Clinical Trials Logo

Clinical Trial Summary

In crossover trials, ten (N=10) young men (18-35 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after resistance exercise, participants will ingest stable isotope labeled whole eggs (18 g protein, 17 g fat) or egg whites (18 g protein, 0 g fat) cooked in scrambled form. Repeated blood and muscle biopsies will be collected to determine whole body amino acid kinetics, muscle amino acid transporters, anabolic signaling and myofibrillar protein synthesis rates during the trials.


Clinical Trial Description

On both infusion trials, participants will report to the laboratory at 0700 h after an overnight fast. Upon arrival to the lab, a baseline breath sample will be collected for determination of 13CO2 enrichment by isotope ratio mass spectrometry. A Teflon catheter will be inserted into an antecubital vein for baseline blood sample collection (t=-210 min) and then participants will receive priming doses of NaH13CO2 (2.35 µmol·kg-1), L-[1-13C]leucine (7.6 µmol·kg-1), and L-[ring-2H5]phenylalanine (2.0 µmol·kg-1). Subsequently, a continuous intravenous infusion of L-[1-13C]leucine (0.10 µmol·kg-1·min-1) and L-[ring-2H5]phenylalanine (0.05 µmol·kg-1·min-1) will be initiated (t=-210 min) and maintained over the infusion trials. A second Teflon catheter willinserted into a heated dorsal hand vein for repeated arterialized blood sampling and remained patent by a 0.9% saline drip. Breath samples and arterialized blood samples and will be collected every 30 or 60 min during the postabsorptive- and postprandial states. In the post-absorptive state of infusion trial 1, muscle biopsies will be collected at t=-150 and -30 min of infusion to determine basal-state myofibrillar protein synthesis rates, relative skeletal muscle amino acid transporter content, and anabolic-related signaling. In the subsequent cross-over trial only 1 muscle biopsy will be collected at t=-30 for Western blot analysis and postabsorptive myofibrillar protein-bound tracer enrichment. After collection of the resting muscle biopsy at t=-30 for both trials, the participants will perform resistance exercise that consists of 4 sets of 10 repetitions at 80% of 10-RM for both leg press and leg extension exercise.

Immediately after completion of the exercise bout, participants will consume 3 whole eggs or an equivalent amount of protein from egg whites (t=0 min). Completion of the meal will mark the start of the postprandial phase (t=0 min) and additional muscle biopsies will be collected at t=120 and 300 min. ;


Study Design


Related Conditions & MeSH terms


NCT number NCT03117127
Study type Interventional
Source University of Illinois at Urbana-Champaign
Contact
Status Completed
Phase N/A
Start date July 2015
Completion date May 2017

See also
  Status Clinical Trial Phase
Recruiting NCT05945641 - Effect of Low-load Resistance Training vs. High-intensity Interval Training on Local Muscle Endurance N/A
Not yet recruiting NCT05544955 - Protein Ingestion Timing on Body Composition and Biochemical Markers in Resistance-trained Males N/A
Completed NCT04411173 - Effect of Daily Doses of Rice or Whey Protein on Resistance Training Adaptations N/A
Completed NCT04381390 - Egg Consumption and Muscular Adaptations N/A
Completed NCT04535596 - Blood Flow Restriction Exercises and Conservative Exercises in Knee Osteoarthritis N/A
Completed NCT04213586 - Effects of Whey Protein and Collagen Supplementation N/A
Completed NCT03918395 - Supplemental Protein And Resistance Training Adaptations N/A
Completed NCT05058794 - Comparison Among the Effects of Blood Flow Restriction Training and Conventional Exercise in Knee Osteoarthritis. N/A
Recruiting NCT04750694 - High Intensity Resistance Training Combined With Blood Flow Restricted Exercise in Elite Handball Players N/A
Completed NCT06121869 - Dileucine and Resistance Training Adaptations N/A
Active, not recruiting NCT03572127 - Mycoprotein, Resistance Training, and Hypertrophy N/A
Not yet recruiting NCT06075901 - Reliability and Performance Analysis of the Use of Tissue Flossing for Blood Flow Restriction. N/A
Recruiting NCT05774444 - Investigating the Effects of Krill Oil and Krill Protein on Post-exercise Muscle Protein Metabolism N/A
Recruiting NCT05576337 - Neuromuscular Characteristics of Strict Vegetarians and Non-Vegetarians Women N/A
Withdrawn NCT04256343 - D2O Dosing Strategies to Assess Muscle Protein Synthesis N/A
Completed NCT04845295 - Resistance Training Effects on Muscle Morphological, Mechanical and Contractile Properties N/A
Completed NCT03870165 - Food First Approach to Stimulate Muscle Protein Synthesis in Healthy Adults N/A
Recruiting NCT03565302 - Beta2-adrenergic Agonism and Muscle Remodelling N/A
Not yet recruiting NCT06164249 - Quantification of Internal Training Load N/A
Completed NCT03723226 - BFR and Muscle Mitochondrial Oxidative Capacity N/A