Polymorphism, Restriction Fragment Length Clinical Trial
Official title:
Relationship Between the VEGF Gene Polymorphism and Cardiometabolic Risk Factors in People With Excessive Body Weight in the Polish Population
The aim of the study is to determine the relationship between VEGF gene expression, VEGF gene polymorphism and serum leptin concentration in the Polish population in people with excessive body weight. In addition, the aim of the study is to look for relationships between the VEGF gene polymorphism and anthropometric and biochemical factors of cardiovascular risk and endothelial dysfunction such as body weight, waist circumference, serum total cholesterol, LDL, HDL, triglycerides, glucose and the occurrence of cardiovascular diseases in the family of a patient with excessive body weight in the Polish population.
400 people (250 - study group; 150 control group) were subjected to subjective and objective
action. Information on the occurrence of diseases and cardiovascular risk in the participant
and his family was collected from research studies. Anthropometric parameters were measured
(body weight, height, BMI, waist circumference, neck circumference) as well as blood pressure
and pulse measurement. In addition, fasting venous blood was collected and secured. In venous
blood currently marked with the following concentration: glucose, required cholesterol, LDL
cholesterol, HDL cholesterol and triglycerides. In addition, a method of salting out and
protecting DNA was developed from blood.
Furthermore the polymorphisms of VEGF genes (in positions: -2578 and -634) using the HMR
(High Resolution Melt) method was determined.
Also VEGF and leptin by ELISA was determined. After obtaining the results of comparative
analysis of the correlation between the occurrence of VEGF gene polymorphisms, serum levels
of leptin and VEGF, and anthropometric and biochemical parameters of cardiovascular risk.
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