Effect of Amino Acid on Muscle Damage

Healthy Clinical Trial

Official title:

Effect of Leucine-enriched Essential Amino Acid on Integrated Muscle Protein Synthesis and Muscle Recovery After Resistance Exercise

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03319147
• Other study ID #: EAA
• Status: Completed
• Phase: N/A
• Start date: October 18, 2017
• Est. completion date: October 30, 2019

Study information

• Verified date: April 2021
• Source: University of Toronto
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The primary objective is to determine the impact of essential amino acids on integrated muscle protein synthesis over 4 days after resistance exercise.

In addition, the secondary objective is to determine the effect of essential amino acids on satellite cell regulation and inflammatory responses during this prolonged recovery period.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 20
• Est. completion date: October 30, 2019
• Est. primary completion date: January 15, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

• Healthy, male, recreationally-active participants

• Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix. B). Active will be defined as being in the top 20% of age specific scores on iPAQ (the International Physical Activity Questionnaire; Appendix.C).

• Participants are required not to engage in lower limb RT (resistance training) for at least 6 months prior to the study.

• Participants will be 18-35 years old.

• Participants are willing to abide by the compliance rules of this study

Exclusion Criteria:

• Female

• Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor

• Self-reported regular tobacco use

• Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.)

• The individual who has allergy for milk

Related Conditions & MeSH terms

• Healthy

Intervention

Dietary Supplement:
• Active
4g of essential amino acids
• Placebo
4g of maltdextrin

Locations

Country	Name	City	State
Canada	Goldring Centre for High Performance Sport	Toronto	Ontario

Sponsors (2)

Lead Sponsor	Collaborator
University of Toronto	Ajinomoto Co., Inc.

Country where clinical trial is conducted

Canada, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Muscle protein synthesis	Skeletal muscle fractional synthesis rate (%/h) will be determined by oral pulse method using ring-[D5]phenylalanine	4 hours
Secondary	exercise performance	Maximal strength (torque in n-M) of the knee extensors will be assessed by a maximal isometric voluntary contraction (MVC).	4 days
Secondary	Muscle soreness	Corresponding subjective ratings of muscle soreness will be collected from participants using a visual analogue scale (mm)	4 days
Secondary	Muscle damage	Muscle damage will be determined by histochemical analysis. muscle damage is defined as Z-band streaming.	4 days