Effect of Bromelain Versus Calcium Hydroxide as an Intracanal Medication on the Intensity of Postoperative Pain and Bacterial Load Reduction in Necrotic Single Rooted Lower Premolars

Calcium Hydroxide Clinical Trial

Official title: Effect of Bromelain Versus Calcium Hydroxide as an Intracanal Medication on the Intensity of Postoperative Pain and Bacterial Load Reduction in Necrotic Single Rooted Lower Premolars (A Randomized Clinical Trial)

Status Not yet recruiting

Clinical Trial Summary

the aim of the study is to compare the effect of bromelain vs calcium hydroxide as intracanal medication on intensity of pain and bacterial load in necrotic single lower premolars

Clinical Trial Description

After diagnosing the case as pulp necrosis and confirming that the patient conforms to all eligibility criteria, the operator will enroll the patient in the study.

Treatment of all cases will be completed in two visits as follows :

1. Anesthetizing the tooth using mental nerve block technique by local anesthesia of 1.8 ml of 2% Mepivacaine HCl with 1:100,000 epinephrine (Artinibsa®; Inibsa Dental, Lliçà de Vall,Spain) .

2. Removal of Caries and/or coronal restorations completely with sterile bur and rubber dam will be applied.

3. Cleaning the operative field, including the tooth, the clamp, and rubber dam sheet, with 3% hydrogen peroxide until no further bubbling of the peroxide occurred. All surfaces will then be disinfected by a sterile cotton swab with a 5.25% sodium hypochlorite solution.

4. Preparation of access cavity using another sterile round carbide bur size 3 and Endo-z bur (Dentsply Maillefer, Ballaigues, Switzerland) .

5. After completing the access, the operative field and the pulp chamber will be cleaned and disinfected once again in the same way mentioned above. NaOCl will be then neutralized with 5% sodium thiosulfate.

6. The pre-instrumentation root canal sample (S0) will be taken as follows: a sterile paper point will be placed in the canal to soak up the fluid in the canal to a level approximately 1 mm short of the tooth apex based on diagnostic radiographs and estimated working length. The paper point will be left in the canal for at least 1 minute. Paper points will then be transferred aseptically to tubes containing sterile brain heart infusion (BHI) broth.

7. Confirming the patency of the root canals using stainless steel hand K-files size #10 and #15(K-Files, MANI, INC., Industrial Park, Utsunomiya, Tochigi, Japan) Working length will be determined using an electronic apex locator(Root ZX, J. Morita USA, Irvine, CA) then confirmed radiographically to be 1 mm shorter than radiographic apex.

8. Mechanical preparation will be done using M PRO rotary files in an endodontic motor (X-Smart, Dentsply Maillefer, USA) The first file (18/.09) will be used as an orifice opener for two thirds of the working length followed by (20/.04) and (25/.06) for the full working length and finally (35/.04). In-and-out motions will be applied with stroke lengths not exceeding 3 mm in the cervical, middle, and apical thirds until attaining the established WL. The first file is used with a continuous rotary motion at a speed of 500rpm and torque of 3Ncm. The second, third and fourth files are used with a speed of 300 rpm and torque of 1.5Ncm. The canal will be irrigated and recapitulated after the use of each instrument.

9. The canal will be thoroughly irrigated with 2.5% sodium hypochlorite root canal irrigant (5ml for 1 min) using disposable plastic syringe with side vented needle gauge 30 (Steri irrigation tips; Diadent, Chungcheongbuk-do, Korea) reaching 1 mm short of the working length. All teeth will receive the same volume of irrigant (5 ml prior to instrumentation, 5 ml between each file and 5 ml as final flush after root canal instrumentation to reach a total volume of 25 ml in total).

10. Post-instrumentation sample (S1) will be taken as previously mentioned.

11. The patients will then be assigned into two groups :

In the intervention groups, Bromelain paste (Bromelain powder with enzymatic activity of 2400 Gelatin digestion unit per Gram was mixed with saline in 1:1 proportion 1 g powder was mixed with 1 ml distilled water) will be placed inside the canals using sterile plastic syringe with needle gauge 27 without binding 1mm shorter than working length . The access cavities were closed using sterile cotton pellet and intermediate restorative material .

In the control group, Ca(OH)29will be placed inside the canals using sterile plastic syringe with needle gauge 27 without binding 1 mm shorter than working length .The access cavities were closed using sterile cotton pellet and intermediate restorative material .

12. Patients will be asked to record their pain level after the first visit on Numeric Rating Scale (NRS) at 6, 12, 24, 48 hours.(Appendix V)

13. Each patient will be given a chart to record postoperative swelling. If swelling was recorded, the patient will be appointed for clinical examination to assess the severity by blinded assessor in a swelling rating scale and to determine if systemic antibiotics (Augmentin 625mg/8 hours/5 days) or drainage would have been needed.

14. In the recall appointment after 1 week. Rubber dam will be applied then intracanal medication will be removed using copious irrigation and fanta AF max rotary file (#25 0.04) and tooth will be disinfected as before. The previously sampled canal will be re-entered, flushed with copious saline irrigation and a third sample (S-2) will be taken. After the sampling, final flush with 2.5% NaOCL and 17% EDTA will be done in both groups. Master cones of (0.40) taper gutta-percha(Meta Biomed Co., Ltd, Korea) will be fitted to the working length and a radiograph will be taken to ensure proper length. Obturation will be done by modified single cone technique using epoxy resin sealer(Adseal, Meta Biomed CO.,LTD, Korea)and 4% taper gutta percha cones together with auxillary cones.

15. The tooth will be sealed by temporary restoration ,Then the details of the endodontic procedure for each patient will be recorded in the patient's procedure chart (Appendix IV) .

16. The patient will be instructed to return to complete the treatment procedures until placing a full-coverage restoration.

17. In case of severe pain or persisting pain patients are instructed to take prescribed analgesic (Ibuprofen 400mg), not to take it less than 6 hours apart ;

Related Conditions & MeSH terms

• Calcium Hydroxide
• Pain, Postoperative

• NCT number: NCT05687760
• Study type: Interventional
• Source: Cairo University
• Status: Not yet recruiting
• Phase: Early Phase 1
• Start date: February 10, 2023
• Completion date: February 10, 2023