The Effect of Vitamin C on GCF Total Oxidant Capacity in Smoker Patients With Periodontitis

Vitamin C Deficiency Clinical Trial

Official title:

The Effect of Vitamin C Supplementation on GCF Total Oxidant Capacity in Smoker Patients With Periodontitis Following Non-surgical Periodontal Therapy. A Randomized Controlled Clinical Trial

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT05009355
• Other study ID #: 100200
• Status: Recruiting
• Phase: Early Phase 1
• Start date: January 11, 2019
• Est. completion date: December 21, 2022

Study information

• Verified date: March 2022
• Source: Cairo University
• Contact: abeer H Sharafuddin, PhD
• Phone: 00201284181671
• Email: abeer.hasan[@]dentistry.cu.edu.eg
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The effect of vitamin C supplementation on GCF total oxidant capacity in smoker patients with periodontitis following non-surgical periodontal therapy.

Description:

All subjects were screened by comprehensive periodontal examination and full periodontal charts were obtained and full-mouth periapical radiographs were taken using the long cone parallel technique at the time of the initial examination to confirm the diagnosis.

Both groups (smokers with stage III-IV grade B periodontitis) received full mouth scaling and root planing using ultrasonic scaler and hand instruments under local anesthesia, completed over 2 visits. Patients were given careful instruction in self performed plaque control measures: twice daily tooth brushing with a brush and tooth paste and once daily interdental cleaning using triangular wooden tooth picks and/or interdental brushes rinsing with 0.125% Chlorhexidine HCL mouthwash (Hexitol® ) to be used twice daily for 2 weeks. Group II received antioxidant vit C 2 grams / day for four weeks.

Collection of gingival crevicular fluid (GCF) sample:

In both groups, GCF samples were collected at the initial visit and collected again 3 months after non-surgical treatment as follows : at the selected area (the most periodontally affected site), all clinically detected supragingival plaque were removed carefully to minimize contamination of the paper strips by the plaque . The teeth selected were then gently washed with water, and the sites under study were isolated with cotton rolls to prevent contamination with saliva and gently dried with an air syringe . Filter paper strips were placed at the entrance of the crevice and were left in place for 30 secs ) and then placed in plastic eppendorf. Strips with any plaque, saliva, or blood contamination were discarded .The sample from the individual site was stored at -80°C for later processing . GCF was then extracted and assayed for the content of total oxidant capacity.

Quantitation of TOC in GCF:

GCF samples of both groups were assayed using ELISA technique for estimation of the level of total oxidant capacity (TOC) in GCF as follows:

The TOC was extracted from the paper strip by adding 100 μl of phosphate buffer saline to the eppendorf tube then mixed by vortex followed by centrifugation for 10 min at 3000Xg. The supernatant was used for estimation of TOC. For the quantitative determination of human TOC concentrations in GCF, PerOx (TOS/TOC) Immun-diagnostik Assay (ELISA) Kit was used. ELISA is intended for the quantitative determination of the total oxidative status/capacity (TOS/TOC) in Ethylene-diaminetetraacetic acid (EDTA)-plasma, serum and cell culture supernatants.

The determination of the peroxides is performed by the reaction of a peroxidase with peroxides in the sample followed by the conversion of TMB (Tetra-methylbenzidine) to a colored product. After addition of a stop solution the samples were measured at 450 nm in a microtiter plate reader. The quantification is performed by the delivered calibrator

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 40
• Est. completion date: December 21, 2022
• Est. primary completion date: September 30, 2021
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 60 Years

Eligibility

Inclusion Criteria:

• all patients were systemically healthy

• stage III-IV grade B periodontitis

• A minimum of two non-adjacent teeth having sites with = 5 mm interdental clinical attachment loss .

• Radiographic bone loss extending to middle third of root and beyond .

• All patients were heavy smokers i.e = 20 cigarettes / day .

Exclusion Criteria:

• patients who had received any periodontal or antibiotic treatment or vitamins supplementation during the last six months.

• pregnant or lactating women

• patients who refused to provide written informed consent

Related Conditions & MeSH terms

• Ascorbic Acid Deficiency
• Periodontitis
• Scurvy
• Vitamin C Deficiency

Intervention

Drug:
• Vitamin C
antioxidant

Locations

Country	Name	City	State
Egypt	Abeer Hassan Sharafuddin	Cairo

Sponsors (1)

Lead Sponsor	Collaborator
Cairo University

Country where clinical trial is conducted

Egypt, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Total Oxidant Capacity	unit	one month
Secondary	Gingival index	score (0 is good no bleeding 3 is worst spontaneous bleeding )	one month
Secondary	plaque index	score (0 is good no plaque 3 is the worst )	one month
Secondary	probing depth	mm	one month