Phenylalanine Requirements in Healthy Pregnant Women

Pregnancy Clinical Trial

Official title:

Dietary Phenylalanine Requirements During Different Stages of Gestation in Healthy Pregnant Women

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02669381
• Other study ID #: H15-02558
• Status: Completed
• Phase: N/A
• Start date: January 1, 2016
• Est. completion date: October 30, 2019

Study information

• Verified date: November 2019
• Source: University of British Columbia
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Phenylalanine is an essential amino acid that people need to obtain in their diets. Amino acids are the building blocks for protein, which is required to form tissues in the body. It is well known that pregnant women require more protein in their diets, but the exact amount for each amino acid is undetermined. To find out how much of phenylalanine pregnant women require, the investigators plan to study pregnant women in early and late gestation with a modern minimally invasive technique.

Description:

The investigator's purpose is to determine the phenylalanine requirements in healthy pregnant women as part of a Master's Thesis project. The determined requirements can be taken into account when giving dietary recommendations, to ensure healthy pregnancies. The hypothesis is that the current recommendations for phenylalanine intake given to pregnant women are underestimated. The investigators also hypothesize that the phenylalanine requirements at early stages of pregnancy will be lower than the requirements towards the later stages of pregnancy.

Current dietary intake recommendations for phenylalanine are based on factorial (mathematical) calculations and do not account for requirement differences between early and late pregnancy. Static recommendations throughout all stages of pregnancy are not appropriate because of the multitude of adaptations the human body goes through during gestation. Whether phenylalanine requirements differ between different stages of gestation is unknown. In addition, phenylalanine is the precursor for tyrosine, which is a conditionally indispensable amino acid. Together they are referred to as the aromatic amino acids. The DRI gives a recommendation for total aromatic amino acids (TAA, phenylalanine + tyrosine) of 36 mg/kg/d during pregnancy. Since this study is the first interested in phenylalanine requirements during pregnancy, the investigators will determine the phenylalanine requirements in the presence of excess tyrosine (61 mg/kg/d) during pregnancy, similar to earlier studies.

The investigators will recruit healthy pregnant women aged 20 to 40 in their 13th-19th week of gestation or 33rd to 39th week of gestation. Once they get in contact with the lab, an initial visit (pre-study) will take place. Here, the participants will be measured for their eligibility and taken through the consent form. They have to participate in a pre-study for each stage of pregnancy, if they wish to participate in both stages. If they meet all the criteria and agree to participate, they will return for up to two study days per stage of 8 hours each. Therefore, if they are recruited during late pregnancy they are only able to participate in two study days in total. During these study days they will be fed protein shakes containing the stable isotope, and then oxidation of this isotope will be measured to determine a breakpoint (requirement) of phenylalanine. Breath samples, urine samples, and a small blood sample will be taken during the study day.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 23
• Est. completion date: October 30, 2019
• Est. primary completion date: May 30, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Female
• Age group: 20 Years to 40 Years

Eligibility

Inclusion Criteria:

• over the age of twenty

• under the age of forty

• in overall good health

• pregnant with a single child

Exclusion Criteria:

• Be under the age of twenty

• Be over the age of forty

• Be pregnant with more than one child

• Have a history of cardiovascular disease, endocrine disorders, or metabolic disorders

• Have recently lost a substantial amount of weight

• Delivered a baby in the last 18 months

• Be substance dependent

• Be allergic to eggs

• Have severe nausea through the pregnancy

• Have gestational diabetes

• Have a history of spontaneous abortions

• Have had an unhealthy pre-pregnancy body mass index

Related Conditions & MeSH terms

• Pregnancy

Intervention

Dietary Supplement:
• Phenylalanine intake
Oral consumption of eight hourly experimental meals- Includes 4 tracer-free experimental meals containing a mixture of free amino acids, calories from a flavored liquid and protein free cookies and 4- labeled amino acid experimental meals.

Locations

Country	Name	City	State
Canada	BC Children's Hospital Research Institute, University of British Columbia	Vancouver	British Columbia

Sponsors (2)

Lead Sponsor	Collaborator
University of British Columbia	Canadian Institutes of Health Research (CIHR)

Country where clinical trial is conducted

Canada, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	13C Phenylalanine Oxidation	Urine, breath and a single blood sample will be collected during the study to measure the rate of oxidation of 13C phenylalanine.	8 hours