Effect of 2000-meter Rowing Test on Parameters of Intestinal Integrity in Elite Rowers During Competitive Phase

Endothelial Dysfunction Clinical Trial

— Rowers  

Official title:

Effect of 2000-meter Rowing Test on Parameters of Intestinal Integrity in Elite Rowers During Competitive Phase

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT06372262
• Other study ID #: Rowers2022
• Status: Completed
• Start date: June 1, 2022
• Est. completion date: June 30, 2022

Study information

• Verified date: June 2022
• Source: Poznan University of Physical Education
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The study aimed to check the 2000m ergometer test on markers of gut permeability in the competitive phase of rowers. 18 members of the Polish rowing team took part in the study.

Description:

The phenomenon of epithelial wall leakage has been the subject of investigations within athletic domains such as running and cycling. Nevertheless, there exists an insufficiency of understanding regarding gut permeability in alternative disciplines, such as rowing, particularly following the completion of a standard competitive distance of 2000 meters. Hence, the principal objective of our study was to assess gut permeability after completing a 2000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 2000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1-hour of restitution. Parameters, such as I-FABP, LPS, LBP, and zonulin, were determined using appropriate biochemical tests.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 18
• Est. completion date: June 30, 2022
• Est. primary completion date: June 30, 2022
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 23 Years

Eligibility

Inclusion Criteria:

• minimum five years of training,
• five training sessions per week minimum,
• total training time minimum of 240 minutes,
• membership in the Polish Rowing Team,
• finishing the 2000-meter ergometer test.

Exclusion Criteria:

• antibiotic therapy within the last three months,
• probiotics and prebiotics within the last three months,
• dietary regime,
• gastrointestinal diseases.

Related Conditions & MeSH terms

• Endothelial Dysfunction

Intervention

Other:
• ergometer test
2000meter rowing ergometer test

Locations

Country	Name	City	State
Poland	Poznan University of Physical Education	Poznan

Sponsors (1)

Lead Sponsor	Collaborator
Poznan University of Physical Education

Country where clinical trial is conducted

Poland, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury	I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Primary	zonulin to measure tight junction leakage	zonulin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Primary	LBP (lipopolysaccharide binding protein) to measure endotoxin	LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Primary	LPS (lipopolysaccharide) to measure endotoxin	LPS concentration measured in blood [EU/Ll] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Primary	Lactic acid to measure fatigue after the race	Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany)	Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
Secondary	energy	energy [kcal ]of food intake measured by 24h dietary recall	day before test
Secondary	fiber intake	fiber [g] intake day before the test measured by 24h dietary recall	day before test
Secondary	protein	protein [g] intake day before the test measured by 24h dietary recall	day before test
Secondary	carbohydrate	carbohydrate [g] intake day before the test measured by 24h dietary recall	day before test
Secondary	body mass	Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Secondary	Body composition - water	% of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Secondary	Body composition - fat	% of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test
Secondary	Lean body mass	Lean body mass [kg] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test