Molecular Taxonomy of Surgically-harvested Ocular Tissues Defined by Single-cell Transcriptomics

Glaucoma Clinical Trial

— Eyesinglecell  

Official title:

Molecular Taxonomy of Surgically-harvested Ocular Tissues Defined by Single-cell Transcriptomics

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT04682054
• Other study ID #: Eyesinglecell
• Status: Not yet recruiting
• Phase: N/A
• Start date: January 15, 2021
• Est. completion date: December 31, 2021

Study information

• Verified date: December 2020
• Source: The First Affiliated Hospital with Nanjing Medical University
• Contact: Zizong Hu, PhD, MD
• Phone: +8615195960100
• Email: huzizhong[@]njum.edu.cn
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The surgically-harvested eye tissue (ie. vitreous-retinal proliferative membrane, outflow pathway, retinas, and pterygium) is a complex tissue responsible for maintaining intraocular homeostasis or mediating ocular pathogenesis. Dysfunction of one or more resident cell types within the tissues results in different ocular disorder, leading to vision loss, or even blindness. In this study, we aim to use single-cell RNA sequencing to generate a comprehensive cell atlas of surgically-harvested eye tissues.

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 80
• Est. completion date: December 31, 2021
• Est. primary completion date: December 31, 2021
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 90 Years

Eligibility

Inclusion Criteria:

1. Patients with ocular diseases and require surgery intervention.

2. The surgery intervention remove pathological tissue during surgery.

3. Patients consent to the treatment and single-cell sequencing.

Exclusion Criteria:

1. Surgically removed eye tissue cannot meet with the quality control of single-cell sequencing (mainly the tissue weight and cell counts);

Related Conditions & MeSH terms

• Diabetic Retinopathy
• Glaucoma
• Proliferative Vitreoretinopathy
• Pterygium

Intervention

Procedure:
• ocular surgery
surgery to remove the pathological eye tissue

Locations

Country	Name	City	State
China	First Affiliated Hospital of Nanjing Medical University	Nanjing	Jiangsu

Sponsors (1)

Lead Sponsor	Collaborator
The First Affiliated Hospital with Nanjing Medical University

Country where clinical trial is conducted

China, 

References & Publications (4)

de Bruijn SE, Fiorentino A, Ottaviani D, Fanucchi S, Melo US, Corral-Serrano JC, Mulders T, Georgiou M, Rivolta C, Pontikos N, Arno G, Roberts L, Greenberg J, Albert S, Gilissen C, Aben M, Rebello G, Mead S, Raymond FL, Corominas J, Smith CEL, Kremer H, Downes S, Black GC, Webster AR, Inglehearn CF, van den Born LI, Koenekoop RK, Michaelides M, Ramesar RS, Hoyng CB, Mundlos S, Mhlanga MM, Cremers FPM, Cheetham ME, Roosing S, Hardcastle AJ. Structural Variants Create New Topological-Associated Domains and Ectopic Retinal Enhancer-Gene Contact in Dominant Retinitis Pigmentosa. Am J Hum Genet. 2020 Nov 5;107(5):802-814. doi: 10.1016/j.ajhg.2020.09.002. Epub 2020 Oct 5. — View Citation

Tan WJ, Meng MW, Ricciardi-Castagnoli P, Tang T, Yee ACS, Lim TS. Single B-cell Genomic Analyses Differentiates Vitreoretinal Lymphoma from Chronic Inflammation. Ophthalmology. 2020 Nov 19. pii: S0161-6420(20)31110-6. doi: 10.1016/j.ophtha.2020.11.018. [Epub ahead of print] — View Citation

van Zyl T, Yan W, McAdams A, Peng YR, Shekhar K, Regev A, Juric D, Sanes JR. Cell atlas of aqueous humor outflow pathways in eyes of humans and four model species provides insight into glaucoma pathogenesis. Proc Natl Acad Sci U S A. 2020 May 12;117(19):10339-10349. doi: 10.1073/pnas.2001250117. Epub 2020 Apr 27. — View Citation

Wynne N, Carroll J, Duncan JL. Promises and pitfalls of evaluating retinal diseases with adaptive optics scanning light ophthalmoscopy (AOSLO). Prog Retin Eye Res. 2020 Nov 5:100920. doi: 10.1016/j.preteyeres.2020.100920. [Epub ahead of print] Review. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	counts of each cell type	single-cell sequencing identify what cells and the counts within the eye tissue	1 month