| Eligibility |
Pre-screening registration
Inclusion Criteria:
1. Signed pre-screening Informed Consent Form
2. Patients with recurrent or metastatic solid tumor (any histology), who progress after
at least one standard therapy for advanced disease
3. Patient willingness and acceptance to participate in the immunology research is
mandatory
4. At least one lesion accessible to biopsy without putting patient at risk
5. Biopsies obtained previously (within a maximum of 12 weeks before pre-screening
visit), outside this protocol, for TIL assessment can be also considered for patient
selection. Fresh tumor biopsies for subsequent translational studies will be used as
baseline and collected at pre-screening (when biopsy will be performed for TIL status)
or at screening (when archival biopsy will be used for TIL status). Biopsy from
metastatic lymph nodes is accepted.
6. Eastern Cooperative Oncology Group (ECOG) clinical performance status: 0-1 for all
patients, independently of the number of previous lines of therapy.
7. Life expectancy of = 12 weeks
8. Patients with Glycose-6-Phosphate Dehydrogenase (G6PD) deficiency or any other
hereditary coagulation disorder are excluded, as well as patients with clinical
history of Reye syndrome
9. Adequate serology defined by the following laboratory results obtained during
pre-screening period (day-28 to day-14).
1. Seronegative for HIV infection (anti-HIV-1/-2)
2. Seronegative for hepatitis B infection (HBs Ag, total anti-hemoglobin C (HBc),
anti-HBs). Patients with past or resolved hepatitis B infection (defined as
having a negative hepatitis B surface antigen HBsAg test and a positive anti-HBc
antibody test) are eligible, if hepatitis B virus (HBV) DNA test is negative.
3. Seronegative for hepatitis C infection (anti-HCV): if a patient has positive
anti-HCV antibody, a negative hepatitis C virus (HCV) RNA need to be obtain to
register the patient.
Study registration
Inclusion Criteria
1. Signed main study Informed Consent Form
2. Absence of tumor-infiltrating intraepithelial Cluster of differentiation-8 positive T
cells (CD8+ T cells) by immunohistochemistry (IHC) on baseline biopsy defined as <5
CD8+ cells per high power field of tumor
3. At least one lesion accessible to biopsy without putting patient at risk
4. Number of metastatic lesions viewed on Positron emission tomography-computed
tomography (PET/CT) scan: for both Phase Ia and Phase Ib, the patient may have any
number of metastatic lesions. A maximum of 10 metastatic lesions visible by PET/CT
scan will be irradiated at the investigator's discretion. It is at the discretion of
the investigator to exclude a metastatic site from the radiation field that, due to
the radiotherapy volume, dose or location too close to a healthy organ, may put the
patient at risk of suffering from radiation induced toxicity. If this would be the
only metastatic site to be irradiated, the concerned patient will be excluded from the
study.
5. Absence of any psychological, familial, sociological or geographical condition
potentially hampering compliance with the study protocol
6. Adequate hematologic and end-organ function, defined by the following laboratory
results obtained within 14 days prior to the first study treatment:
1. Absolute neutrophil count = 1.5 g/L (without granulocyte colony-stimulating
factor support within 2 weeks prior registration)
2. White Blood Cell (WBC) counts > 2.5 g/L and < 15 g/L
3. Lymphocyte count = 0.5 g/L
4. Platelet count = 100 g/L (without transfusion within 1 week prior registration)
5. Hemoglobin = 90 g/L (Patients may be transfused to meet this criterion but it
should not be done within 1 week prior registration)
6. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and
alkaline phosphatase = 2.5 x ULN (with the exception for patients with documented
liver metastases: AST, ALT and alkaline phosphatase = 5 x ULN)
7. Serum bilirubin = 1.5 x ULN (with the exception for: patients with known or
suspicion of Gilbert disease who have serum bilirubin level = 3 x ULN may be
enrolled)
8. Coagulation: International Normalized Ratio (INR) or Prothrombin Time (PT) = 1.5
X ULN unless subject is receiving anticoagulant therapy as long as PT or Partial
Thromboplastin Time (PTT) is within therapeutic range of intended use of
anticoagulants; Activated Partial Thromboplastin Time (aPTT) =1.5 X ULN unless
subject is receiving anticoagulant therapy as long as PT or PTT is within
therapeutic range of intended use of anticoagulants.
9. Serum albumin > 25 g/L
10. Serum creatinine =1.5 x ULN or creatinine clearance = 40 ml/min on the basis of
Cockcroft-Gault Formula
7. No prior radiation therapy in areas of desired radiation
8. Patients may have had prior therapy provided a washout period of 4 weeks prior to
registration is allowed. Exception: hormone therapy for breast and prostate cancer is
allowed to be continued during the study.
9. Recovery from any toxic effects of prior therapy before registration to = Grade 1 per
the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI
CTCAE v4.03) except for toxicities described below, as they do not put at risk the
patient's condition and do not require systemic immunosuppressive steroids at any
dose, including but not limited to: fatigue, alopecia, skin disorders, stable
neuropathy, endocrinopathies requiring replacement treament.
Note: for other medical conditions, or for any other toxicity with a higher grade but
controlled by adequate treatment, prior discussion and agreement with the trial chair
is mandatory.
Note: Patients may have undergone surgical procedures within the past 3 weeks, as long
as all toxicities have recovered to grade 1 or less.
10. For women of childbearing potential (WOCBP: sexually mature women who have not
undergone a hysterectomy, have not been naturally post-menopausal for at least 12
consecutive months or have a serum follicle-stimulating hormone (FSH) < 40 Millions
International Units (mIU)/ml):
1. Agreement to use 2 acceptable methods of contraception during participation in
the trial and for 6 months after last study combined treatment and after 5 months
of maintenance treatment
2. Women must have a negative urine pregnancy test within 7 days before
registration. A positive urine test must be confirmed by a serum pregnancy test,
or menopausal as per National Comprehensive Cancer Network (NCCN) guidelines.
11. For men: agreement to use 2 acceptable methods of effective contraception during
participation in the trial and for 7 months after last study treatment (combined or
maintenance treatment). Female partners of men who take part in this trial must also
use at least one method of effective contraception during the trial and 7 months after
last study treatment (combined or maintenance).
Exclusion Criteria:
1. Patients with soft tissue sarcoma, glioblastoma, or lymphoma are excluded.
2. Pregnant or breast-feeding women.
3. Symptomatic brain or leptomeningeal disease; any brain metastases must be stable for
at least 6 months
4. Uncontrolled pleural effusion, pericardial effusion, or ascites requiring recurrent
drainage procedures (once monthly or more frequently)
5. Uncontrolled hypercalcemia (> 1.5 mmol/L ionized calcium or Ca > 12 mg/dL or corrected
serum calcium > ULN) or symptomatic hypercalcemia requiring continued use of
bisphosphonate therapy or denosumab
a) Patients who are receiving bisphosphonate therapy or denosumab specifically to
prevent skeletal events and who do not have a history of clinically significant
hypercalcemia are eligible.
6. Life expectancy of < 12 weeks
7. Current, recent (within 4 weeks prior to registration), or planned participation in an
experimental drug study
8. New York Heart Association Class II or greater congestive heart failure
9. History of myocardial infarction or unstable angina within 6 months prior registration
10. History of stroke or transient ischemic attack within 6 months prior registration
11. Significant vascular disease (e.g., aortic aneurysm requiring surgical repair or
recent peripheral arterial thrombosis) within 6 months prior registration)
12. Evidence of bleeding diathesis or significant coagulopathy (in the absence of
therapeutic anticoagulation)
13. Patients with active peptic or duodenal ulceration (within 4 weeks prior to
registration)
14. Current or recent (within 14 days prior registration) treatment use of dipyramidole,
ticlopidine, clopidogrel, cilostazol, prasugrel, or ticagrelor
15. Current or recent (within 14 days prior registration) use of full-dose oral or
parenteral anticoagulants or thrombolytic agents for therapeutic (as opposed to
prophylactic) purpose
1. Prophylactic anticoagulation for the patency of venous access devices is allowed,
provided the activity of the agent results in an International Normalized Ratio
(INR) < 1.5 x ULN and aPTT is within normal limits within 2 weeks prior
registration.
2. Prophylactic use of low molecular-weight heparin (e.g., enoxaparin) is allowed.
16. History of severe allergic, anaphylactic, or other hypersensitivity reactions to
chimeric or humanized antibodies or fusion proteins or allergy to biopharmaceuticals
produced in Chinese hamster ovary cells
17. Known hypersensitivity to any component of the Investigational Medicinal Products
(IMPs)
18. History of autoimmune disease, including but not limited to myasthenia gravis,
myositis, autoimmune hepatitis, systemic lupus erythematosus, rheumatoid arthritis,
inflammatory bowel disease, vascular thrombosis associated with antiphospholipid
syndrome, Wegener's granulomatosis, Sjögren's syndrome, Guillain-Barré syndrome,
multiple sclerosis, vasculitis, or glomerulonephritis. The following exceptions are
considered:
1. Patients with a history of autoimmune-related hypothyroidism on a stable dose of
thyroid replacement hormone are eligible for this study.
2. Patients with controlled Type I diabetes mellitus on a stable insulin regimen are
eligible
19. History of idiopathic pulmonary fibrosis, organizing pneumonia (e.g., bronchiolitis
obliterans), druginduced pneumonitis, idiopathic pneumonitis, or evidence of active
pneumonitis on screening chest Computerized Tomography (CT) scan. Exception: history
of radiation pneumonitis in previous radiation field (fibrosis) is permitted provided
that this area does not undergo current low dose irradiation as part of this protocol.
20. Severe infections within 4 weeks prior registration including but not limited to
hospitalization for complications of infection, bacteremia, or severe pneumonia
21. Signs or symptoms of infection within 2 weeks prior registration
22. Received therapeutic oral or IV antibiotics within 2 weeks prior registration.
Exception: patients receiving routine antibiotic prophylaxis (e.g., to prevent chronic
obstructive pulmonary disease exacerbation or for dental extraction) are eligible.
23. Prior allogeneic stem cell or solid organ transplant
24. Administration of a live, attenuated vaccine within 4 weeks before registration.
Exception: influenza vaccination should be given during influenza season only
(approximately October to March). Patients must not receive live, attenuated influenza
vaccine within 4 weeks prior registration or at any time during the study.
25. Any other diseases, metabolic dysfunction, physical examination finding, or clinical
laboratory finding giving reasonable suspicion of a disease or condition that
contraindicates the use of an investigational drug or that may affect the
interpretation of the results or render the patient at high risk from treatment
complications
26. Patients who have received prior treatment with anti-Programmed Cell Death-1 (PD1),
anti-Programmed Cell Death Ligand-1 (PD-L1) or anti-Cytotoxic T-lymphocyte Associated
4 (CTLA-4) may be enrolled, provided at least 5 half-lives (approximately 75 days)
have elapsed from the last dose to the registration and there was no history of severe
immune-mediated adverse effects from such therapy (NCI CTCAE Grade 3 and 4).
27. Treatment with systemic immunostimulatory agents (including but not limited to
interferon-alpha (IFN-a), interleukin-2 (IL-2)) for any reason within 6 weeks or five
half-lives of the drug, whichever is shorter, prior registration
28. Treatment with systemic immunosuppressive medications (including but not limited to
prednisone, dexamethasone, cyclophosphamide, azathioprine, methotrexate, thalidomide,
and anti-tumor necrosis factor (anti-TNF) agents) within 2 weeks prior registration
1. Patients who have received acute, low-dose, systemic immunosuppressant
medications (e.g., a one-time dose of dexamethasone for nausea) or physiologic
replacement doses (i.e., prednisone 5-7.5 mg/day) for adrenal insufficiency may
be enrolled in the study.
2. The use of inhaled corticosteroids and mineralocorticoids (e.g., fludrocortisone)
is allowed.
|
