Thrombosis Clinical Trial
Official title:
Human Umbilical Vein Endothelial Cells (HUVEC)-Perfusion
Evaluation of fibrin and platelet deposition on a human umbilical endothelial cell surface
in perfusion chamber experiments using human whole blood.
Open-label, non interventional study Perfusion chamber experiment will be performed in 30
healthy patients. The Impact of different pH-solutions on thrombus lysis will be evaluated
in an in-vitro second step
Outcome:
D-Dimer content of the thrombus reflecting the size of the thrombus.
All Subjects will have two perfusion chamber experiment as follow with an estimated maximum
of blood loss of 165ml:
Before blood perfusion, perfuse the system including the chambers with human umbilical vein
endothelial cell coated glass slides (HUVEC), with sodium chloride (NaCl) (0.9%), to ensure
no leaks and to remove all air bubbles.
Blood is drawn from a vein in the arm with a Surflo® Winged Infusion Set, 19G (Terumo
Europe, Leuven, Belgium) with a pump (Masterflex® L/S™, Cole-Parmer Instrument Company,
Vernon Hills, Illinois, USA). Five mL of blood is discarded before each perfusion.
Three in serial placed flow chambers (with HUVEC) heated to 37°C are used. They are made of
a Plexiglas block through which a cylindrical hole of 0.2 cm in diameter is machined. The
aorta pieces are perfused at 10 mL/min for 5 minutes, followed by a 30 seconds perfusion
with NaCl (0.9%).
The plasmin degraded thrombus D-Dimer content will be further evaluated under different
conditions (different pH solutions).
;
Observational Model: Case Control, Time Perspective: Prospective
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