Cytokine Expression in Psoriasis Patients With and Without Joint Involvement

Psoriasis Clinical Trial

— CYNEPSA  

Official title:

Cytokine Expression in Sera, ex Vivo Stimulated Peripheral Blood Mononuclear Cells Culture, and Skin in Psoriasis - Comparison of Patients With and Without Joint Involvement

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT05307809
• Other study ID #: CYNEPSA
• Status: Recruiting
• Phase: N/A
• Start date: August 17, 2022
• Est. completion date: August 18, 2024

Study information

• Verified date: January 2024
• Source: Poitiers University Hospital
• Contact: Guillaume LARID
• Phone: +33549444465
• Email: rhumatologie[@]chu-poitiers.fr
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The objective of the resarch is to study the cytokine profile of patients with psoriatic arthritis and psoriasis in order to better understand the pathophysiology of the disease and the contribution of cytokines to the presence or absence of joint involvement. This research will compare cytokines levels in sera, in PBMC culture after LPS or CD3/CD28 agonist antibodies, and in skin biopsy.

Description:

Psoriatic arthritis and Cutaneous Psoriasis are polygenic inflammatory disorders with high burden on the quality of life of patients. Pathophysiology of those disease remain incompletely understood. Recent publications seem to demonstrate an implication of IL-1 family cytokines in the pathogenesis of both diseases. In the literature, results are discordant and focus mainly on cytokines levels in serum of patients. Moreover, few studies compare psoriatic arthritis patients with cutaneous psoriasis patients. We propose here to study cytokines profiles of patients with those conditions in sera, after ex-vivo stimulation of peripheral blood mononuclear cells, and in skin biopsies.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 40
• Est. completion date: August 18, 2024
• Est. primary completion date: August 17, 2024
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 100 Years

Eligibility

Inclusion criteria:

• For psoriasis patients :

• Age over 18 years old

• Signed informed consent

• 5 year old psoriasis at least

• At least one skin lesion over 2 cm

• Without any articular symptoms

• Without any DMARDs

• Without anticoagulant treatments or coagulation disease

• With normal articular and enthesis sonography

• For psoriatic arthritis patients :

• Age over 18 years old

• Signed informed consent

• Psoriatic arthritis according to CASPAR criteria

• Without any DMARDs

• Without anticoagulant treatments or coagulation disease

• With at least one synovitis at inclusion

• For controls

• Age and sex matched with a patient without any rheumatic or dermatologic medical conditions

• No treatment with corticosteroïds or non-steroïdial anti-inflammatory drugsAge over 18 years old

Exclusion criteria:

? For psoriasis patients :

• Joint damage according to CASPAR criteria

• Echography activity in favor of chronic inflammatory rheumatism

• For patients and controls:

• Without social protection rights

• People under judiciary protection, of less than 18 years old, pregnant woman, breast feeding women, jailed patients.

Related Conditions & MeSH terms

• Arthritis, Psoriatic
• Psoriasis
• Psoriatic Arthritis

Intervention

Other:
• Blood sample collection
Blood sample collection
• Skin sample collection
Skin sample collection

Locations

Country	Name	City	State
France	Chu Poitiers	Poitiers

Sponsors (1)

Lead Sponsor	Collaborator
Poitiers University Hospital

Country where clinical trial is conducted

France, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Comparison of cytokine levels in sera, after culture of PBMC stimulated with LPS or CD3/CD28 agonist antibodies between the three groups	Comparison of cytokine levels in sera, after culture of PBMC stimulated with LPS or CD3/CD28 agonist antibodies between the three groups	At inclusion
Secondary	Comparison of cytokine levels in skin biopsies between Psoriatic Arthritis and Cutaneous Psoriasis patients	Comparison of cytokine levels in skin biopsies between Psoriatic Arthritis and Cutaneous Psoriasis patients	At inclusion
Secondary	Correlation of cytokine levels with rheumatologic disease activity, or dermatologic disease activity	Correlation of cytokine levels with rheumatologic disease activity, or dermatologic disease activity	At inclusion