LK101 Combined With PD-1 or PD-L1 Monoclonal Antibody in the Treatment of Lung Cancer

Advanced Lung Carcinoma Clinical Trial

Official title:

A Study of LK101 Combined With PD-1 or PD-L1 Monoclonal Antibody in the Treatment of Lung Cancer to Evaluate the Safety, Tolerability and Preliminary Efficacy

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT05886439
• Other study ID #: LK101-IIT02
• Status: Recruiting
• Phase: Phase 1
• Start date: May 11, 2023
• Est. completion date: December 30, 2026

Study information

• Verified date: April 2023
• Source: Cancer Institute and Hospital, Chinese Academy of Medical Sciences
• Contact: Jie Wang, MD,PhD
• Phone: 13910704669
• Email: jiewang_hr[@]sina.com
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This is a open lable, single-center phase Ib/IIa study for patients with local advanced or metastastic NSCLC or ES-SCLC, who failed with previous anti-PD-1/PD-L1 therapy. The aim is to observe and evaluate the safety, tolerability and efficacy of LK101 injection combined with pembrolizumab or durvalumab respectively in the incurable NSCLC and SCLC.

Description:

This study is designed to evaluate the safety and efficacy of LK101 injection combined with pembrolizumab or durvalumab, which devided into 2 cohorts: cohort 1: patients with locally advanced or metastastic (stage IIIB-IV) NSCLC who has progressed/relapsed after anti-PD-1/PD-L1 therapy. eligible subjects will receive LK101 injection and pembrolizumab treatment. cohort 2: patients with extensive SCLC who failed with at least first-line standard therapy with PD-L1. eligible subjects will receive LK101 injection and durvalumab treatment. LK101 will be administered in a prime-boost schedule of 4 priming vaccination followed by 3 booster vaccinations. For the priming phase: LK101 administered once a week at Days 1, 8, 15, 22. For the booster phase: total of 3 vaccinations will be given, Q3W from the end of priming dose. Treatment can be continued according to the investigator's evaluation, subsequent treatment is administered Q6W. Patients will receive a combination of pembrolizumab(200mg IV) Q3W and durvalumab (1500mg IV) Q3W in NSCLC and SCLC,respectively, until disease progression (PD), intolerable toxicity.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 40
• Est. completion date: December 30, 2026
• Est. primary completion date: July 30, 2025
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• signed informed consent;
• =18years, male or female;
• cohort1: Histologically/cytologically confirmed locally advanced or metastastic Non-small lung carcinoma (NSCLC), and received systemic treatment for recurrence/metastasis =3 lines; cohort2: Histologically/cytologically confirmed extensive small-cell lung carcinoma (ES-SCLC); Both cohorts required patients progressed/recurrenced after anti-PD-1/PD-L1treatment;
• Life expectancy of more than 3 months;
• Eastern Cooperative Oncology Group (ECOG) performance status of 0 - 1;
• At least one measurable lesion according to RECIST 1.1;
• The sequencing of tumor were qualified;
• According to the invistigators' judgment, venous vascular conditions can meet the needs of apheresis;
• For adequate organ function, the patients need to meet the following laboratory

indexes:

• hematologic functions(No blood transfusion or treatment with blood components and

without granulocyte colony stimulating factor in the past 14 days.):

• the absolute value of neutrophils (ANC) = 1.5x109/L;
• the platelet count was = 90x109/L;
• the hemoglobin > 9g/dL;
• Hepatic functions:
• Total bilirubin = 1.5 × normal upper limit (ULN); patients with liver metastasis allow = 3 × ULN;
• aspartate aminotransferase (AST) and alanine aminotransferase (ALT) = 2.5 × ULN (patients with liver metastasis allow ALT or AST = 5 × ULN);
• renal
• Serum creatinine = 1.5 × ULN and creatinine clearance (calculated by Cockcroft-Gault formula) = 50ml;
• patients with urinary protein = + + and confirmed 24-hour urinary protein quantity > 1.0g;
• Coagulation function is good, defined as international standardized ratio (INR) or prothrombin time (PT) = 1.5 times ULN;
• Normal thyroid function is defined as thyroid stimulating hormone (TSH) within the normal range. If the baseline TSH is beyond the normal range, subjects with total T3 (or FT3) and FT4 within the normal range can also be enrolled;
• FBG of patients without type 2 diabetes = 126 mg/dL or = 7.0 mmol/L, and that of patients with type 2 diabetes = 167 mg/dL or = 9.3 mmol/L; Or glycosylated hemoglobin (HbA1c) =8%;
• If there is a risk of pregnancy, all patient (male or female) are required to take appropriate methods for contraception during the study until the 6th month post the last administration of study drug;
• Well compliance, cooperate with follow-up;

Exclusion Criteria:

• History of hypersensitivity reaction to any vaccine and/or anti-PD-1/PD-L1 formulation ingredients; Or have had a previous severe allergic reaction to other monoclonal antibodies; Subjects who had previously discontinued anti-PD-1 /PD-L1 therapy due to "infusion reaction" or immune-related AE;
• Patients who have received therapeutic tumor vaccine products (including peptide vaccine, mRNA vaccine, DC vaccine, etc.);
• Diagnosis of malignant diseases other than study disease within 5 years before screening (except for malignant tumors that can be expected to recover after treatment);
• Patients received systemic antitumor treatment within 2 weeks before the apheresis, or receive reasearch drugs or device therapy;
• Received radiotherapy within 2 weeks prior to screening;
• Toxicity caused by previous treatment did not recover to CTCAE (version 5.0) Grade 1 or below (except hair loss and peripheral neuropathy);
• The tumor compresses the surrounding important organs or the superior vena cava, or invades the mediastinal great blood vessels, the heart, .etc;
• Patients who have recewived allogeneic hematopoietic stem cell transplantation or organ transplantation;
• A history of medical conditions that may trigger seizures (requiring treatment with antiepileptic medications);
• Patients who have active brain metastases or cancerous meningitis. Patients with treated brain metastases are eligible if they have been treated with brain metastases, and clinically stable for atleast 3 months, no evidence of disease progression 4 weeks before. All neurological symptoms had recovered, and off steroids at least 7 days prior to screening;
• Diaginosied or suspected of having an active autoimmune disease;
• patients with poorly controlled pleural effusion, pericardial effusion, or ascites requiring repeated drainage, or received pleural effusion or ascites treatment within the past 3 months;
• History of significant cardiovascular and cerebrovascular disease occurred in the 6

months prior to screening,Any of the following cardiac criteria:

• Mean resting corrected QT interval (QTc) > 470 ms;
• Left ventricular ejection fraction (LVEF) = 50%;
• American New York heart association (NYHA) heart function = 2 or higher;
• serious arrhythmia;
• poorly controlled hypertension;
• other serious heart disease;
• Patients with interstitial pneumonia, except those inactive and do not require hormone therapy disease;
• Patients diagnosed with active infections that are poorly controlled by systemic treatment;
• Any of the following test results are positive: human immunodeficiency virus (HIV) antibody, treponema pallidum antibody, hepatitis C virus (HCV) antibody, hepatitis B virus (HBV) surface antigen (HBsAg), HBV DNA and novel coronavirus nucleic acid;
• Active tuberculosis (TB) during screening;
• Treatment with systemic steroids or other immunosuppressive agents within 14 days prior to screening;
• Vaccination within 4 weeks prior to screening;
• Major injuries and/or surgery =< 4 weeks prior to screening;
• Persons with a history of psychotropic substance abuse and inability to abstain or with a history of mental disorders;
• Pregnant or lactating women;
• Skin diseases, such as psoriasis, may prevent intradermal vaccines from reaching the target area;
• Other conditions regimented at investigators' discretion.

Related Conditions & MeSH terms

• Advanced Lung Carcinoma
• Lung Neoplasms

Intervention

Drug:
• LK101 injection (personlized neoantigen pulsed DC vaccine )
LK101 will be administered in a prime-boost schedule of 4 priming vaccination followed by 3 booster vaccinations.
• Pembrolizumab
Patients will receive pembrolizumab(200mg IV) Q3W until disease progression (PD), intolerable toxicity.
• Durvalumab
Patients will receive durvalumab (1500mg IV) Q3W until disease progression (PD), intolerable toxicity.

Locations

Country	Name	City	State
China	Cancer hospital Chinese Academy of Medical Sciences	Beijing	Beijing

Sponsors (1)

Lead Sponsor	Collaborator
Cancer Institute and Hospital, Chinese Academy of Medical Sciences

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Tumor immune microenvironment before and after treatment	CD8+T cell, PD-1, PD-L1, etc.	24 months
Primary	DLT	incidence of Dose limited toxicity(DLT),incidence and severity of adverse events (AEs), serious adverse events (SAEs), and immune-related adverse events (irAEs); Clinically significant abnormal changes in laboratory tests and other tests.	Continuously throughout the study until 90 days after Termination of the treatment
Primary	AE	incidence and severity of adverse events	Continuously throughout the study until 90 days after Termination of the treatment
Primary	irAE	incidence and severity of immune-related adverse events	Continuously throughout the study until 90 days after Termination of the treatment
Primary	SAE	incidence and severity of serious adverse events	Continuously throughout the study until 90 days after Termination of the treatment
Secondary	ORR	Objective Response Rate (ORR)according to mRECIST 1.1 standard	accessed up to 24 months from baseline
Secondary	DoR	Duration of remission	24 months
Secondary	DCR	Disease Control Rate	24 months
Secondary	TTR	Time to remission	24 months
Secondary	TTP	Time to progression	24 months
Secondary	PFS	Progression Free Survival	24 months
Secondary	OS	Overall Survival	24 months
Secondary	Immune response evaluation	T cell response, cytokines, etc.	24 months