Role of Ascorbic Acid Infusion in Critically Ill Patients With Transfusion Related Acute Lung Injury

Acute Lung Injury, Transfusion Related Clinical Trial

— ASTRALI  

Official title:

Role of Ascorbic Acid Infusion in Critically Ill Patients With Transfusion Related Acute Lung Injury

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04153487
• Other study ID #: ASTRALI
• Status: Completed
• Phase: Phase 2
• Start date: November 30, 2019
• Est. completion date: July 16, 2021

Study information

• Verified date: May 2021
• Source: Damanhour University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

TRALI was defined as "acute noncardiogenic pulmonary edema typically occurs ≤ 6 hours following transfusion of plasma-containing blood products, such as packed red blood cells, fresh frozen plasma, platelets, or cryoprecipitate." In critically ill patients, TRALI remains the leading cause of transfusion-related fatalities and is accompanied by a very significant morbidity and mortality. Survival in such patients is as low as 53% compared with 83% in acute lung injury (ALI) controls.

The incidence of TRALi is likely underreported. In densely populated developing countries, incidence has not decreased due to lack of male-only strategy for plasma donation.

TRALI is associated with systemic inflammation characterized by low anti-inflammatory cytokine as interleukin (IL)-10, increased pro-inflammatory cytokine as IL-8. Regulation of inflammation should include avoidance of overproduction of inflammatory mediators. So, it can be dampened not only by increasing IL-10 but also by decreasing IL-1β release. C-reactive protein (CRP) is an acute phase protein which is up-regulated during infections and inflammation. CRP was recently identified as a novel first hit in TRALI.

Till now, there is no established treatment for TRALI beyond supportive care and monitoring. Recently, potential therapies have been reviewed, and it was concluded that the most promising therapeutic strategies are IL-10 therapy, downregulation of CRP levels, targeting reactive oxygen species (ROS) or blocking IL-8 receptors. So, antioxidants (such as high dose vitamins), were recommended for future studies as potentially effective treatment.

Vitamin C hypovitaminosis is observed in 70% of critically ill despite receiving recommended daily doses.

The aim of this study is to investigate the role of intravenous vitamin C (ascorbic acid) as a targeted therapy for transfusion related acute lung injury (TRALI) in critically ill patients in terms of IL-8, IL-10, CRP, SOD, malondialdehyde (MDA), vasopressor use, duration of mechanical ventilation, ICU length of stay, 7-days mortality and 28-days mortality.

Description:

1. Ethical committee approval will be obtained from Ethics committee of Faculty of Pharmacy, Damanhour University.

2. The minimum required sample size is estimated to be 40 patients for each group.

3. Full written informed consent will be taken from all patients or their next of kin to participate in this study.

4. All patients will be subjected directly at time of enrollment to the following;

• Complete history taking and demographic data

• The potential recipient risk factors for TRALI.

• The initial cause of ICU admission and the blood products received.

• Complete physical examination including chest auscultations.

• Vital signs

• Routine laboratory investigations

• Brain natriuretic peptide level

• Troponin T

• Hypoxic index

• Acute Physiology and Chronic Health Evaluation version II (APACHE II) score.

• Sequential Organ Failure Assessment (SOFA) score.

• Kidney Disease Improving Global Outcomes (KDIGO) criteria.

• Child Pugh score.

• Chest radiography and transthoracic echocardiography.

5. Samples will be drawn to measure the initial values of ascorbate level, plasma IL-8, IL-10, IL-1β, SOD, MDA and serum CRP.

6. Eighty patients with confirmed TRALI (n=80) will be enrolled from critical care units (tertiary hospitals). Then, in addition to their supportive and standard care, they will be randomized (computer sheet) into two groups:

• ASTRALI (AScorbic acid in TRALI) group (n=40) will receive 2.5 gm vitamin C intravenously every 6 hrs for 96 hrs from diagnosis.

• Control group (n=40) will receive placebo in similar regimen.

7. All patients will be followed up and treated during the study time. All relevant routine investigations, supportive measures, medications and ventilatory data will be recorded.

8. All possible adverse events will be monitored, recorded and managed directly. Hyperoxaluria, microscopic calcium-oxalate crystallization or oxalate nephropathy will be monitored, recorded and managed directly.

9. After 96 hrs, resampling for ascorbate level and the same biomarkers will be done.

10. Measuring the study secondary outcomes will include vasopressor use, duration of mechanical ventilation, ICU length of stay, 7-days mortality and 28-days mortality.

11. Statistical tests appropriate to the study design will be conducted to evaluate the significance of the results.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 40
• Est. completion date: July 16, 2021
• Est. primary completion date: July 16, 2021
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 64 Years

Eligibility

Inclusion Criteria:

• Adult (18 - 64 years) critically ill patients diagnosed with transfusion related acute lung injury (TRALI), at the time of enrollment or maximum 6 hours before, according to the National Heart, Lung and Blood Institute (NHLBI) Working Group definitions and or the Canadian Consensus Conference criteria (29, 30) as the following criteria;

• No evidence of ALI prior to transfusion.

• Onset of ALI = 6 hours following cessation of transfusion.

• Hypoxemia, defined as the ratio of arterial oxygen partial pressure to fractional inspired oxygen (PaO2/FiO2) = 300 mmHg or oxygen saturation = 90% on room air.

• Radiographic evidence of bilateral infiltrates.

• No evidence of left atrial hypertension.

Exclusion Criteria:

• Pregnancy or breastfeeding.

• Hypernatremia or known hypersensitivity to the study drug.

• Parenteral nutrition (total/partial) containing vitamin C.

• Active renal stone or history of urolithiasis.

• Acute Kidney Injury.

• Glucose 6 phosphate dehydrogenase deficiency, iron and copper storage diseases.

• Immunocompromised patients (cancer or patients on immunosuppressive drugs).

• Moribund patient not expected to survive 24 hours .

• Home mechanical ventilation (via tracheotomy or noninvasive) except for Continuous Positive Airway Pressure/ Bilevel Positive Airway Pressure (CPAP/BIPAP) used only for sleep-disordered breathing .

Related Conditions & MeSH terms

• Acute Lung Injury
• Acute Lung Injury, Transfusion Related
• Lung Injury
• Transfusion-Related Acute Lung Injury
• Wounds and Injuries

Intervention

Drug:
• Ascorbic Acid Injectable Product
Intermittent Intravenous Infusion of Ascorbic Acid (Vitamin C) 2.5 gm / 6 hours for 96 hours
• Placebo
Placebo saline / 6 hours for 96 hours

Locations

Country	Name	City	State
Egypt	Damanhour University	Beheira

Sponsors (2)

Lead Sponsor	Collaborator
Damanhour University	Alexandria University

Country where clinical trial is conducted

Egypt, 

References & Publications (36)

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* Note: There are 36 references in all — Click here to view all references

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Interleukin-8 (IL-8)	Plasma level of IL-8	96 hrs
Primary	Interleukin-10 (IL-10)	Plasma Level of IL-10	96 hrs
Primary	C-reactive protein (CRP)	Serum level of CRP	96 hrs
Primary	Superoxide Dismutase (SOD)	Plasma Level of SOD	96 hrs
Primary	Malondialdehyde (MDA)	Plasma level of MDA	96 hrs
Secondary	Vasopressor use (days)	Duration of circulatory support	up to 28 days
Secondary	Duration of Mechanical Ventilation (days)	Duration of ventilatory support	up to 28 days
Secondary	ICU length of stay (days)	Length of stay in ICU	Up to 28 days
Secondary	7-days Mortality	All cause mortality	7 days
Secondary	28-days Mortality	All cause mortality	28 days