Clinical Trial Details
— Status: Recruiting
Administrative data
| NCT number |
NCT02582853 |
| Other study ID # |
GBS CD163 |
| Secondary ID |
1-10-72-256-151- |
| Status |
Recruiting |
| Phase |
N/A
|
| First received |
October 19, 2015 |
| Last updated |
October 21, 2015 |
| Start date |
September 2015 |
| Est. completion date |
January 2018 |
Study information
| Verified date |
October 2015 |
| Source |
University of Aarhus |
| Contact |
Malalai Musleh, Student |
| Phone |
+45 27134658 |
| Email |
malalaimusleh[@]hotmail.com |
| Is FDA regulated |
No |
| Health authority |
Denmark: The National Committee on Health Research EthicsDenmark: Danish Dataprotection Agency |
| Study type |
Observational
|
Clinical Trial Summary
Guillain- Barré syndrome (GBS) is an acute inflammatory demyelinating polyneuropathy (AIDP)
that often is triggered by an infection. GBS is characterized by progressing weakness and
numbness and loss of tendon of reflexes. It can also include tingling sensation in the legs
and arms. These symptoms occur due to an autoimmune attack on the myelin resulting in
demyelination.
The diagnosis is given by electrophysiological examination and clinical presentation.
GBS is treated with intravenous immunoglobulin (IVIG) and plasma exchange (PE). Both
treatments are equally effective. Most patients recover completely, while others must ease
symptoms and reduce the duration of illness by several treatments.
The purpose of this study is to define if patients with GBS have higher concentrations of
sCD163 in their cerebrospinal fluid and serum compared with symptomatic control subjects.
Furthermore it is to define if the concentrations of sCD163 reduces after treatment.
Description:
Guillain- Barré syndrome (GBS) is an acute inflammatory demyelinating polyneuropathy (AIDP)
that often is triggered by an infection. GBS is characterized by progressing weakness and
numbness and loss of tendon reflexes. It can also include tingling sensation in the legs and
arms. These symptoms occur due to an autoimmune attack on the myelin resulting in
demyelination. The diagnosis is given by electrophysiological examination and clinical
presentation. GBS is treated with intravenous immunoglobulin (IVIG) or plasma exchange (PE).
The specific mechanism is not fully understood, but it is believed that the antibodies
produced against the myelin, is eliminated to avoid further damage to the peripheral nerves.
IVIG is slightly safer and easier to give than PE. However, both treatments are equally
effective. They both need to be given during the first few weeks of symptoms.
Most patients recover completely, while others must ease symptoms and reduce the duration of
illness by several treatments. Moreover, the initial phase of GBS is followed by a long
recovery period where intensive neurorehabilitation is important. Some people will not
recover completely from GBS and experience long-term complications such as not being able to
walk unaided, loss of sensation causing lack of coordination and balance. It is estimated
around 20% of people with GBS still experience some muscle weakness after three years. Some
may also have persistent fatigue. In rare cases, complications can be life threatening,
particularly in the acute phase.
GBS is one of the neurological emergencies, where patients need to be examined closely
during the initial acute phase of the illness. Many studies have been performed to elucidate
the mechanism of neuropathy in Guillain Barré syndrome but treatment remains disappointing.
There are findings of autoantibodies against gangliosides, myelin proteins such as PO-
glycoprotein, P2 basic protein and PMP22 and to nodal and paranodal proteins, such as NF155
and NF186.
Cluster of differentiation 163 (CD-163) - a new way to measure GBS CD-163 is a scavenger
receptor (recognizes and uptakes macromolecules), which marks the monocyte-macrophage
activation. CD163 works for macrophages as a receptor for haemoglobin-haptoglobin complexes.
The soluble form of the receptor is called sCD163 and is found in plasma, where it is up
regulated in a large range of inflammatory diseases.
Values, which are increased strongly (<20mg/l), are seen in diseases like macrophage
activating syndrome, haemophagocytic syndrome and acute hepatic failure. Furthermore, the
level of sCD163 reflects the activity of the disease. Patients with increasing and high
values are found to have unfavourable prognosis. Increased values (5-20 mg/l) are seen in
hepatic disease, sepsis and Gaucher´s disease. Septic patients with values greater than
10mg/l have a 10 times greater risk of fatal outcome. Values, which are slightly increased
(3-5 mg/l), are often seen in inflammatory of infectious conditions and in cancer.
Purpose and Hypothesis Purpose To define the concentration of sCD163 in the cerebrospinal
fluid and serum in patients with GBS patients compared with controls.
The project hypothesis Our hypothesis is that the inflammatory biomarker sCD163 is increased
in CSF and blood from GBS patients compared to controls Perspectives Due to the various
clinical variants of inflammatory polyneuropathies and due to the lack of biomarkers, it
remains difficult to diagnose GBS. It is very important to identify new methods for
diagnosis and monitoring of patients with GBS. This may also enable better prediction of the
prognosis and the level of treatment needed.
The pathology of GBS is still unknown but it is known to be immune mediated. In several
studies sCD163 has been shown to up regulated in a larger range of inflammatory diseases
suggesting sCD163 to be a relevant biomarker for GBS.
Study population
The study population will consist of two subgroups:
1. Patients diagnosed with GBS Patients will undergo a lumbar puncture as a part of the
diagnostic procedure as soon as they are suspected to be suffering from GBS on clinical
grounds with blood test. The procedure and a blood test will be repeated after 6
months.
2. Symptomatic controls Controls include patients with unspecified neurological symptoms
or diseases who will undergo a lumbar puncture at the Department of Neurology at Aarhus
University Hospital as part of their diagnostic work up irrespective of this study. The
investigators expect to include 40 symptomatic controls.
Due to admission to the Department of Neurology, Aarhus University Hospital with the
diagnosis of suspected GBS all eligible participants will receive information about the
project and will be asked to give their consent to participation.
As mentioned the controls are recruited patients, who nevertheless will undergo lumbar
puncture for another reason to the Department of Neurology, will be informed about the
project and will be asked to participate. Control patients will be participating only once.
The investigators anticipate including 20 patients with GBS and 40 symptomatic controls.
Participants will receive written and oral information about the study. The oral information
will take place at Department of Neurology, Aarhus University Hospital. Moreover, the
pamphlet " Før du deltager i et sundhedsvidenskabeligt forsøg"(about participating in a
clinical trial) will be handed out.
The information will be given by consultant Henning Andersen, MD Lars Markvardsen or stud.
med. Malalai Musleh. It is possible for the participants to bring a companion and they are
offered about an hour to consider the participation because the procedure of lumbar puncture
will take place at the same day. If the patient need to consider the participation with a
companion and it is not possible for the participants to bring a companion within an hour,
then they will not be included in the study.
The participants will be informed of that information from the medical record will be passed
on and included and used in this project including past medical history, actual use of
drugs, results of biochemical analyses and brain imaging, abnormalities at the clinical
examinations and the conclusions from the doctors diagnostic workup.
If new knowledge about disadvantages of participating in the study appears the participants
will be informed immediately.
Moreover, the participant will be informed if evaluations during the study bring knowledge
about the general health status of the patient, unless this has been clearly rejected by the
patient.
By the end of the study it is possible for participants to contact the study coordinator to
get information about study results.
Method All source data from the study will be stored according to Danish legislation and the
study will be approved by the Danish Data Protection Agency.
Lumbar puncture and Serum The participation implies extraction 2-3 ml of spinal fluid and a
blood sample about 10 ml, which will be used for analysis of sCD163. Spinal fluid and blood
samples will be drawn in the same procedure as the diagnostic samples. For the GBS patients
a repeat blood test will be performed after 2-4 weeks and again after 6 months. The
extraction of blood sample will be about 10 ml each repeated test. A repeated lumbar
puncture will be performed after 6 months as well, where the extraction amount will also be
about 2-3ml of spinal fluid. The patients have full right to say no to the repeated
procedure. The symptomatic controls will only have spinal fluid and blood samples drawn
once.
Other examinations
Patients with GBS will undergo a clinical examination with the following the neuropathy
scores:
Participants will be evaluated clinically with: Neuropathy Impairment Score (NIS):
The NIS is a composite clinical scoring system that has been widely used to objectively
assess the severity of peripheral neuropathy. The NIS quantifies the findings of the
neurologic examination by attributing a score (ranging from 0 [normal] to 88 [total
impairment]) to the clinical abnormalities noted in the physical assessment of sensation,
muscle power, and tendon reflexes.
Overall Disability Sum Score (ODSS):
The ODSS seems to be valid and sensible measure to score dysfunction and disability in
patients with motor neuropathies. This score is composed of arm and leg disability scale
with a total score ranging from 0 (no signs of disability) to 12 (most severe disability
score). The patient and the observer will judge each activity with: not affected, affected
and not prevented or prevented.
Analyses The spinal fluid and the serum will be analysed at the Medical Research Laboratory.
The spinal fluid and blood samples will be deposited in a research biobank at Aarhus
University Hospital. The plan is to carry out all the biochemical analyses at the same time
when all samples are collected. Any excess biological material will be transferred to a
biobank for future research.
Serum and CSF will be analyzed for sCD163 and other inflammatory markers, by already
established techniques at the Department of Clinical Medicine, Aarhus University Hospital
and the spinal fluid will be examined with spectrometry.
Statistics GBS is a rare disease and because of the low number of patients, it is difficult
to perform power calculations in studies of GBS patients. However, the investigators
anticipate that 20 patients and 40 controls will be sufficient.
The program STATA will be used for statistical analyses. Parametric or non- parametric tests
will be used depending of the normal distribution of data. In all statistical analysis a 5%
level of significance will be used.
Ethical considerations The local Ethics Committee of the Central Region of Denmark will
approve the study.
The tests performed on the patients involved in this research project are lumbar puncture,
blood sampling and clinical examination.
A lumbar puncture is a procedure used to collect cerebral spinal fluid (CSF) for analysis.
CSF is a promising source of biomarkers in GBS since the proximal nerve roots, which are
affected early in the disease, are in close anatomical contact with the CSF space.
During the procedure a hollow needle is used to penetrate the spinal canal at the level of
the third- to-fourth or fourth-to-fifth lumbar vertebra in the low back. CSF then drips out
through the needle and is collected. It involves some risk to perform a lumbar puncture,
which include post lumbar puncture headache with an incidence of 12%, most common in younger
patients. Most frequently, the headache has a short duration and it may be cured with
application of a blood -patch. Moreover, lumbar puncture can cause temporary lumbar pain and
an accumulation of blood underneath the skin. There is a very low risk of bleeding in the
epidural space with compression of spinal nerves and an even lower risk of infections
(meningitis or meningoencephalitis).
However, it is important to highlight that lumbar puncture will be performed as a routine
diagnostic for GBS patients; therefore it will not cause the participants any further
problem.
The investigators consider this project to be ethically acceptable considering of the low
risk involved in this project, the seriousness and costs of the disease and the potential to
identify a relevant biomarker in GBS.
At all times during the diagnostic work-up and consequently in the research project
investigators will do this with respect for the patients mental and physical condition. The
research project will be carried out according to the Act on Processing of Personal Data
and, furthermore, the project will be submitted to the common regional information system
for Region Midtjylland Publications Positive, negative or inconclusive results will be
published in an international journal with Malalai Musleh as first author, Professor Henning
Andersen as senior author and Lars Markvardsen will be co-author.
