Uterine Peristaltic Frequencies Between Pregnant and Non-pregnant Women in Embryo Transfer Cycles Clinical Trial
Official title:
Comparison of Uterine Peristaltic Wave Frequencies Between Pregnant and Non-pregnant Women in Embryo Transfer Cycles
Eligibility criteria
- Infertile woemn undergo IVF & embryo transfer cycles at infertility clinic at King
Chulalongkorn Memorial Hospital Measurement
- Uterine peristaltic wave frequencies & Junctional zone thickness by 3D-TVUS on the day
of oocyte retrieval in fresh embryo transfer participants & day before progesterone
supplementation in frozen-thawed embryo transfer participants
- Serum progesterone & estradiol level in the same day
The patients who were planning to undergo fresh embryo transfer cycles used GnRH antogonist
protocol. Ovarian stimulation was initiated on the menstrual cycle day 3 with use of daily
subcutaneous injection of gonodotrophin; human menopausal gonadotrophin (Menopur®),
recombinant FSH-alpha (Gonal-F®), recombinant FSH-beta (Puregon®). After 5 days of
stimulation, the dosage of gonadotrophin was adjusted based on ovarian response, as assessed
by follicular size monitoring and serum estradiol levels. To prevent premature LH surges,
ganirelix (Orgalutran®) was administered by subcutaneous injection when at least one follicle
reached a diameter of 13 mm. When the leading follicle reached 18 mm in diameter, final
oocyte maturation was triggered by subcutaneous injection of recombinant hCG (Ovidrel®) 0.25
mg or double triggered by addition subcutaneous injection of triptorelin (Diphereline®) 0.1
mg. Oocyte retrieval was performed 36 hours after drug administration. Insemination of
retrieved oocytes was performed by intracytoplasmic sperm injection (ICSI). Progesterone
supplement was started on the day of ovum pickup with 400 mg-micronized vaginal progesterone
pessary (Cyclogest®) two times a day. Embryo transfer was performed on day 4 to day 6 of
progesterone supplement.
The patients who were planning to undergo frozen embryo transfer cycles used artificial
endometrial preparation or natural cycles. Endometrial priming was achieved with daily oral
estradiol valerate (Progynova®) 6-8 mg per day beginning on the
cycle day 3 for 12 days. Endometrial thickness was assessed by transvaginal ultrasonography.
Once the endometrium thickness reaches 8 mm with trilaminar appearance, 400-mg micronized
vaginal progesterone pessary was started daily. Embryo transfer was performed on day 4 to day
6 of progesterone supplement. In the participants were not achieved the goal, dosage of oral
estradiol valerate was increased and/or 50 microgram-transdermal estradiol hemihydrates
(Climara®) was added. Two to three days after adjusted dose, the endometrial thickness was
reassessed and progesterone was administered as mentioned above if the goal was reached.
Cycle cancellation was chosen if the thickness was not optimal.
Embryo transfer was performed following ASRM guidance using either soft or metallic catheter.
The procedure was done gently avoiding fundal contact with transabdominal ultrasonography
monitoring.
All participants were examined uterine peristaltic wave frequencies and thickness of
junctional zone two times by three- dimensional transvaginal ultrasonography (3D-TVUS). The
first measurement was performed on day 2 of menstrual cycle. The second measurement was
performed on the day planning to start progesterone administration in frozen-thawed embryo
transfer protocol and the day of oocyte retrieval in fresh embryo transfer protocol.
Three-dimensional ultrasonography machine, Voluson S6 (GE, USA), was used with RIC5-9W
real-time 4D micro-convex endovaginal curved linear probe by single operator. All
participants were instructed to avoid sexual activity before evaluation for two days. The
patient was lying relaxed in a lithotomy position after emptying the bladder. To avoid
stimulating the cervix, the probe was gently introduced into the vagina and uterine
peristaltic wave was measured first. Two-minute video of fixed mid-sagittal plane of uterus
was recorded. Three dimensional sonographic features were obtained by applying volume box
covered the mid-sagittal plane of uterus. Coronal plane of uterus was appeared after
automatic sweep of mechanical transducer by multiplanar and OMNI view. Speckle reduction
imaging (SRI) and volume contrast imaging (VCI) modality was set up at SRI 0-2 and VCI 2-4
mm. Thickness of junctional zone was measured as the distance from the basal endometrium to
the internal layer of the outer myometrium, which appeared as relative hypoechoic linear
lining around the endometrium. All sides of uterine wall were evaluated; fundal, lateral,
anterior, and posterior wall. Each side was measured at two regions, the thickest and the
thinnest distance and the average thickness of each wall was obtained by dividing of the sum
of thickest and thinnest area. Finally, the average thickness of four uterine walls was
obtained. Uterine peristaltic wave frequencies were analyzed with 4X of regular speed using a
VLC media player by two independent observers. The mean of
peristaltic wave frequencies between two persons were obtained if the frequencies were not
concordance.
Hormonal levels were checked for analysis two times in each patient. First measurement, serum
luteinizing hormone (LH) and estradiol hormone levels were measured at day 2 of menstrual
cycle in all participants. The patients in fresh embryo transfer cycles, serum follicle
stimulating hormone (FSH) measurement was added. Second measurement, serum estradiol and
progesterone hormone levels were checked in the same day of 3D-TVUS assessment.
Two weeks after embryo transfer, serum beta-hCG was first measured. In the patients with
rising hCG level, serum level was reassessed at week 3 and week 4 after embryo transfer and
transvaginal ultrasonography was performed at week 4 after the transfer. Vaginal progesterone
was continued in the same dose and intramuscular 17-hydroxyprogesterone caproate (Depot-
proluton®) was added weekly. Luteal support was continued until 12 weeks of gestation. In
non-pregnant women, vaginal progesterone was stopped after first measurement of serum
beta-hCG. Clinical pregnancy was defined as the presence of intrauterine gestational sac four
weeks after embryo transfer.
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