Complication Following Ectopic Pregnancy Clinical Trial
Official title:
Vascular Endothelial Growth Factor Levels in Ectopic Pregnancy
The aim of this study is to determine plasma Vascular Endothelial Growth Factor level and
change in patient with ectopic pregnancy who needs to surgical treatment.
Patient with ectopic pregnancy diagnosed and treated with medically or surgically will be
included in the study. This is an observational study.The plasma Vascular Endothelial Growth
Factor level will be measured first day and two day after the diagnosis of ectopic
pregnancy.
This study is a prospective, observational, single-center study. Ethical approval was
obtained from the Institutional Review Board. This study will be completed with 30 patients
with ectopic pregnancy. An intervenous cannula will be centrifuged for 15 minutes at 1000 g
within 30 minutes of collection. Aliquots of plasma will be added to eppendorf tubes and
stored at -20°C.
Plasma Vascular Endothelial Growth Factor levels will be measured using a Human VEGF-A
Platinum ELISA kit (bioscience company, Catalog No: 00122, Santa Clara, California, USA).
All assays will be performed according to the manufacturer's instructions. The test kit
sensitivity is 2.5 pg/ml. Intracycle variability is %4-6, intercycle variability is %8-10.
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Observational Model: Case-Only, Time Perspective: Prospective