Effects of Anesthetic Methods on Hepatic Cancer Cell Malignancy

Hepatocellular Carcinoma Clinical Trial

Official title:

Effects of Serum From Hepatocellular Carcinoma Surgery Patients Under Different Anesthetic Methods on Hepatic Cancer Cell Malignancy in Vitro

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03594188
• Other study ID #: HEP201807
• Status: Completed
• Phase: N/A
• Start date: July 23, 2018
• Est. completion date: December 31, 2019

Study information

• Verified date: April 2020
• Source: RenJi Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Numerous studies found that anesthetic methods can influence the recurrence of tumor and the overall survival of patients after primary cancer surgery. Radiofrequency (RF) ablation is now widely used in clinic for treatment of hepatocellular carcinoma (HCC). Currently, diverse anesthetic methods, including general anesthesia (GA), epidural anesthesia and local anesthesia (LA), are used for RF ablation surgery. Using serum from HCC surgery patients randomized to receive either GA or LA during surgery, we investigated the effects of anesthetic methods on proliferation, migration and metastasis in HepG2 hepatic cancer cells in vitro.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 24
• Est. completion date: December 31, 2019
• Est. primary completion date: July 31, 2019
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 65 Years

Eligibility

Inclusion Criteria:

• patients with a single primary liver tumor of 3cm or smaller, who are scheduled for RF ablation surgery

Exclusion Criteria:

• less than 18 or more than 65 years old;

• ASA Physical Status 4 or greater;

• previous surgery in liver (including radiofrequency ablation);

• severe systemic disease (heart, lung, kidney, or immune system);

• INR>1.5 or platelet count <45,000 cells/mm3;

• a history of addiction to opioids;

• with known extension beyond the liver;

• Child-Pugh Class C.

Related Conditions & MeSH terms

• Carcinoma
• Carcinoma, Hepatocellular
• Hepatocellular Carcinoma
• Liver Neoplasms

Intervention

Procedure:
• anesthetic methods
Patients will receive standard GA or LA for RF surgery.

Locations

Country	Name	City	State
China	Renji Hospital affliated to Shanghai Jiaotong University School of Medicine	Shanghai	Shanghai

Sponsors (1)

Lead Sponsor	Collaborator
RenJi Hospital

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	cell proliferation	HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA for 24h and cell proliferation will be measured	1 hour post-surgery
Secondary	cell migration	HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell migration will be measured	1 hour post-surgery
Secondary	cell metastasis	HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell metastasis will be measured	1 hour post-surgery