Healthy Clinical Trial
Official title:
The Influence of High and Low Salt on Exosomes in the Urine
The hypothesis is that changes in the epithelial sodium channel (ENaC) in the kidney is reflected in urinary exosomes and that the amount of ENaC as well as the cleavage degree is upregulated in conditions with low salt.
Methods:
Exosomes will be isolated from spot urine by ultracentrifugation and tested using western
blotting. In-house developed antibodies against different parts of the ENaC gamma subunit as
well as commercial antibodies will be used. A marker for exosomes will validate the presence
of exosomes in the urine.
Power considerations:
In this cross over salt-intervention study with quantification of ENaC the investigators
refer to a published study regarding the sodium-chloride co-transporter (NCC) in the kidney,
were rats where given aldosterone infusion or a low salt diet. In this study n=6 was
sufficient to detect a 2-3 fold change in NCC in urinary exosomes. Furthermore 5 patients
with primary aldosteronism had a significant increase in NCC in urinary exosomes (N. Lubbe et
al, Hypertension 2012).
On the basis of this the investigators choose to test 10 persons, but are not able to make a
regular power calculation.
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