Lifestyle Modification for MAFLD Based on TTM

Metabolic Associated Fatty Liver Disease Clinical Trial

Official title:

Lifestyle Modification for the Treatment of Metabolic Associated Fatty Liver Disease Based on Transtheoretical Model: a Single-center, Randomized, Controlled Study

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT04871880
• Other study ID #: NFEC-2019-254
• Status: Recruiting
• Phase: N/A
• Start date: May 17, 2020
• Est. completion date: December 30, 2024

Study information

• Verified date: April 2021
• Source: Nanfang Hospital of Southern Medical University
• Contact: Jinjun Chen
• Phone: 8618588531001
• Email: chjj[@]smu.edu.cn
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Lifestyle changes aiming at weight loss remain the cornerstone of MAFLD treatment.Evaluating the motivational stage of patients' change and providing targeted lifestyle guidance may significantly improve the efficiency of weight loss. The investigators hypothesize that gut microbiota may affect motivation to lose weight, and the changes in gut microbiota due to weight loss may positively feedback the behavior of motivation, forming a virtuous circle. Thus, this study aims at ①evaluating the relationships between motivational stage of weight loss and the gut microbiota (Gut-brain axis); ②investigating the effects of lifestyle interventions on the gut microbiota in MAFLD patients.

Description:

Fatty liver associated with metabolic dysfunction is common, affects a quarter of the population, and has no approved drug therapy. In 2020, two position articles have proposed not only the change of the terminology from NAFLD to metabolic associated fatty liver disease (MAFLD), but also an update and revision of the definition of fatty liver disease. According to these papers, indeed, the diagnosis of MAFLD can be made with the presence of hepatic steatosis (detected by serum biomarker scores, imaging methods or histology) and at least one of the fol- lowing metabolic criteria: (a) overweight/obesity, (b) T2DM and (c) metabolic dysregulation (that means at least two factors among increased waist circumference, hypertension, hypertriglyceridemia, low serum HDL-cholesterol levels, impaired fasting glucose, homeostatic model assessment of insulin resistance [HOMA-IR] > 2.5 or high sensitivity C-reactive protein > 2 mg/L). Here，the investigators focus on the obese NAFLD, which considered as a sub-phenotype in MAFLD.

Lifestyle changes aiming at weight loss remain the cornerstone of MAFLD treatment. However, it is not easy to make patients change their unhealthy lifestyle. Behavior therapy may only be successful in motivated patients, and motivation to dieting and exercising may be different according to age and sex. Personal motivation for change plays a pivotal role in behavior changes. The transtheoretical model (TTM) is an empirically validated model of individual behavioral change, which involves progress through a series of stages to make a particular behavioral change. TTM-based interventions have been applied to facilitate health behavioral changes, such as physical exercise, smoking cessation, and weight management, studies of which continue to demonstrate positive effects. Evaluating the motivational stage of patients' change and providing targeted lifestyle guidance may significantly improve the efficiency of weight loss. The investigators hypothesize that gut microbiota may affect motivation to lose weight, and the changes in gut microbiota due to weight loss may positively feedback the behavior of motivation, forming a virtuous circle.

Thus, a total of seventy obese patients with NAFLD will be recruited in this study, divided into lifestyle modification program group(n=35) and usual care group(n=35). All subjects will undergo dietary assessment based on Food Frequency Questionnaire ( FFQ) and motivational stages evaluation based on TTM. Stool samples will be collected at each visit. Demographic data will be recorded, consisted of age, weight, height, waist circumference, BMI, and so on. MRI-PDFF will be performed to evaluate liver steatosis. Meanwhile, vibration-controlled transient elastography will be carried out, which include liver stiffness (LSM) and controlled attenuation parameter (CAP) measurements. Biochemistry tests will be conducted as supplementary for assessment of NAFLD and cardiovascular risks, comprising liver function test, lipid, fasting glucose, etc.

The investigators aim at ①evaluating the relationships between motivational stage of weight loss and the gut microbiota (Gut-brain axis); ②investigating the effects of lifestyle interventions on the gut microbiota in MAFLD patients.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 75
• Est. completion date: December 30, 2024
• Est. primary completion date: May 17, 2024
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 80 Years

Eligibility

Inclusion Criteria:

1. With age range of 18-65 years.

2. BMI = 28kg/m2

3. Fatty infiltration of the liver was confirmed on imaging studies (ultrasound, transient elastography, computed tomography, or magnetic resonance imaging) or liver biopsy.

4. With valid motivational stage assessment based on TTM.

5. Written consent form obtained.

Exclusion Criteria:

1. History of other chronic liver disease (Viral hepatitis B or C, autoimmune hepatitis, cholestatic and Inherited metabolic liver diseases) and hemochromatosis.

2. Self-reported HIV-positive status, active tuberculosis, active malaria, or inflammatory bowel disease.

3. Excessive alcohol consumption (>30g/d for men and >20g/d for women).

4. Patient has known cirrhosis (compensated/decompensated) either based on clinical criteria or liver histology or Imaging techniques.

5. Subjects using thyroid hormones, oestrogens, amiodarone, steroids, tamoxifen and other medicine known to affect liver fat accumulation.

6. Subjects using thiazolidinedione hypoglycemic drugs, vitamin E and other medicine have potential benefits for NASH within six months.

7. Subjects with organ failure.

8. Subjects with hepatocellular carcinoma or other active malignancy.

9. Solid organ transplant recipients.

10. Antibiotic treatment within the previous 3 months.

11. Suffering from any acute or chronic cardiovascular, GI or immunological condition.

12. Gastric or duodenal ulcer in the past six months.

13. Currently pregnant or nursing.

14. Concomitant diseases with reduced life expectancy.

15. Combined mental illness.

16. Contraindication to MRI scanning.

17. Exclusions may also be made at the discretion of the attending physician or the eligibility committee.

18. Inability to provide informed consent.

Related Conditions & MeSH terms

• Fatty Liver
• Liver Diseases
• Metabolic Associated Fatty Liver Disease

Intervention

Behavioral:
• Dietitian led life style modification intervention
All participants received recommendations for a low-fat hypocaloric diet that was 750 kcal/day less than their daily energy need which supervised by dietitians. Additionally, patients were encouraged to walk 200 minutes per weeks and a short self-report questionnaire for the measurement of habitual physical activity was implemented at baseline, 4, 16, 32 and 48 weeks.
• Conventional care (control)
Receive routine care

Locations

Country	Name	City	State
China	Hepatology Unit, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University	Guangzhou	Guangdong

Sponsors (1)

Lead Sponsor	Collaborator
Nanfang Hospital of Southern Medical University

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Hepatic fat	Change of liver proton density fat fraction (PDFF) in MAFLD assessed by magnetic resonance imaging (MRI)	12 months
Primary	Obesity	Changes in body weight	1, 4, 8, 12, 24 months
Primary	Gut microbiota	Changes in bacterial component of the gut microbiome	1, 4, 8, 12, 24 months
Secondary	Blood pressure	Changes in blood pressure	1, 4, 8, 12, 24 months
Secondary	Central obesity	Changes in waist circumference	1, 4, 8, 12, 24 months
Secondary	Insulin resistance	Changes in insulin resistance eveluated by HOMA-IR	1, 4, 8, 12, 24 months
Secondary	FPG	Changes in fast plasma glucose	1, 4, 8, 12, 24 months
Secondary	HbA1c	Changes in HbA1c	1, 4, 8, 12, 24 months
Secondary	Plasma triglycerides	Changes in plasma triglycerides	1, 4, 8, 12, 24 months
Secondary	Plasma HDL-cholesterol	Changes in plasma HDL-cholesterol	1, 4, 8, 12, 24 months
Secondary	Plasma high-sensitivity C-reactive protein level	Changes in plasma high-sensitivity C-reactive protein level	1, 4, 8, 12, 24 months
Secondary	Gut microbiota diversity	Changes in diversity of gut microbiome(a-diversity and ß-diversity)	1, 4, 8, 12, 24 months