Serological Evaluation of 77A/G and 11A/12A Gene of MMP-13 in Periodontitis Affected and Healthy Individuals

Generalized Adult Periodontitis Clinical Trial

— SEGPPAH  

Official title:

A Serological Evaluation of 77A/G and 11A/12A Associated Gene Polymorphisms of MMP-13 in Periodontitis Affected and Healthy Individuals

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT02423304
• Other study ID #: Divya Aiswairya thesis
• Status: Not yet recruiting
• Phase: N/A
• First received: April 17, 2015
• Last updated: April 21, 2015
• Start date: May 2015
• Est. completion date: June 2016

Study information

• Verified date: April 2015
• Source: Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre
• Contact: n/a
• Is FDA regulated: No
• Health authority: India: Institutional Review Board
• Study type: Observational

Clinical Trial Summary

50 periodontitis 50 healthy individuals serological evaluation will done to see association of 77A/G AND 11A/12A gene polymorphisms of MMP-13

Description:

• The study will be conducted on 100 individuals

• 50 chronic periodontitis and 50 healthy individuals will be recruited from the Out Patient Department of Periodontics in the study.

• An informed consent will be obtained from patients who wish to participate in the study.

• Patients will be categorized by Periodontal Disease Index (PDI) by P. Ramfjord(1959)

• After clinical examination, blood samples will be collected and assessed for MMP 13 Gene Polymorphisms.

• Subjects will be selected according to the study criteria and divided into two groups (50 each).

1. Test group

2. Control group

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 100
• Est. completion date: June 2016
• Est. primary completion date: May 2016
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Both
• Age group: 30 Years to 55 Years

Eligibility

Inclusion Criteria:• Test group

1. Patients with chronic periodontitis classified according to American Academy of Periodontology ( AAP) 1999

2. Subjects aged between 30-55 years

• Control group Systemically and periodontally healthy individuals of same age group

Exclusion Criteria:

1. Present and past Smokers

2. Presence of any systemic diseases.

3. Oral diseases other than Periodontitis

4. With a previous history of periodontal treatment in the past 6 months and subjects under any medications.

5. Family history of any diagnosed genetic disorders.

Study Design

Observational Model: Cohort, Time Perspective: Cross-Sectional

Related Conditions & MeSH terms

• Chronic Periodontitis
• Generalized Adult Periodontitis
• Periodontitis

Intervention

Genetic:
• blood samples collected from all patients
blood samples collected from brachial pluxes

Locations

Country	Name	City	State
n/a

Sponsors (2)

Lead Sponsor	Collaborator
Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre	Research Institute for Genetic and Human Therapy

References & Publications (4)

Hernández Ríos M, Sorsa T, Obregón F, Tervahartiala T, Valenzuela MA, Pozo P, Dutzan N, Lesaffre E, Molas M, Gamonal J. Proteolytic roles of matrix metalloproteinase (MMP)-13 during progression of chronic periodontitis: initial evidence for MMP-13/MMP-9 a — View Citation

Kiili M, Cox SW, Chen HY, Wahlgren J, Maisi P, Eley BM, Salo T, Sorsa T. Collagenase-2 (MMP-8) and collagenase-3 (MMP-13) in adult periodontitis: molecular forms and levels in gingival crevicular fluid and immunolocalisation in gingival tissue. J Clin Per — View Citation

Pirhan D, Atilla G, Emingil G, Tervahartiala T, Sorsa T, Berdeli A. MMP-13 promoter polymorphisms in patients with chronic periodontitis: effects on GCF MMP-13 levels and outcome of periodontal therapy. J Clin Periodontol. 2009 Jun;36(6):474-81. doi: 10.1111/j.1600-051X.2009.01399.x. Epub 2009 Apr 22. — View Citation

Uitto VJ, Airola K, Vaalamo M, Johansson N, Putnins EE, Firth JD, Salonen J, López-Otín C, Saarialho-Kere U, Kähäri VM. Collagenase-3 (matrix metalloproteinase-13) expression is induced in oral mucosal epithelium during chronic inflammation. Am J Pathol. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	to evaluate the association of 77A/G AND 11A/12A gene polymorphism of mmp-13 in periodontitis effected and healthy individuals	collect DNA samples from serum and evaluate for gene polymorphism	6 months	Yes