Polymorphism, Restriction Fragment Length Clinical Trial
Official title:
The Effect of an Acute Maximal Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism
Verified date | September 2020 |
Source | Eastern Mediterranean University |
Contact | n/a |
Is FDA regulated | No |
Health authority | |
Study type | Interventional |
This study aims to determine the effect of an acute maximal exercise on serum uncoupling
protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations.
This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this
eventual effect.
Therefore, this study hypothesizes that:
H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6
levels are different before and after an acute maximal exercise.
H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated
with uncoupling protein 1-3826 A/G polymorphism.
H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal
exercise on serum uncoupling protein 1 levels.
H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated
with uncoupling protein 1-3826 A/G polymorphism.
H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of
maximal exercise on serum lipid and lipoprotein levels.
Status | Completed |
Enrollment | 86 |
Est. completion date | September 7, 2020 |
Est. primary completion date | September 2, 2020 |
Accepts healthy volunteers | Accepts Healthy Volunteers |
Gender | Male |
Age group | 18 Years to 35 Years |
Eligibility |
Inclusion Criteria: - Athletes group: - Must be basketball, or volleyball, or handball players. - Must be subjects who have been training regularly for at least 3 months. - Sedentary group: * Must be subjects who have not been training regularly for at least 3 months. - For both groups: - Healthy. - Non obese (body mass index < 30). - Aged between 18 and 35 years. Exclusion Criteria: - For both groups: - Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances. - Being obese (body mass index > 30). - Having an acute or chronic injury which is limiting physical performance. - Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples. - Being injured or sick during the study. |
Country | Name | City | State |
---|---|---|---|
Turkey | Ege University Faculty of Sport Sciences | Izmir |
Lead Sponsor | Collaborator |
---|---|
Eastern Mediterranean University | Ege University |
Turkey,
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Other | Change from the baseline serum C reactive protein level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. C reactive protein analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum creatine kinase level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatine kinase analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum alanine aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Alanine aminotransferase analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum aspartate aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Aspartate aminotransferase analyzes were performed from serum samples in the Medical Biochemistry Laboratory of the Ege University Faculty of Medicine. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline hemogram test at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Hemogram test analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline at serum glucose level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Glucose analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum urea level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Urea analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum creatinine level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatinine analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum total cholesterol level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total cholesterol analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum low density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Low density lipoprotein analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum high density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. High density lipoprotein analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum triglyceride level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Triglyceride analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum total antioxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total antioxidant analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline serum total oxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total oxidant analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Other | Change from the baseline heparin plasma thiobarbituric acid reactive substances level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Thiobarbituric acid reactive substances analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Primary | Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Primary | Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Primary | Uncoupling protein-1-3826 A/G polymorphism determination | Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory. | 15 minutes before the Yo-Yo intermittent recovery test (level 1) | |
Secondary | Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Interleukine-6 analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | |
Secondary | Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Nitric oxide analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) |
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