Periodontitis Clinical Trial
Official title:
PPAR-γ, RXR, and VDR Expressions in Gingival Tissue Samples of Healthy Individuals, Periodontitis and Peri-implantitis Patients
Verified date | July 2019 |
Source | Tokat Gaziosmanpasa University |
Contact | n/a |
Is FDA regulated | No |
Health authority | |
Study type | Observational |
ABSTRACT Objective: Periodontitis and peri-implantitis are the irreversible destructive
diseases of the periodontal and peri-implant tissues. The present study aimed to determine
the receptor expressions of Peroxisome proliferative-activated receptor (PPAR)-γ, Retinoid X
receptor (RXR)-α, and Vitamin D receptor (VDR) in the diseased tissues around teeth and
dental implants.
Methods: The study consisted of three groups as group 1; healthy controls (C, n=15), group 2;
periodontitis patients with stage 3 grade B (P, n=15), and group 3; peri-implantitis patients
(PI, n=15). Periodontal clinical parameters as the plaque index (PI), gingival index (GI) and
clinical attachment levels (CAL) were recorded. Gingival biopsies were obtained from all
participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin
(H&E) and immunohistochemistry staining were performed. Total inflammatory cell counts and
fibroblast cell density were evaluated on H&E-stained slides while PPAR-γ, RXR-α, and VDR
were evaluated via immunohistochemistry.
Status | Completed |
Enrollment | 45 |
Est. completion date | March 10, 2019 |
Est. primary completion date | January 10, 2019 |
Accepts healthy volunteers | Accepts Healthy Volunteers |
Gender | All |
Age group | N/A and older |
Eligibility |
Inclusion Criteria: - healthy individuals - periodontitis patients - periimplantitis patients Exclusion Criteria: - smokers, - systemically compromised patients |
Country | Name | City | State |
---|---|---|---|
Turkey | Gaziosmanpasa University | Tokat |
Lead Sponsor | Collaborator |
---|---|
Tokat Gaziosmanpasa University |
Turkey,
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Primary | Inflammatory cells counts | Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H&E) staining were performed. Total inflammatory cell counts density were evaluated on H&E-stained slides.A cell counting frame of 10.000 µm2 area was selected under 1000x magnification. The total inflammatory cells (neutrophil, lymphocyte, eosinophil, and macrophage cells) within the frame were counted as inflammatory cell counting. | 6 months | |
Primary | Fibroblast counts | Fibroblasts counts were evaluated from H&E stained slides under 1000x magnification via a light microscope (Nikon Eclipse, E 600, Tokyo, Japan). | 6 months | |
Primary | Peroxisome proliferative-activated receptor (PPAR)-? | Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processin and immunohistochemistry staining were performed, PPAR-?, was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan). | During histological processing | |
Primary | Retinoid X receptor (RXR)-a | Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, RXR-a was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan). | 6 months | |
Primary | Vitamin D receptor (VDR) | . Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, VDR was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan). | 6 months |
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