An Investigation Into the Effects of Blueberry Anthocyanin on Cognitive Functioning and Mood in Adolescents.

Depression Clinical Trial

Official title:

A Randomized, Double-blind, Placebo-controlled Study Investigating the Effects of Blueberry Anthocyanin on Cognitive Functioning and Mood in Adolescents.

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03119597
• Other study ID #: RDG002
• Status: Completed
• Phase: N/A
• Start date: January 12, 2017
• Est. completion date: December 22, 2018

Study information

• Verified date: May 2023
• Source: University of Reading
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of this study is to determine whether a 4-week dietary intervention with wild blueberry powder can have an effect on cognitive performance in participants aged between 13-17.

Description:

Interventions: Flavonoid drink and a matched placebo will be used in this study. Flavonoid drink will contain approximately, 13g of freeze dried wild blueberry powder, containing 250mg of anthocyanin flavonoid, mixed with 170ml water and flavored with 30ml 'Rock's Organic Orange Squash' as it has a low flavonoid content. The placebo drink will contain placebo powder matched for vitamin C (45mg), glucose (4.52g) and fructose (4.79g), mixed with 170ml of water and 30ml 'Rock's Organic Orange Squash'. Participants will be randomly allocated to either placebo or treatment. Participants will be instructed to consume the drink every morning for 4 weeks. The powder will be prepared and sealed in opaque packets. Participants will be provided with 2 weeks worth of supplies at baseline and then in week 2. Parents will be provided with instructions on how to prepare the drinks in addition to supplies such as opaque straws and containers. Assessment of left-over product will be conducted in 2 weeks to allow for continued compliance verification.

Procedure: the study comprises of a screen and four visits, including a familiarization week and 2 test visits.

1. Screening: Once a school has indicated interest in aiding with this trial, participants will be given a brief overview of the study and asked to complete a set of questionnaires regarding their mood and general health to confirm suitability for participating in this study. The questionnaires include Mood and Feeling Questionnaire (MFQ) and Revised Child Anxiety and Depression Scale (RCADS).Participants who score 27 or above (depressed) and score 14 or below (healthy) on the MFQ will be invited to participate in the second part of the study. Parents of the participants meeting the criteria will then be contacted to ascertain that each interested volunteer and parents are willing to participate for the entire duration of the study and to schedule their familiarization visit.

2. Familiarization Visit: Volunteers and parents will be invited to volunteers' first visit at the location of volunteers' choice (Nutritional Psychology Unit at the University of Reading or volunteers' school) where participants will receive a detailed explanation of the study by the participating research associate and will be asked to sign the informed consent form before any study procedure starts. A log will be kept identifying all participants having signed the informed consent form (ICF) and the participant will be allocated a participant number. Once consent has been given, the participants will then be asked to complete the EPIC Food Frequency Questionnaire so that background flavonoid intakes can be measured prior to the start of the intervention, Rumination Response Scale (RRS) and a General health questionnaire. Participants intelligence quotient (IQ) will be assessed using The Wechsler Abbreviated Scale of Intelligence (WASIII). Parents will also be asked to complete questionnaires, regarding children mood and behaviors, including the Parent version of MFQ, RCADS and attention deficit hyperactivity disorder (ADHD) Rating Scale-IV. Finally, participants will be given training on the cognitive test battery (tasks outlined below) that will be used during the study to reduce the chances of 'practice' adversely affecting performance on subsequent test days.

3. Test Visits (Weeks 0, 2 and 4): All of these sessions will be held at times that suit participants' individual needs. The timings of the testing sessions will be consistent for each participant over the 4 weeks. Upon arrival, participants will be asked of any adverse events and any issues with compliance. Participants will then complete the battery of cognitive tasks consisting of (i) Modified Eriksen Flanker task, (ii) The Keep track task (KTT) and (iii)The Controlled Oral Word Association Test (COWAT), in addition to completing the daily mood measure Positive and Negative Affect Schedule (PANAS). Additionally, the last test session (week 4) participants and parents' will once again be asked to complete the MFQ, RCADS and RRS mentioned above.

Participants may stop the study treatment without stating any reason at any time during the study. In addition, a participant will be withdrawn from the study if a volunteer or parents request a discontinuation, participant exhibits a serious adverse event to any component of the test product, an illness emerges and/or the investigator's opinion is that withdrawal is appropriate and in the best interest of the participant. If a participant withdraws from the study, participants will be encouraged to make a final visit as soon as possible, irrespective of the reason for withdrawal, to complete a final battery of cognitive tasks.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 66
• Est. completion date: December 22, 2018
• Est. primary completion date: November 27, 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 13 Years to 17 Years

Eligibility

Inclusion Criteria:

• Overall healthy volunteers as proven by medical history and/or physical examination and of all ethnicity and socioeconomic status

• Participants at risk of depression or healthy as assessed by MFQ (score 27 or above and score 14 or below)

• Able to get a parent or guardian to give signed written informed consent in addition to they themselves giving informed assent

Exclusion Criteria:

• Use of complementary and alternative medicine

• History of metabolic disorder, diabetes, or other medical conditions.

• Participants using medications that might affect the outcome measures, such as antidepressant and sleeping medication.

• Participants who score between 14 and 27 on the MFQ.

Related Conditions & MeSH terms

• Depression

Intervention

Dietary Supplement:
• Wild Blueberry Powder-13g
Formulation containing approximately 250mg of anthocyanin+ 4.79g Fructose+ 4.52g Glucose+ 45mg Vitamin C
• Placebo
Formulation containing approximately 4.79g Fructose+ 4.52g Glucose+ 45mg Vitamin C

Locations

Country	Name	City	State
United Kingdom	University of Reading	Reading	Berkshire

Sponsors (2)

Lead Sponsor	Collaborator
University of Reading	Wild Blueberry Association of North America (WBANA)

Country where clinical trial is conducted

United Kingdom, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in Working Memory performance	Keep tract task: measuring working memory performance	0, 2 and 4 weeks
Primary	Change in Mood	measured by use of PANAS	0, 2 and 4 weeks
Primary	Change in verbal fluency	Controlled oral word association task: measuring working memory performance	0, 2 and 4 weeks
Primary	Change in Cognitive Accuracy	Modified Flanker Task: measuring accuracy	0, 2 and 4 weeks
Primary	Change in reaction time	Modified Flanker Task: measuring reaction time	0, 2 and 4 weeks
Primary	Change in Depression	measured by use of MFQ	0 and 4 weeks
Primary	Change in Anxiety	measured by use of RCADS	0 and 4 weeks
Secondary	Change in Rumination	Measured using Rumination Response Scale.	0 and 4 week