Does Notch Regulate Glioma Proliferation ?

Glioma Clinical Trial

— GLIONOTCH  

Official title:

Study of the Activity and Role of Notch1 Signalling in Glioma

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04809597
• Other study ID #: RECHMPL21_0172
• Status: Completed
• Start date: January 1, 2012
• Est. completion date: January 20, 2021

Study information

• Verified date: March 2021
• Source: University Hospital, Montpellier
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The investigators showed in 2015 (Guichet et al) that the Notch1 pathway has an anti-proliferative role on glial brain tumors. In this project, the investigators investigated which genes downstream of Notch1 pathway activation produce the anti-proliferative effect. The investigators hypothesized that the SNAI2 and TAL1 genes act downstream of Notch1

Description:

Main objectives:

To examine the expression of TAL1 and SNAI2 in protein extracts and glioma sections of different grades (glioblastomas and diffuse low-grade tumors).

To examine the effect of overexpression of Tal1 and Snai2 in high grade glioma cultures.

To show the expression of Tal1 and SNAI2 by tumor cells by FISH technique. Show that TAL1 and SNAI2 could be regulated by endothelial cells

Recruitment information / eligibility

• Status: Completed
• Enrollment: 10
• Est. completion date: January 20, 2021
• Est. primary completion date: January 1, 2021
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion criteria:

• Patient =18 years old

• Patient affected with brain glioma classified according to WHO 2016 classification

Exclusion Criteria:

• Patient who reject the study protocol

• Radiotheray or chemotherapy treatment

Related Conditions & MeSH terms

• Glioma

Locations

Country	Name	City	State
France	Uhmontpellier	Montpellier

Sponsors (1)

Lead Sponsor	Collaborator
University Hospital, Montpellier

Country where clinical trial is conducted

France, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Effect of Notch1 activation on Tal1	highthroughput gene analysis (DNA arrays) with cells transduced with activated form of Notch1	1 day
Primary	Snai2 gene and protein expression	western blot for Tal1 and Snai2 in protein extracted from low grade and high gliomas	1 day