Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In- Vitro Study

Osteosarcoma Clinical Trial

— ESWT  

Official title:

Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In- Vitro Study

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05801887
• Other study ID #: 02_D012_112292
• Status: Completed
• Phase: N/A
• Start date: October 22, 2021
• Est. completion date: November 6, 2022

Study information

• Verified date: March 2023
• Source: Krishnadevaraya College of Dental Sciences & Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. It has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis. The present study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability. It will be assessed using a scratch assay, Mtt assay and ATP analysis.

Description:

Introduction: Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis. Aim: This study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability. Methodology: In this in-vitro study, 20 Osteoblast - like cells will be included. 10 samples to be considered for Control group and the remaining 10 samples considered as Test group. Extracorporeal shockwave will be applied to the Test samples. They will be further analysed using wound scratch assay which is used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay used to determine Cell viability after exposure to shockwaves. The cell migration and cell viability after exposure to shockwaves will determine its effects on osteoblast-like cells.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 20
• Est. completion date: November 6, 2022
• Est. primary completion date: August 17, 2022
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: N/A and older

Eligibility

Inclusion Criteria:

• Cell cultures with a confluency of 70%-80%

Exclusion Criteria:

• Contaminated cell cultures

Related Conditions & MeSH terms

• Osteosarcoma

Intervention

Device:
• Extracorporeal shockwave device
Extracorporeal shockwave device generates true shock waves which are characterized by a sudden rise in pressure followed by an exponential decay. This is different from the commercially available shockwave devices in which they use piezoelectric based mechanisms which generate high frequency waves.
• No ESWT
the osteoblast like cells are not exposed to shockwaves

Locations

Country	Name	City	State
India	Dr. Roxanne	Bengaluru	Karnataka

Sponsors (1)

Lead Sponsor	Collaborator
Krishnadevaraya College of Dental Sciences & Hospital

Country where clinical trial is conducted

India, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Scratch assay	cell migration	72hours
Primary	MTT Assay and ATP analysis	Cell viability	74hrs