The Impact of Protein Intake on Protein Metabolism During Intensified Training

Healthy Clinical Trial

Official title:

The Impact of Protein Intake on Amino Acid and Protein Metabolism, and Performance During Intensified Training

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02801344
• Other study ID #: PFE
• Status: Completed
• Phase: N/A
• Start date: May 2016
• Est. completion date: February 2018

Study information

• Verified date: March 2019
• Source: University of Toronto
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake.

The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 12
• Est. completion date: February 2018
• Est. primary completion date: September 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 50 Years

Eligibility

Inclusion Criteria:

• Healthy, male, endurance-trained participants who have run regularly more than 45 km or 4.5 hours/week

• Participants who are categorized at least "very good" based on a study by Shvartz & Reibold [40], in which peak rate of oxygen consumption(VO2 Peak) is used as an index. (i.e. the participants whose VO2peak is =57 ml/kg/min (18-24 y), =54 ml/kg/min (25-29 y), =52 ml/kg/min (30-34 y), =49 ml/kg/min (35-39 y), =47 ml/kg/min (40-44 y), =44 ml/kg/min (45-50 y) according to his age,

• Participants who can cover 10 km in less than 60 min after the VO2peak test and 5 km Time trial on session 2.

• Participants will be 18-50 years old.

• Participants are willing to abide by the compliance rules of this study

Exclusion Criteria:

• Inability to meet health and physical activity guidelines according to the The Physical Activity Readiness Questionnaire for everyone.

• Female

• Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor

• Regular tobacco use

• Illicit drug use (e.g. growth hormone, testosterone, etc.)

Related Conditions & MeSH terms

• Healthy

Intervention

Dietary Supplement:
• Normal
test drink which contains low amount of amino acids (0.14 g/kg/day)
• Moderate
test drink which contains moderate amount of amino acids (0.40 g/kg/day)
• High
test drink which contains high amount of amino acids (1.03 g/kg/day)

Other:
• Controlled-diet
the diet containing 0.8 g protein /kg/day

Locations

Country	Name	City	State
Canada	Goldring Centre For High Performance Sport	Toronto	Ontario

Sponsors (2)

Lead Sponsor	Collaborator
University of Toronto	Ajinomoto Co., Inc.

Country where clinical trial is conducted

Canada, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Creatine Phosphate Kinase activity on day 5	Muscle damage markers (blood Creatine Phosphate Kinase activity, IU/L) will be compared between the experimental arms 5days after the beginning of training	5days after the beginning of training
Other	Myoglobin concentration on day 5	Muscle damage markers (blood Mb Concentration, mg/mL) will be compared between the experimental arms 5days after the beginning of training	5days after the beginning of training
Other	IL-6 concentration on day 5	inflammatory markers (IL-6 concentration, pg/ml) will be compared between the experimental arms 5days after the beginning of training	5days after the beginning of training
Other	CRP concentration on day 5	inflammatory markers (CRP concentration, mg/L) will be compared between the experimental arms 5days after the beginning of training	5days after the beginning of training
Other	amino acid concentration on day 5	amino acid concentration (mmol/mL) will be compared between the experimental arms 5days after the beginning of training	5days after the beginning of training
Primary	Change in 24h whole body protein balance	Protein balance (mg/kg/d; protein synthesis - protein breakdown) will be measured using [15N]Glycine method on day 1 and day 4 to determine the change over the intensified training period to compare between protein levels	Difference between day 1 and day 4 whole body protein balance
Secondary	Change from baseline physical performance (5 km time trial) 5days after the beginning of training	Before and after 4 days of intensified training period, Duration (seconds) for completing 5-km running on treadmill will be measured.	5days after the beginning of training
Secondary	change from baseline Physical performance (muscle strength) 5days after the beginning of training	Maximal strength will be assessed by a maximal isometric voluntary contraction (MVC), which will be tested for the knee-extensors using a custom-made knee-extension apparatus after 4 days intensified training.	5days after the beginning of training
Secondary	change from baseline Physical Performance (muscle power) 5days after the beginning of training	Peak lower body power will be measured by force platform after 4days-intensified training.	5days after the beginning of training