One Week of Magnesium Supplementation Lowers IL-6, Perceived Pain and Increases Post Exercise Blood Glucose in Response to Downhill Running

Magnesium Deficiency Clinical Trial

Official title:

One Week of Magnesium Supplementation Lowers IL-6, Perceived Pain and Increases Post Exercise Blood Glucose in Response to Downhill Running

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04216836
• Other study ID #: SH17180029-R
• Status: Completed
• Phase: N/A
• Start date: June 28, 2018
• Est. completion date: March 1, 2019

Study information

• Verified date: January 2020
• Source: University of Worcester
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study investigated the effect of magnesium supplementation on exercise performance and functional recovery in recreational endurance athletes in conjunction with measures of blood glucose, lactate, IL-6 and sIL-6R.

Description:

Magnesium status can directly affect circulating glucose concentrations both during and post exercise. In addition, magnesium supplementation has been shown to reduce circulating IL-6 concentrations post exercise in humans. It is conceivable that such observations are linked through the role of IL-6 in glucose regulation, possibly in combination with sIL-6R. Together, magnesium intake may have the potential to effect exercise performance and recovery through glucose availability. This in turn may be connected to the production of IL-6 and sIL-6R which have been established to influence exercise fatigue and perception of pain (muscle soreness).

Recruitment information / eligibility

• Status: Completed
• Enrollment: 9
• Est. completion date: March 1, 2019
• Est. primary completion date: March 1, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 20 Years to 35 Years

Eligibility

Inclusion Criteria:

• Regular recreational runner, running around 3 times per week

• Capable of running 10 km in ~ 40 minutes.

Exclusion Criteria:

• Any signs or symptoms of cardiovascular issues.

• Any recent form of injury or illness.

• Currently, or in the last 3 months, have consumed multivitamin supplements

• Currently, or in the last 3 months, have consumed anti-inflammatory medications.

Related Conditions & MeSH terms

• Magnesium Deficiency

Intervention

Dietary Supplement:
• Magnesium oxide
Magnesium capsule
• Placebo
Cornflour capsule manufactured to mimic the 166.6 mg magnesium capsule.

Locations

Country	Name	City	State
United Kingdom	University of Worcester	Worcester	Worcestershire

Sponsors (3)

Lead Sponsor	Collaborator
University of Worcester	Beijing Sport Uninversity, Coventry University

Country where clinical trial is conducted

United Kingdom, 

References & Publications (9)

Chen HY, Cheng FC, Pan HC, Hsu JC, Wang MF. Magnesium enhances exercise performance via increasing glucose availability in the blood, muscle, and brain during exercise. PLoS One. 2014 Jan 20;9(1):e85486. doi: 10.1371/journal.pone.0085486. eCollection 2014. — View Citation

Chen YJ, Chen HY, Wang MF, Hsu MH, Liang WM, Cheng FC. Effects of magnesium on exercise performance and plasma glucose and lactate concentrations in rats using a novel blood-sampling technique. Appl Physiol Nutr Metab. 2009 Dec;34(6):1040-7. doi: 10.1139/H09-105. — View Citation

Cheng SM, Yang LL, Chen SH, Hsu MH, Chen IJ, Cheng FC. Magnesium sulfate enhances exercise performance and manipulates dynamic changes in peripheral glucose utilization. Eur J Appl Physiol. 2010 Jan;108(2):363-9. doi: 10.1007/s00421-009-1235-y. Epub 2009 Oct 9. — View Citation

Dmitrašinovic G, Pešic V, Stanic D, Plecaš-Solarovic B, Dajak M, Ignjatovic S. ACTH, Cortisol and IL-6 Levels in Athletes following Magnesium Supplementation. J Med Biochem. 2016 Nov 2;35(4):375-384. doi: 10.1515/jomb-2016-0021. eCollection 2016 Oct. — View Citation

Febbraio MA, Steensberg A, Keller C, Starkie RL, Nielsen HB, Krustrup P, Ott P, Secher NH, Pedersen BK. Glucose ingestion attenuates interleukin-6 release from contracting skeletal muscle in humans. J Physiol. 2003 Jun 1;549(Pt 2):607-12. Epub 2003 Apr 17. — View Citation

Glund S, Deshmukh A, Long YC, Moller T, Koistinen HA, Caidahl K, Zierath JR, Krook A. Interleukin-6 directly increases glucose metabolism in resting human skeletal muscle. Diabetes. 2007 Jun;56(6):1630-7. Epub 2007 Mar 15. — View Citation

Gray SR, Ratkevicius A, Wackerhage H, Coats P, Nimmo MA. The effect of interleukin-6 and the interleukin-6 receptor on glucose transport in mouse skeletal muscle. Exp Physiol. 2009 Aug;94(8):899-905. doi: 10.1113/expphysiol.2009.048173. Epub 2009 May 29. — View Citation

Heffernan SM, Horner K, De Vito G, Conway GE. The Role of Mineral and Trace Element Supplementation in Exercise and Athletic Performance: A Systematic Review. Nutrients. 2019 Mar 24;11(3). pii: E696. doi: 10.3390/nu11030696. — View Citation

Robson-Ansley P, Cockburn E, Walshe I, Stevenson E, Nimmo M. The effect of exercise on plasma soluble IL-6 receptor concentration: a dichotomous response. Exerc Immunol Rev. 2010;16:56-76. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Downhill 10 km treadmill time trial performance	Maximal 10 km time trial performance on a treadmill	1 day
Primary	24 hr post exercise maximal force testing of the dominant leg on the isokinetic dynamometer	Maximal force produced from the dominant leg (eccentric and concentric) on an isokinetic dynamometer.	1 day
Secondary	Glucose	Capillary blood samples at rest, during, immediately post, 1 hr post and 24 hrs post 10 km downhill time-trial. A Biosen analyser was used to analyse glucose concentrations.	up to 2 days
Secondary	Interleukin-6	Venous blood samples at rest, immediately post, 1hr post and 24 hrs 10 km downhill time trial. Enzyme-linked immunosorbent assays were used to analyse interleukin-6.	up to 2 days
Secondary	Soluble interleukin-6 receptor	Venous blood samples at rest, immediately post, 1hr post and 24 hrs 10 km downhill time trial. Enzyme-linked immunosorbent assays were used to analyse soluble interleukin-6.	up to 2 days
Secondary	Perceived muscle soreness	A 10cm visual analogue scale was used to assess perceived muscle soreness, the scale started at 0 (no pain) and finished at 10 (unbearable pain)	up to 4 days
Secondary	Lactate	Capillary blood samples at rest, during, immediately post, 1 hr post and 24 hrs post 10 km downhill time-trial. A Biosen analyser was used to analyse lactate concentrations.	up to 2 days
Secondary	Creatine kinase	Venous blood samples at rest, immediately post, 1hr post and 24 hrs 10 km downhill time trial. A Reflotron analyser was used to analyse creatine kinase concentrations.	up to 2 days