Microbiota Transplant to Cancer Patients Who Have Failed Immunotherapy Using Faeces From Clinical Responders

Non-small Cell Lung Cancer Clinical Trial

— MITRIC  

Official title:

MITRIC: Microbiota Transplant to Cancer Patients Who Have Failed Immunotherapy Using Faeces From Clincal Responders

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT05286294
• Other study ID #: MITRIC
• Status: Recruiting
• Phase: Phase 2
• Start date: June 28, 2022
• Est. completion date: December 31, 2034

Study information

• Verified date: April 2024
• Source: Oslo University Hospital
• Contact: Jon Amund Kyte, MD, Ph.D.
• Phone: +4722934000
• Email: jonky[@]ous-hf.no
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This is a single-arm, single-center, open-label, phase IIa study evaluating the safety, feasibility and efficacy of Faecal Microbiota Transplant (FMT) to cancer patients not responding to ICI therapy, using ICI-responders as donors.

Description:

Immunotherapy with immune checkpoint inhibitors (ICI) has shown remarkable clinical efficacy against several cancer forms. This includes durable responses in patients with metastatic cancers and no other effective treatment options. However, many patients do not respond. This leaves a major challenge; how to turn non-responders into responders. Herein, this challenge is addressed, by attempting to modulate patients' intestinal microbiota through Faecal Microbiota Transplant (FMT). Data from several preclinical and translational studies have indicated that the microbiota composition is important for the effect of ICIs. Moreover, two recent trials exploring FMT for melanoma patients have suggested acceptable safety and a potential clinical benefit.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 20
• Est. completion date: December 31, 2034
• Est. primary completion date: March 31, 2034
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

1. Participant must be 18 years of age, at the time of signing the informed consent

2. Histologically confirmed malignant melanoma, NSCLC, CSCC, HNSCC, renal clear cell carcinoma or MSI+ solid cancer

3. Metastatic disease or local recurrence not curable by standard therapy

4. PD-L1 positivity is required for subjects with HNSCC (>20% combined positive score) and NSCLC (>20% PD-L1 expression)

5. Measurable disease according to iRECIST

6. Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1

7. Progressive disease, as considered by the treating physician, on therapy with PD1/PD-L1 blockers and/or CTLA4-blockers and/or LAG-3 blockers, or combinations regimes comprising any of these agents. Further treatment with ICI is considered to be within standard practice.

8. Patients without any response to ICI at any time point during their disease history are eligible, without a need for re-introduction of ICI before enrollment, even if subsequent lines of anti-cancer therapy have been given. For patients with prior

response to ICI, the criteria depend on the cancer form:

1. Malignant melanoma, NSCLC and MSI-H/dMMR solid cancers: Prior response to ICI is allowed only if PD under ICI has been documented <9 months before enrolment and without subsequent lines of anti-cancer therapy. For patients with prior response to ICI followed by subsequent lines of anti-cancer therapy, and patients that have not received ICI the last 9 months, ICI has to be re-introduced, and these patients have to again show progressive disease while on ICI therapy.

2. CSCC, HNSCC and renal clear cell carcinoma: Prior response to ICI is allowed, without a need for re-introduction, even if subsequent lines of anti-cancer therapy have been given, provided that disease progression has been documented under ICI therapy the last 12 months.

9. Mandatory pre-FMT biopsy and lesion accessible for further biopsies

10. Life expectancy >3 months

11. Adequate organ function as defined below:

1. Hemoglobin > 9 g/dL

2. Absolute neutrophil count (ANC) = 1.0 x 109/L

3. Platelet count =80 x 109/L

4. INR=1.2

5. Serum bilirubin =1.5 x institutional upper limit of normal (ULN).

6. AST and ALT =2.5 x ULN unless liver metastases are present, in which case it must be =5x ULN

7. Albumin >25 g/L

8. Serum creatinine =1.5 X ULN OR measured creatinine clearance (CL) >40 mL/min or Calculated creatinine CL>40 mL/min by the Cockcroft-Gault formula (Cockcroft and Gault 1976) or by 24-hour urine collection for determination of creatinine clearance.

12. Capable of giving signed informed consent

Exclusion Criteria:

1. Other cancer within 3 years prior to study entry, with the exception of those with a negligible risk of metastasis or death and treated with expected curative outcome (such as adequately treated carcinoma in situ of the cervix or basal or squamous cell skin cancer)

2. Spinal cord compression not definitively treated with surgery and/or radiation, or previously diagnosed and treated spinal cord compression without evidence that disease has been clinically stable for > 2 weeks prior to first FMT

3. Uncontrolled pleural effusion, pericardial effusion, or ascites. Patients with indwelling catheters are allowed

4. Uncontrolled tumor-related pain. Patients requiring narcotic pain medication must be on a stable regimen at study entry. Symptomatic lesions (e.g., bone metastases or metastases causing nerve impingement) amenable to palliative radiotherapy should be treated prior to enrolment. Asymptomatic metastatic lesions whose further growth would likely cause functional deficits or intractable pain (e.g., epidural metastasis that is not presently associated with spinal cord compression) should be considered for loco-regional therapy if appropriate prior to enrolment.

5. Significant cardiovascular disease, such as New York Heart Association (NYHA) cardiac disease (Class II or greater), myocardial infarction within 3 months prior to study entry, unstable arrhythmias, or unstable angina. Patients with a known left ventricular ejection fraction (LVEF) < 40% will be excluded. Patients with known coronary artery disease, congestive heart failure not meeting the above criteria, or LVEF < 50% must be on a stable medical regimen that is optimized in the opinion of the treating physician, in consultation with a cardiologist if appropriate

6. Undergone allogeneic stem cell or solid organ transplantation

7. A positive test for HIV

8. Active hepatitis B (defined as having a positive hepatitis B surface antigen [HBsAg] test at screening) or hepatitis C. Patients with past hepatitis B virus (HBV) infection or resolved HBV infection (defined as having a negative HbsAg test and a positive antibody to hepatitis B core antigen [anti-HBc] antibody test) are eligible. Patients positive for hepatitis C virus (HCV) antibody are eligible only if polymerase chain reaction (PCR) is negative for HCV RNA.

9. Active tuberculosis

10. Ongoing immune-related adverse effects from immunotherapy treatments that are of Grade =2, excluding endocrine adverse effects. An ongoing grade 2 cutaneous reaction is allowed.

11. Severe infection within 14 days prior to first FMT, requiring hospitalization.

12. Any condition that significantly increases the risk of perforation during endoscopy for FMT.

13. A history or current evidence of any condition, therapy, or laboratory abnormality that might confound the results of the trial, interfere with the subject's participation for the full duration of the trial, or is not in the best interest of the subject to participate, in the opinion of the treating Investigator.

14. Known psychiatric or substance abuse disorders that would interfere with cooperation and the requirements of the trial

15. A requirement of systemic antibiotics at the time of study entry.

16. Received oral or IV antibiotics within 5 days prior to first FMT.

17. Currently receiving other study therapy that may interfere with the interpretation of data in this study.

18. Received treatment with systemic corticosteroids or other systemic immunosuppressive medications (including but not limited to prednisone, dexamethasone, cyclophosphamide, azathioprine, methotrexate, thalidomide, and anti-tumor necrosis factor [TNF] agents) within 10 days prior to first FMT, or anticipated requirement for systemic immunosuppressive medications during the trial. A daily dose equivalent to =10mg prednisolone is allowed.

1. Patients who have received acute, low-dose, systemic immunosuppressant medications (e.g., a one-time dose of dexamethasone for nausea) may be enrolled in the study.

2. Patients with a history of allergic reaction to IV contrast requiring steroid pre-treatment should have baseline and subsequent tumor assessments performed using MRI.

3. The use of inhaled corticosteroids for chronic obstructive pulmonary disease, mineralocorticoids (e.g., fludrocortisone) for patients with orthostatic hypotension, and low-dose supplemental corticosteroids for adrenocortical insufficiency are allowed.

19. Pregnant or breastfeeding

20. Any reason why, in the opinion of the investigator, the patient should not participate

Related Conditions & MeSH terms

• Carcinoma
• Carcinoma, Renal Cell
• Carcinoma, Squamous Cell
• Clear Cell Renal Cell Carcinoma
• Cutaneous Squamous Cell Carcinoma
• Head and Neck Squamous Cell Carcinoma
• Melanoma Stage IV
• Non-small Cell Lung Cancer
• Squamous Cell Carcinoma of Head and Neck

Intervention

Biological:
• Fecal Microbiota Transplant (FMT)
Fecal Microbiota Transplant (FMT)

Locations

Country	Name	City	State
Norway	Oslo University Hospital	Oslo

Sponsors (1)

Lead Sponsor	Collaborator
Oslo University Hospital

Country where clinical trial is conducted

Norway, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Immunological response evaluation	Immunological changes in blood (PBMC, serum/plasma), gut and tumor tissue	10 years
Other	Explorative assessment of biomarkers or clinical responses and toxicity	Microbial gut composition, tumor tissue, blood and gut will be characterized and correlated with treatment outcome. Assessment will include baseline profile and changes after FMT	10 years
Other	Investigation of T cell reactivity to neoantigens and microbial antigens	T cell receptor binding and T cell reactivity to selected peptides	10 years
Other	Characterization of tumor evolution and changes in immunological milieu induced by the FMT and continued ICI therapy	Comparison of tumor biopsies at baseline and later time points, by gene and protein profiling	10 years
Primary	Safety evaluation of Fecal Microbiota Transplant (FMT) in advanced cancer patients	Safety of the intervention (FMT) as assessed by the incidence, nature, and severity of Adverse Events (AE) according to NCI CTCAE, version 5.0	10 years
Primary	Tumor response evaluation	Objective Tumor Response Rate (ORR) as assessed by iRECIST in FAS	10 years
Secondary	Feasibility evaluation of FMT for advanced cancer patients	The percentage of included patients that receive i) at least two FMT, ii) the scheduled number of FMT until End of Intervention (EOI)	10 years
Secondary	Overall survival (OS)	Clinical response estimation	10 years
Secondary	Objective Tumor Response Rate (ORR)	Clinical response estimation	10 years
Secondary	Progression Free Survival (PFS)	Clinical response estimation	10 years
Secondary	Durable response rate (DRR)	Clinical response estimation	10 years
Secondary	Clinical benefit range (CBR)	Clinical response estimation	10 years
Secondary	Duration of objective response (DOR)	Clinical response estimation	10 years
Secondary	Implant engraftment estimation, and the effect of repeated FMT	Comparison of the patients' gut bacterial composition before and after FMT and in relation to their donors	10 years
Secondary	Evaluation of the effect of therapy on quality of life	Patient reported outcomes (PRO) as assessed by EORTC quality of life questionnaire (QLQ-C30)	10 years
Secondary	Evaluation of the effect of therapy on fatigue	Patient reported outcomes (PRO) as assessed by Chalder Fatigue Questionnaire (FQ)	10 years
Secondary	Evaluation of the effect of therapy on pain	Patient reported outcomes (PRO) as assessed by an 11-point Numerical Rating Scale (NRS) for pain intensity	10 years