Romanian Kidney Transplant Cardiovascular Risk Registry

Cardiovascular Diseases Clinical Trial

— ROKET-CV  

Official title:

The Complex Evaluation of the Cardiovascular Risk in the Kidney Transplant Patients

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT04451356
• Other study ID #: UMF
• Status: Recruiting
• Start date: February 1, 2020
• Est. completion date: December 2022

Study information

• Verified date: June 2020
• Source: Grigore T. Popa University of Medicine and Pharmacy
• Contact: Dimitrie Siriopol
• Phone: +40740138874
• Email: dimitrie.siriopol[@]yahoo.com
• Is FDA regulated: No
• Study type: Observational [Patient Registry]

Clinical Trial Summary

Kidney transplantation (KT) represents the best treatment for patients with end-stage kidney disease, being associated with improved outcomes and reduced mortality. Although the survival benefit with KT is mostly attributable to reduction in cardiovascular (CV) disease, KT recipients continue to remain at higher risk for CV-related morbidity and mortality when compared with the general population. Additionally, CV events represent the leading cause of death in KT recipients with a functioning allograft. KT recipients have high rates of hospitalization for myocardial infarction, congestive heart failure, dysrhythmias, stroke, malignant hypertension, and cardiac arrest. Significant amounts of research have been aimed at reducing event rates, primarily aimed at understanding prevalent risk factors, defining outcomes, and application of guideline-based care.

The post-KT milieu represents the confluence of several traditional and nontraditional CV risk factors contributing to the significant CV risk in this population. CV disease remains an understudied and undertreated source of morbidity and mortality in KT patients. Patients with chronic kidney disease (CKD) are generally excluded from major cardiovascular outcome trials, and this phenomenon of aversion to including patients with CKD in CV trials and providing appropriate goal-directed medical and interventional therapies (renalism) extends into KT .

The main aim of this study is to evaluate holistically the CV risk in a KT population. The investigators will compare bioimpedance spectroscopy derived fluid status parameters (overhydration, total body water, extracellular water and intracellular water) with clinical evaluation, lung ultrasonography, pulse wave velocity, different biomarkers, and echocardiographic characteristics and also to determine the impact of these parameters on renal and CV outcomes in the same population.

Description:

• Patients:

The inclusion criteria are:

1. age>18 years;

2. KT recipient.

The exclusion criteria are:

1. metallic joint prostheses, cardiac stent or pacemakers, decompensated cirrhosis, pregnancy and limb amputations (due to bioimpedance technique limitations);

2. no prior diagnosis of pulmonary fibrosis, pneumectomy or massive pleural effusion (due to lung ultrasonography limitations);

3. active systemic infections (due to difficulties in the interpretation of nespecific inflammation biomarkers in this type of patients);

4. absence of congenital heart disease;

5. eGFR below 30 ml/min/1.73m2;

6. KT vintage of at least 6 months.

• Methods:

All patients will have a complete clinical and paraclinical evaluation:

• Personal data (age, sex, height);

• Cardiovascular risk factors (smoking, weight, body mass index);

• Medication (including immunosupression);

• Kidney transplantation data (date of transplantation, type - living or cadaveric donor, missmatch number, type of induction therapy);

• Dialysis vintage (before KT)

• Comorbidities (diabetes, hypertension, stroke, coronary artery disease, etc);

• Physical examination (blood pressure, crackles, pedal edema etc);

• Renal function evaluation (serum urea and creatinine, serum electrolytes - K, Na, Cl)

• Nutrition status evaluation: serum protein, cholesterol, triglycerides;

• Inflammation evaluation: CRP;

• Serum glucose and uric acid;

• Hemoleucogram;

• Electrocardiogram.

Bioimpedance spectroscopy analysis This analysis will be performed at baseline using the portable whole-body multifrequency bioimpedance analysis device (BCM®Body Composition Monitor

• Fresenius Medical Care D GmbH). The technique involves attaching electrodes to the patient's forearm and ipsilateral ankle, with the patient in a supine position. The BCM® measures the body resistance and reactance to electrical currents of 50 discrete frequencies, ranging between 5 and 1000 kHz. Based on a fluid model using these resistances, the extracellular water (ECW), the intracellular water (ICW) and the total body water (TBW) are calculated. These volumes are then used to determine the amount of fluid overload. All calculations are automatically performed by the software of the BCM® device. Absolute fluid overload (AFO) is is defined as the difference between the expected patient's ECW under normal physiological conditions and the actual ECW, whereas the relative fluid overload (RFO) is defined as the absolute fluid overload AFO to ECW ratio. Normohydration is defined when AFO is between the 10th and the 90th percentile for healthy, age- and gender-matched individuals from the reference population, i.e. between -1.1 to +1.1 L, while volumes below and above this range defining underhydration and overhydration, respectively. In addition, this analysis will provide the LTI and FTI for the included patients.

Echocardiography Echocardiographic evaluations will be made in each patient at baseline. All echocardiographic measurements will be carried out according to the recommendations of the American Society of Echocardiography by an observer unaware of the lung ultrasound and bioimpedance results. Echocardiographic evaluation will provide information about cardiac anatomy (e.g. volumes, geometry, mass) and function (e.g. left ventricular function and wall motion, valvular function, right ventricular function, pulmonary artery pressure, pericardium).

Lung ultrasonography Examinations will be performed in the supine position. Scanning of the anterior and lateral chest will be performed on both sides of the chest, from the second to the fourth (on the right side to the fifth) intercostal spaces, at parasternal to mid-axillary lines. B-lines will be recorded in each intercostal space and were defined as a hyperechoic, coherent US bundle at narrow basis going from the transducer to the limit of the screen. B-lines starting from the pleural line can be either localized or scattered to the whole lung and be present as isolated or multiple artifacts. The sum of B-lines produces a score reflecting the extent of lung water accumulation (0 being no detectable B-line).

Arterial stiffness Arterial stiffness assessment will be performed by applanation tonometry (SphygmoCorTM; PWV Inc., Westmead, Sydney, Australia) with the patient being recumbent, 10 minutes before the measures were done. The carotid and femoral pulse will be acquired by applanation tonometry sequentially, allowing a single operator to acquire the measurement. The transit time from the R-wave of the simultaneously acquired electrocardiogram to the foot of the carotid and femoral pulse is measured. The difference acquired electrocardiogram to the foot of the carotid and femoral pulse is measured. The difference between these 2 transit times is divided by distances measured from the body surface to estimate the arterial path length in order to calculate carotid-femoral PWV. The SphygmoCor PWV has been established in the literature as a reference for comparison due to the wealth of population data and healthy population refence values. The investigators will determine two PWVs for every patient and use the media for the statistical analysis. If the difference between these two measurements will be higher than 0.5 m/s then another determination will be performed and the median will be used for analysis.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 300
• Est. completion date: December 2022
• Est. primary completion date: December 2022
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 75 Years

Eligibility

Inclusion Criteria:

1. age>18 years;

2. Kidney Transplant Recipient.

Exclusion Criteria:

1. metallic joint prostheses, cardiac stent or pacemakers, decompensated cirrhosis, pregnancy and limb amputations (due to bioimpedance technique limitations);

2. no prior diagnosis of pulmonary fibrosis, pneumectomy or massive pleural effusion (due to lung ultrasonography limitations);

3. active systemic infections (due to difficulties in the interpretation of nespecific inflammation biomarkers in this type of patients);

4. absence of congenital heart disease;

5. eGFR below 30 ml/min/1.73m2;

6. KT vintage of at least 6 months.

Related Conditions & MeSH terms

• Cardiovascular Diseases
• Kidney Transplant; Complications

Intervention

Device:
• Bioimpedance Spectroscopy
This analysis will be performed at baseline using the portable whole-body multifrequency bioimpedance analysis device (BCM®Body Composition Monitor - Fresenius Medical Care D GmbH). The technique involves attaching electrodes to the patient's forearm and ipsilateral ankle, with the patient in a supine position. The BCM® measures the body resistance and reactance to electrical currents of 50 discrete frequencies, ranging between 5 and 1000 kHz. Based on a fluid model using these resistances, the extracellular water (ECW), the intracellular water (ICW) and the total body water (TBW) are calculated. These volumes are then used to determine the amount of fluid overload. All calculations are automatically performed by the software of the BCM® device. In addition, this analysis will provide the LTI and FTI for the included patients.
• Arterial Stiffness
Arterial stiffness assessment will be performed by applanation tonometry (SphygmoCorTM; PWV Inc., Westmead, Sydney, Australia) with the patient being recumbent, 10 minutes before the measures were done. The carotid and femoral pulse will be acquired by applanation tonometry sequentially, allowing a single operator to acquire the measurement. The transit time from the R-wave of the simultaneously acquired electrocardiogram to the foot of the carotid and femoral pulse is measured. The difference acquired electrocardiogram to the foot of the carotid and femoral pulse is measured. The difference between these 2 transit times is divided by distances measured from the body surface to estimate the arterial path length in order to calculate carotid-femoral PWV.

Diagnostic Test:
• Echocardiography
Echocardiographic evaluations will be made in each patient at baseline. All echocardiographic measurements will be carried out according to the recommendations of the American Society of Echocardiography by an observer unaware of the lung ultrasound and bioimpedance results. Echocardiographic evaluation will provide information about cardiac anatomy (e.g. volumes, geometry, mass) and function (e.g. left ventricular function and wall motion, valvular function, right ventricular function, pulmonary artery pressure, pericardium).
• Lung ultrasonography
Examinations will be performed in the supine position. Scanning of the anterior and lateral chest will be performed on both sides of the chest, from the second to the fourth (on the right side to the fifth) intercostal spaces, at parasternal to mid-axillary lines. B-lines will be recorded in each intercostal space and were defined as a hyperechoic, coherent US bundle at narrow basis going from the transducer to the limit of the screen. B-lines starting from the pleural line can be either localized or scattered to the whole lung and be present as isolated or multiple artifacts. The sum of B-lines produces a score reflecting the extent of lung water accumulation (0 being no detectable B-line).

Locations

Country	Name	City	State
Romania	"Dr. C.I. Parhon" Hospital	Iasi

Sponsors (1)

Lead Sponsor	Collaborator
Grigore T. Popa University of Medicine and Pharmacy

Country where clinical trial is conducted

Romania, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Composite Renal Outcome	Doubling of creatinine or dialysis initiation.	36 months
Primary	Composite Cardiovascular Outcome	Time to first non-fatal myocardial infarction, non-fatal stroke, hospitalization for heart failure, or cardiovascular death	36 months
Secondary	Chronic kidney disease progression	The impact of baseline characteristics on eGFR (as assessed by the CKD-EPI formula) evolution	36 months
Secondary	Proteinuria progression	The impact of baseline characteristics on proteinuria (as assessed by the urine protein to creatinine ratio) evolution	36 months