Clinical Trial Details
— Status: Recruiting
Administrative data
| NCT number |
NCT05216809 |
| Other study ID # |
ARBT |
| Secondary ID |
|
| Status |
Recruiting |
| Phase |
N/A
|
| First received |
|
| Last updated |
|
| Start date |
February 1, 2022 |
| Est. completion date |
March 2024 |
Study information
| Verified date |
November 2023 |
| Source |
University of Toronto |
| Contact |
Daniel R Moore, PhD |
| Phone |
4169464088 |
| Email |
dr.moore[@]utoronto.ca |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Interventional
|
Clinical Trial Summary
Recent work in the investigators laboratory has examined the ability of a non-invasive 13CO2
breath-test to assess differences in amino acid oxidation rates and net balance in young
healthy males following protein feeding and resistance exercise. The investigators aim to
test the efficacy of this non-invasive 13CO2 breath-test to assess for differences in
anabolic sensitivity between young and older adults following an acute period of habitual and
reduced physical activity.
Description:
Aging is associated with a phenomenon known as anabolic resistance, whereby individuals are
less responsive to anabolic stimuli (i.e., resistance exercise, dietary protein ingestion).
Mild and severe reductions in physical activity are also known to play a key role in the
emergence of anabolic resistance. Therefore, the purpose of this study is to investigate the
ability of a non-invasive 13CO2 breath-test to assess differences in whole-body leucine
oxidation and leucine net balance between younger and older individuals under conditions of
habitual activity and following a period of reduced activity/step-reduction. In this manner,
the ability of the methodology to distinguish anabolic sensitivity between young and older
adult populations will be assessed.
Methods:
Total participant time commitment will span over 4 sessional dates:
Session 1 - Introduction Interested participants will be recruited to engage in a video
conference call to undergo an introductory session. Participants will be provided with a
comprehensive introduction to the study which will explain the research objectives, conduct,
and associated risks. Participants will provide informed consent prior to the collection of
any study information (i.e., anthropometrics and habitual activity levels). Eligibility will
also be screened for at this time.
Session 2 - Body Composition Assessment Participants will consume a bolus of deuterium oxide
(D2O) and provide saliva samples in order to estimate fat-free mass.
The remainder of the study will consist of seven consecutive days, wherein Phase 1 (Habitual
Activity) will be subsequently followed by Phase 2 (Step-Reduction).
Phase 1 - Habitual Activity (Days 1-3) Participants will be asked to engage in their habitual
activity pattern (> 7000 steps/day) while wearing an accelerometer and pedometer.
Participants will be further instructed to maintain their normal dietary patterns and to
track their diets over the course of these three days using diet logs.
Session 3 - Habitual Activity Metabolic Trial (Day 4) On the morning of the fourth day,
following three days of habitual activity, participants will be subjected to the non-invasive
13CO2 breath-test to assess for whole-body leucine oxidation and net balance. This date will
also serve as a lead-in to the subsequent Phase 2.
Phase 2 - Step-Reduction (Days 4-6) Participants will be asked to maintain their normal
dietary patterns and track their dietary intake, but will need to adhere to a reduced daily
step count (<2000 steps/day). Accelerometers and pedometers will be worn again during this
period.
Session 4 - Step-Reduction Metabolic Trial (Day 7) On the morning of the 7th day, following
three days of reduced daily step-count, participants will be subjected again to the
non-invasive 13CO2 breath-test to assess for whole-body leucine oxidation and net balance.
Ultimately, this will allow us to determine whether 3-days of reduced step-count will impart
any changes on anabolic sensitivity.
For each metabolic trial, two urine samples (baseline and pooled enriched) will be collected,
in addition to breath samples collected over a 6-hour post-prandial period at 20-30 minute
intervals. These biological samples will be used to measure tracer excretion and oxidation,
allowing us to determine rates of protein metabolism.
