Time Course of Postprandial Protein Metabolism

Muscle Protein Synthesis Clinical Trial

Official title:

Time-dependent Regulation of Postprandial Muscle Protein Synthesis After Milk Protein Ingestion in Young Men

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04121689
• Other study ID #: 14234
• Status: Completed
• Phase: N/A
• Start date: October 1, 2014
• Est. completion date: August 26, 2019

Study information

• Verified date: August 2019
• Source: University of Illinois at Urbana-Champaign
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The anabolic action of 'fast' whey protein on the regulation of postprandial muscle protein synthesis has been established to be short-lived in healthy young adults. Our aim was assess the time course of anabolic signaling events and stimulation of muscle protein synthesis rates (MPS) after ingestion of a food source that represents a more typical meal-induced pattern of aminoacidemia, namely milk protein concentrate, in healthy young males.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 7
• Est. completion date: August 26, 2019
• Est. primary completion date: November 1, 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 35 Years

Eligibility

Inclusion criteria

• Males

• Aged between 18-35 years

• Healthy, recreationally active

• BMI < 30 kg/m2

Exclusion criteria

• Smoking

• Allergies to milk proteins

• Vegetarians

• Diagnosed GI tract diseases

• Female

• Arthritic conditions

• A history of neuromuscular problems

• Previous participation in amino acid tracer studies

• Predisposition to hypertrophic scarring or keloid formation

• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).

Related Conditions & MeSH terms

• Muscle Protein Synthesis
• Protein Metabolism

Intervention

Dietary Supplement:
• Milk Protein Concentrate
The macronutrient composition and energy of the milk protein beverage provided to participants is 38 g protein (3.46 g leucine), 4.17 g carbohydrate, and 1.4 g fat. The milk protein met all chemical and bacteriologic specifications for human consumption. The L-[1-13C]phenylalanine and L-[1-13C]leucine enrichments in the milk protein concentrate averaged 38.3 and 10.8 mole percent excess (MPE), respectively.

Locations

Country	Name	City	State
United States	Freer Hall	Urbana	Illinois

Sponsors (1)

Lead Sponsor	Collaborator
University of Illinois at Urbana-Champaign

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Fractional synthesis rates of myofibrillar protein	Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion. This will allow us assess the timecourse of muscle protein synthesis after eating.	8 hours
Secondary	Phosphorylation of muscle anabolic signaling	Phosphorylation of anabolic signaling pathways (mTORC1, AKT, and P70S6K) involved in the stimulation of myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hours, and every 1-2 hours during the 5 hours after protein ingestion.	8 hours
Secondary	Exogenous rate of phenylalanine appearance	Dietary derived phenylalanine availability will be measured during the 5 h postprandial phase to determine its relationship with the fractional synthesis rates of myofibrillar protein and phosphorylation of muscle anabolic signaling	5 hours