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Clinical Trial Details — Status: Completed

Administrative data

NCT number NCT01500941
Other study ID # 131/2010/77/2009/AP
Secondary ID
Status Completed
Phase Phase 3
First received December 21, 2011
Last updated January 3, 2012
Start date April 2010
Est. completion date September 2010

Study information

Verified date January 2012
Source Ospedale L. Sacco – Polo Universitario
Contact n/a
Is FDA regulated No
Health authority Italy: Ministry of Health
Study type Interventional

Clinical Trial Summary

Probiotics are suggested to have beneficial effects in atopic dermatitis (AD) treatment and prevention but their precise role is not yet clear.

The aim of this randomized double blinded active treatment vs placebo study was to evaluate clinical efficacy of intake of a combination of two probiotics (Lactobacillus salivarius LS01 and Bifidobacterium breve BR03) for the treatment of adult AD patients.

The rationale for the use of probiotics in the treatment of atopic dermatitis would be due to some experimental hypotheses:

1. The use of these microbial agents at an early age seems to play an important role in inducing immunity T type 1 (Th1) and inhibit the development of a Th2 response IgE mediated

2. the normal intestinal flora (including probiotics) would play an important role in inducing immunological tolerance

3. the hygiene hypothesis that the reduced bacterial environment would favour a type 2 response T and the development of allergic diseases


Recruitment information / eligibility

Status Completed
Enrollment 48
Est. completion date September 2010
Est. primary completion date September 2010
Accepts healthy volunteers Accepts Healthy Volunteers
Gender Both
Age group 18 Years to 55 Years
Eligibility Inclusion Criteria:Adults aged 18-55 years between

- Diagnosis of DA moderate and / or severe, as assessed by the use of a standardized index of severity of AD (SCORAD = Scoring Atopic Dermatitis index) (42), prepared by the European Task Force for atopic dermatitis

Exclusion Criteria:Allergic contact dermatitis (ACD), active

- In the absence of known food allergy elimination diet

- Chronic diseases (autoimmune diseases, COPD, heart disease, IRC, CNS disease, chronic gastrointestinal diseases, diabetes, congenital or acquired immunosuppression)

- Pregnancy and / or lactation

- Treatment with probiotics in the 6 months preceding enrollment

- Treatment with steroids and antihistamines systemically in the three months prior to enrollment

- Topical treatments with immunomodulators (tacrolimus or pimecrolimus) in the three months prior to enrollment

- Acute or chronic infectious diseases

- Pre-existing hypersensitivity to components contained in the probiotic

Study Design

Allocation: Randomized, Endpoint Classification: Safety/Efficacy Study, Intervention Model: Parallel Assignment, Masking: Double Blind (Subject, Investigator), Primary Purpose: Treatment


Intervention

Dietary Supplement:
probiotics
a mixture of Lactobacillus LS01 DSM 2275 and Bifidobacterium BR03 DSM 16604 at a dose of 1 x 109 colony forming units (CFU)/g each in maltodextrin
Other:
maltodextrin
sachets

Locations

Country Name City State
Italy Luigi Sacco Hospital Milano

Sponsors (1)

Lead Sponsor Collaborator
Ospedale L. Sacco – Polo Universitario

Country where clinical trial is conducted

Italy, 

Outcome

Type Measure Description Time frame Safety issue
Primary clinical effects of probiotics on adult atopic dermatitis To evaluate the clinical course of adult patients affected by atopic dermatitis after the intake of two probiotics vs placebo 20 weeks Yes
Secondary effects of probiotics on immune system and faecal microbiota in adult atopic dermatitis to evaluate these parameters: percentage of circulating Treg cells, percentage of Th17 cells, Th1 and Th2, percentage of Treg cells TLR2+, TLR4 + and TLR9 +, Quantization plasma LPS. Determination by gene amplification and culture of Lactobacillus salivarius and Bifidobacterium in the faeces of the two groups studied 20 weeks No