Age Factors Clinical Trial
Official title:
Co-culture Versus Sequential Culture System in Advanced Maternal Age.
Fertilization and embryo development in vivo occurs in appropriate conditions in the
mammalian reproductive tract. However in assisted reproductive technology (ART) embryos and
oocytes are exposed to certain factors such as temperature, O2 pressure and pH fluctuations
that may affect embryo development and viability in vitro. In Preimplantation Genetic
Screening program (PGS) the investigators need to culture the embryos until day 5 so the
requirement of culture techniques must be optimal to produce acceptable blastocyst rates. As
it has been shown before, embryo culture conditions have the potential to impact biopsied
embryos and hence PGS success rates.
Nowadays the coculture system with endometrial cells could be an alternative option to
improve the blastocyst rates since some publications have indicated that endometrial cells
may provide trace elements and growth factors that are not present in defined culture media
and reproduce physiologic conditions. Although the role of coculture in vitro fertilization
(IVF) has been controversial, a recent meta-analysis has established the potential benefits
of coculture system.
The aim of this study was to compare embryologic and clinical outcomes in terms of
blastocyst, implantation and pregnancy rates to evaluate endometrial epithelial cell (EEC)
coculture versus sequential culture system in advanced maternal age ( ≥39 years) in the PGS
program.
n/a
Observational Model: Case Control, Time Perspective: Retrospective
| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Completed |
NCT01567020 -
Central Auditory Processing Deficits Associated With Blast Exposure
|