at -80°C for later mRNA analysis.
5 Investigational products and study medication 5.3 Concomitant medication 5.3.1 General All
additional medication being taken by the subjects on entry to the study or at any time during
the study are regarded as concomitant medication and will be documented in the CRF.
Concomitant medications should be kept to a minimum during the study, but if considered
necessary may be given if not interfering with the study protocol.
5.3.2 Rescue medication Although anaphylactic events are very unlikely in this study they
always have to be considered possible. Therefore a complete emergency kit will be available
immediately in case of anaphylactic reactions at SPT. Furthermore if the participant is
suffering from strong skin symptoms after the SPT desloratadin 5mg will be provided to
ameliorate the symptoms.
5.3.3 Prohibited concomitant medication
Subjects must remain off medications interfering with study procedures for the duration of
the study. The following concomitant treatments are not permitted during this study:
- Systemic or topical corticosteroids
- Other immunosuppressant drugs
- Antihistamines or disodium cromoglycate 3 days prior to screening visit
- Systemic or intranasal adrenergic drugs
- Psychopharmacological drugs
- ACE-inhibitors or beta-blockers
6 Adverse events An adverse event is any event during a clinical study, including
intercurrent illness or accident, which impairs the well-being of the subject; it may
also take the form of an abnormal laboratory value. The term adverse event does not
imply a causal relationship with the study treatment.
All subjects experiencing adverse events - whether considered associated with the use of the
study treatment or not - will be monitored until symptoms subside and any abnormal laboratory
value has returned to baseline, or until there is a satisfactory explanation for the changes
observed, or until death, in which case a full pathology report from a qualified pathologist
will be provided. All findings must be reported on an "adverse event" page in the case record
form.
All adverse events will be reported on and documented as described below. Adverse events are
divided into the categories "serious" and "non-serious". This determines the procedure which
must be used to report/document the adverse event (see below).
6.1 Definition of serious and non-serious adverse events
A serious adverse event is:
- Any event that is fatal or life-threatening
- Any event that is permanently disabling
- Any event that requires or prolongs hospitalization
- Any event that involves cancer, congenital abnormality, or occurs as a result of
overdose (application of more than the stipulated dose).
Adverse events which do not fall into these categories are defined as non-serious.
6.2 Assessment of severity of AE
Regardless of the classification of an adverse event as serious or non-serious (see above),
its severity must be assessed as mild, moderate or severe, according to medical criteria
alone:
Mild = does not interfere with routine activities, acceptable Moderate = interferes with
routine activities Severe = impossible to perform routine activities, considered as
unacceptable by the physician, requires treatment, requires discontinuation of study, or has
residual effect.
It should be noted that a severe adverse event need not be serious in nature. Regardless of
severity, all serious adverse events must be reported on as below.
6.3 Reporting/documentation of adverse events Adverse events are collected by spontaneous
reporting. 6.3.1 Reporting/documentation of serious adverse events All serious adverse events
which occur during this study whether considered to be associated with the study or not, must
be documented on an "Adverse event" page in the case record form.
A follow-up report including all new information obtained on the serious event must be
prepared and will be collected.
The investigator will submit on request copies of all these reports to the ethics committee.
Where necessary, investigators will inform the authorities.
6.3.2 Reporting/documentation of non-serious adverse events These are to be documented on an
"Adverse event" page in the case record form.
7 Statistical analysis These analyses will seek to explore any statistically significant
differences in longitudinal nasal cytokine trajectories between healthy individuals, atopic
individuals and asthmatics. We will first undertake-basic descriptive statistics. Subject
characteristics (e.g. health status, demographic variables) and outcome variables (e.g. nasal
cytokine release, clinical responses) will be described by calculating maximum, minimum,
median, mean and standard deviation for metric variables and absolute and relative
frequencies for categorical variables for each subject group (healthy individuals, atopic
individuals and asthmatics).
Log-changes from baseline will be compared between groups, and at each time point using
t-tests for each pairwise comparison between groups, to explore the
shape-of-trajectories-over-time. For graphical illustration, the individual
log-baseline-change-trajectories of each patient will be plotted as well as group means with
95% CIs.
If log-changes-from-baseline-exhibit reasonable-linearity, we will test for an association
between subject groups and longitudinal trajectories using mixed linear models with
subject-specific random intercepts to account for intra-individual correlations of repeated
measures, and group by time-interaction terms to test for statistically significant
differences in trajectories. The motivation of this mixed modelling approach will be to
increase-power-by-combining information-across multiple-time-points. Mean trajectories and
95% CIs as inferred by the mixed modelling work will also be plotted
8 Ethical and legal aspects The study will be carried out in keeping with local legal
requirements and GCP. It will be performed in accordance with the guidelines of the
Declaration of Helsinki (1964), including current revisions.
8.1 Informed consent of subject Before being admitted to the study, the subject must have
consented to participate after the nature, scope and possible consequences of the clinical
study have been explained in a form understandable to him/her. The subject must give consent
in writing. The signature of the investigator will confirm the subject's consent. The patient
may withdraw the consent, even without giving comments, at any time and without negative
consequences for his future medical care. Nasosorption and/or RNA and/or blood samples may be
sent in an anonymized form to collaboration partners outside Austria for analysis (e.g.
analysis of cleaved and whole IL-33 by AstraZeneca, Cambridge UK). The patient is informed
about this in the informed consent form.
8.2 Acknowledgement/approval of the study Before the start of the study, the study protocol
will be submitted to the Ethics committee of the medical university Vienna and the general
hospital of Vienna (Borschkegasse 8b/E 06, 1090 Vienna, Austria).
8.3 Insurance All subjects participating in this study will be insured at the Zürich
Insurance company (Zürich-Versicherungs-Aktiengesellschaft, Schwarzenbergplatz 15, 1010
Vienna, Tel. +43 50 1255 1255).
8.4 Confidentiality All subjects' names will be kept secret in the investigators files.
Subjects will be identified throughout documentation and evaluation by the number allotted to
them during the study. The subjects will be told that all study findings will be stored and
handled in strictest confidence.
9 Documentation and use of study findings 9.1 Documentation of study findings All results
collected during the study will be collected in a source data file and will later be
transferred to the case report forms (CRFs). All entries on the case record forms will be
made legibly in black or blue ink. If corrections are made to entries in the case record
form, the words or figures will be ringed and a single stroke drawn through them. The correct
value will be entered beside the old entry and date and the correction will be initialled.
Incorrect entries must not be covered with correcting fluid or obliterated or made illegible
in any way. The completed CRFs will be signed by the investigator. CRFs will be completed
immediately after the final examination. The medical records upon which the CRF is based will
be kept for at least 15 years.
9.2 Use of the study findings The findings of this study will be published by the
investigators in a scientific journal and presented in scientific meetings. The manuscript
will be circulated to all co-investigators before submission.
10 Considerations on Potential Risks and Benefits for Study Participants and selection of
dose
The included participants will not benefit directly from the study. However as the kinetics
of mechanical mucosal stimulation is still not completely understood this comprehensive pilot
study will provide an opportunity to guide future research which will hopefully lead to the
discovery of novel therapeutic targets.
Potential risks for the participants include pain, infection and hematoma at the site of
blood draw. During nasal mucosa and secretion sampling light nasal bleeding is a possible
risk. During SPT, although highly unlikely, anaphylactic reactions are a possibility. To
minimize risk of anaphylactic reactions and to provide optimal treatment in case an
anaphylactic reaction should occur, patients will be monitored for a minimum of 30 minutes
after SPT. At all participant visits trained personnel and emergency medication will be
available all the time.
