Hormonal Changes in Early Pregnancy

Pregnancy Clinical Trial

Official title:

Hormonal Changes in Early Pregnancy: Pilot Study to Evaluate Stored Urine Specimens

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT00340028
• Other study ID #: 999905087
• Secondary ID: 05-E-N087
• Status: Completed
• Start date: January 18, 2005

Study information

• Verified date: September 21, 2023
• Source: National Institutes of Health Clinical Center (CC)
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

This study will analyze hormones in early pregnancy. Hormonal changes in early pregnancy reflect the quality of implantation in the uterus of the fertilized egg, the function of the corpus luteum (mass of hormone-secreting tissue that forms immediately after ovulation) and placenta, and the health of the embryo. It will determine the following: - Whether early pregnancy hormones vary by characteristics of the mother - Whether early pregnancy hormones differ for pregnancies with male or female babies - Whether early pregnancy hormones can predict the gestational age (age of the baby during pregnancy) and the baby's birth weight - Hormone activity at the time pregnancy symptoms begin - How the contribution of the corpus luteum changes during the pregnancy in different women Stored urine specimens collected from women who participated in the North Carolina Early Pregnancy Study in the 1980s will be used for this study. In addition, to evaluate the quality of the stored samples, new specimens will be collected from women currently enrolled in the University of North Carolina's Right from the Start study. Candidates must be white women not of Latino or Hispanic origin who are between 25 and 35 years of age and who are planning to become pregnant within a few months' time. They must be non-smokers, have regular menstrual cycles, no known fertility problems and no major chronic disease. Urine specimens are analyzed for hormone levels and other changes in early pregnancy that can be measured in urine. Women providing new urine specimens do the following: Pre-Pregnancy The pre-pregnancy part of the study lasts through no more than three consecutive menstrual cycles. Participants who do not become pregnant after three menstrual cycles end the study at that time. - Have a short interview with a study staff member - Complete a daily diary, recording any vaginal bleeding or spotting, health status, and use of medications, and mail the diary to the study office once a week - Use an ovulation testing kit for up to 7 days during their menstrual cycle - Call the study staff within the first 24 hours of becoming pregnant Pregnancy The pregnancy part of the study lasts about 5 weeks. - Continue to complete daily diary records for weekly pickup by staff member - Collect urine samples daily and store them in the freezer for weekly pickup by staff member - Have 1 to 2 tablespoons of blood drawn at home weekly for 4 weeks - Have a brief follow-up telephone interview around week 12 of their pregnancy Urine samples are analyzed for hormones such as human chorionic gonadotropin, estrone-3-glucuronide, and pregnanedrol glucuronide. White blood cells are stored for DNA to study such things as genes controlling hormone metabolism.

Description:

Daily first morning urine specimens were collected throughout the first 8-9 weeks of 151 clinical pregnancies. These specimens are now approximately 20 years in storage, and the stability of markers of placental and corpus luteum function are not known. We will collect new urine specimens for 20 pregnancies to serve as a reference to evaluate the quality of those stored samples.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 40
• Accepts healthy volunteers: No
• Gender: Female
• Age group: 25 Years to 35 Years

Eligibility

• INCLUSION/EXCLUSION CRITERIA:

We will recruit from the pre-pregnancy participants in the Right From the Start UNC study entitled, Consequences and Course of Uterine Fibroid in Pregnancies. The women will be selected to be similar to the majority of women in the NC-EPS because we need to have a comparable sample of women in order to best compare hormone levels. Entry requirements will be: white (including Hispanic and latino), age 25-35, regular menstrual cycles, nonsmoker (i.e., no smoking in past year), no major chronic disease, no known fertility problems, no more than 3 months of trying for this current pregnancy.

Related Conditions & MeSH terms

• Pregnancy

Locations

Country	Name	City	State
United States	University of North Carolina	Chapel Hill	North Carolina
United States	NIEHS, Research Triangle Park	Research Triangle Park	North Carolina

Sponsors (1)

Lead Sponsor	Collaborator
National Institute of Environmental Health Sciences (NIEHS)

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	We will assay both old urines and new urines for the estrogen metabolite, the progesterone metabolite, and hCG.	no disease treatment monitored	Time points were daily for urine and weekly blood