Adding Cytokines to In Vitro Human Culture Media to Improve Embryogenesis and Implantation

Infertility Clinical Trial

— GHLCSHED  

Official title:

The Effects of GM-CSF, HB-EGF and LIF Cytokines Supplementation Need Verification for Human Embryo Development in Vitro

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02420886
• Other study ID #: Ibnsina IVF center
• Status: Completed
• Phase: N/A
• Start date: May 16, 2015
• Est. completion date: March 2016

Study information

• Verified date: September 2018
• Source: Ibn Sina Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Assessment of the embryological benefit of in vitro adding of Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2n/ml) to the culture media and closely monitor its effect on embryogenesis and implantation outcomes.

Description:

The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2ng/ml) as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 443
• Est. completion date: March 2016
• Est. primary completion date: March 2016
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Female
• Age group: 18 Years to 40 Years

Eligibility

Inclusion Criteria:

• all patients

Exclusion Criteria:

• over 40 years

Related Conditions & MeSH terms

• Infertility

Intervention

Other:
• Adding LIF, HB-EGF and GM-CSF to Lifeglobal human IVF culture media

Locations

Country	Name	City	State
Egypt	Banon IVF Center	Assiut
Egypt	Ibnsina IVF center	Sohag

Sponsors (2)

Lead Sponsor	Collaborator
Ibn Sina Hospital	Banon IVF Center Assiut, Egypt

Country where clinical trial is conducted

Egypt, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	ongoing pregnancy rate ( % )		6 months
Secondary	clinical pregnancy rate ( % )		6 months
Secondary	implantation rate		6 months
Secondary	fertilization rate		6 days of culture
Secondary	top-quality embryo rate		6 days of culture
Secondary	rates for blastocyst formation and quality		6 days of culture
Secondary	embryo utilization rate		6 days of culture